Increased opioid dependence in a mouse model of panic disorder

[EN] Panic disorder is a highly prevalent neuropsychiatric disorder that shows co-occurrence with substance abuse. Here, we demonstrate that TrkC, the high-affinity receptor for neurotrophin-3, is a key molecule involved in panic disorder and opiate dependence, using a transgenic mouse model (TgNTRK3). Constitutive TrkC overexpression in TgNTRK3 mice dramatically alters spontaneous firing rates of locus coeruleus (LC) neurons and the response of the noradrenergic system to chronic opiate exposure, possibly related to the altered regulation of neurotrophic peptides observed. Notably, TgNTRK3 LC neurons showed an increased firing rate in saline-treated conditions and profound abnormalities in their response to met5-enkephalin. Behaviorally, chronic morphine administration induced a significantly increased withdrawal syndrome in TgNTRK3 mice. In conclusion, we show here that the NT-3/TrkC system is an important regulator of neuronal firing in LC and could contribute to the adaptations of the noradrenergic system in response to chronic opiate exposure. Moreover, our results indicate that TrkC is involved in the molecular and cellular changes in noradrenergic neurons underlying both panic attacks and opiate dependence and support a functional endogenous opioid deficit in panic disorder patients.

Segmentación de mercados y comportamiento del consumidor: el caso de Julen y las zapatillas

Duración (en horas): Más de 50 horas. Destinatario: Estudiante

Nationales Fischereidatenerhebungsprogramm: Aktivitäten und Ausblick

Within the frame of the EU Data Collection Regulation (DCR), Germany is obliged since 2002 to collect basic fisheries data to support the Common Fisheries Policy. Various governmental institutions are involved in the collection of landings and effort data, biological and economic data of the German fisheries. About 200 trips on commercial fishery vessels were sampled from 2002 to 2006. Additional stock data are collected on research surveys. The landings of cod in the recreational fisheries in the North and Baltic Seas were recorded within a pilot study. In order to assess the economic situation of the fishing fleet and processing industry, economic data were collected. The collected data are being stored in a national database and being made available for scientific working groups. At present, the legal regulations within the DCR framework are being reviewed and adapted to the changing requirements of fisheries management.

Multi-drug resistance profiles and the genetic features of Acinetobacter baumannii isolates from Bolivia

Introduction: Acinetobacter baumannii is opportunistic in debilitated hospitalised patients. Because information from some South American countries was previously lacking, this study examined the emergence of multi-resistant A. baumannii in three hospitals in Cochabamba, Bolivia, from 2008 to 2009. Methodology: Multiplex PCR was used to identify the main resistance genes in 15 multi-resistant A. baumannii isolates. RT-PCR was used to measure gene expression. The genetic environment of these genes was also analysed by PCR amplification and sequencing. Minimum inhibitory concentrations were determined for key antibiotics and some were determined in the presence of an efflux pump inhibitor, 1-(1-napthylmethyl) piperazine. Results: Fourteen strains were found to be multi-resistant. Each strain was found to have the bla(OXA-58) gene with the ISAba3-like element upstream, responsible for over-expression of the latter and subsequent carbapenem resistance. Similarly, ISAba1, upstream of the bla(ADC) gene caused over-expression of the latter and cephalosporin resistance; mutations in the gyrA(Ser83 to Leu) and parC (Ser-80 to Phe) genes were commensurate with fluoroquinolone resistance. In addition, the adeA, adeB efflux genes were over-expressed. All 15 isolates were positive for at least two aminoglycoside resistance genes. Conclusion: This is one of the first reports analyzing the multi-drug resistance profile of A. baumannii strains isolated in Bolivia and shows that the over-expression of thebla(OXA-58), bla(ADC) and efflux genes together with aminoglycoside modifying enzymes and mutations in DNA topoisomerases are responsible for the multi-resistance of the bacteria and the subsequent difficulty in treating infections caused by them.

Mechanisms Responsible for the Trophic Effect of Beta-Adrenoceptors on the I-to Current Density in Type 1 Diabetic Rat Cardiomyocytes

Background/Aims: In diabetic ventricular myocytes, transient outward potassium current (I-to) amplitude is severely reduced because of the impaired catecholamine release that characterizes diabetic autonomic neuropathy. Sympathetic nervous system exhibits a trophic effect on I-to since incubation of myocytes with noradrenaline restores current amplitude via beta-adrenoceptor (beta AR) stimulation. Here, we investigate the intracellular signalling pathway though which incubation of diabetic cardiomyocytes with the beta AR agonist isoproterenol recovers I-to amplitude to normal values. Methods: Experiments were performed in ventricular myocytes isolated from streptozotocin-diabetic rats. I-to current was recorded by using the patch-clamp technique. Kv4 channel expression was determined by immunofluorescence. Protein-protein interaction was determined by coimmunoprecipitation. Results: Stimulation of beta AR activates first a G alpha s protein, adenylyl cyclase and Protein Kinase A. PKA-phosphorylated receptor then switches to the G alpha i protein. This leads to the activation of the beta AR-Kinase-1 and further receptor phosphorylation and arrestin dependent internalization. The internalized receptor-arrestin complex recruits and activates cSrc and the MAPK cascade, where Ras, c-Raf1 and finally ERK1/2 mediate the increase in Kv4.2 and Kv4.3 protein abundance in the plasma membrane. Conclusion: beta(2)AR stimulation activates a G alpha s and G alpha i protein dependent pathway where the ERK1/2 modulates the Ito current amplitude and the density of the Kv4.2 and Kv4.2 channels in the plasma membrane upon sympathetic stimulation in diabetic heart.

Cartas enviadas por Francisco R. de Arbulu a Justo Garate en 1970

12 cartas y 2 notas (manuscritas) ; entre 160x215mm y 240x350mm. Ubicación: Caja 1 - Carpeta 41

E-learning y la calibración de ítems de test : teoría de respuesta al ítem versus calibración basada en juicios de expertos. Un estudio jurídico

XVII, 352 p.

Contributions to the analysis and segmentation of remote sensing hyperspectral images

142 p.

Hacia el encuentro de mi anthropos: la muerte, dinamo estructural de la vida

559 p. - Sobresaliente cum laudem por unanimidad. La presidenta del tribunal hace la petición al departamento, en el acto de calificación de la defensa, de publicación de esta tesis por considerarla de interés científico.

Justicia epistémica y epistemología intercultural : una propuesta a partir del SIIDAE, Chiapas

200 p.

Pensamiento romántico naturalista y experiencia procesual en el expresionismo abstracto. Influencia en el informalismo vasco

398 p. : il., col.

Euskal nazionalismoaren diskurtsoa gaur egungo immigrazioaren aurrean

340 p.

Emozioak heziketa fisikoan

Jakin dakigunez, Heziketa Fisikoko irakasle moduan gure helburua ikasleen garapen integrala ematea da. Horretarako, prestatu eta proposatu beharreko edukiak ahalik eta osatuagoak lantzea ezinbestekoa bilakatzen da. Lan horretan, jakina da saio bakoitzean planteatutako ekintza edo jarduera guztiak jokaera motorrak osatutako aspektuak jaso edo landu behar direla. Ekintza hauek, arlo biologikoa, arlo emozionala, harremanen arloa eta arlo kognitiboa barneratzen dituelarik. Lan hau aurrera eramateko orduan, aldiz, arlo emozionalean oinarritu naiz, ikerketaren norabidea alderdi honetara bideratuz . Izan ere, lan honen bitartez alderdi emozionalari garrantzia emateaz gain, Heziketa Fisikoan duen garrantzia azpimarratzen da. Bertan, egoera motor bakoitzean (Psikomotoreak ala soziomotoreak; eta soziomotorretan aurkaritzakoak, lankidetzakoak eta, lankidetza-aurkaritzakoak) ematen diren emozio positiboak ikertzen dira. Ikerketa hau, Bilbon, Irala auzoko Gallego Gorria eskolako bosgarren eta seigarren mailako ikasleei bideratu zaie. Guztira 64 ikaslek parte hartu dute. Ikerketako datuak jasotzeko prozedura ez da metodologia batean oinarritu, baizik eta metodologia kualitatiboaren zein kuantitatiboaren bitartez bideratu da. Horrela, ikerketa ezberdinak burutu eta emaitzak interpretatu ostean datu batzuk jaso ditut: Heziketa Fisikoak emozio positiboak eragiten dizkio ikasleei, jolas soziomotoreen eta psikomotoreen artean, emozio positiboak jolas soziomotorretan ematen direla, eta hauen barnean ematen diren esperientzia ezberdinetatik, ikasleek aurkaritzalankidetza jolasetan biltzen dituztela emozio positiboak.

Lo siniestro, femenino, identidad, imagen, inconsciente, alter ego

873 p.

Aportaciones a la clasificación no supervisada y a su validación. Aplicación a la seguridad informática

220 p.