Características agronômicas, produtividade e qualidade fisiológica da soja safrinha sob semeadura direta, em função da cobertura vegetal e da calagem superficial

A produtividade da soja, no sistema de semeadura direta (SSD), pode ser influenciada pelo tipo de cobertura vegetal que antecede, bem como pela calagem superficial. Todavia, os efeitos dessa técnica sobre os componentes da produção e sobre a qualidade fisiológica das sementes têm sido pouco estudados, principalmente para a soja safrinha sob SPD. Assim, objetivou-se avaliar algumas características agronômicas, os componentes da produção, a produtividade e a qualidade fisiológica da semente da soja cultivada na safrinha, em função de diferentes coberturas vegetais, com e sem calagem superficial, sob SSD. O experimento foi conduzido no ano de 1999/00, na Fazenda Experimental Lageado, FCA/UNESP/Botucatu-SP. O delineamento experimental foi o de blocos casualizados em parcela subdividida, com 4 repetições. As parcelas de 6m x 10m, foram constituídas de sorgo de Guiné vermelho e branco (Sorghum bicolor), milheto (Pennisetum americanum), painço (Panicum dichotomiflorum), vegetação espontânea e sem vegetação. Aos 53 dias após a emergência, as coberturas vegetais foram dessecadas e acamadas, seguindo-se a aplicação superficial de 3,1 t.ha-1 de calcário na metade das parcelas, visando à elevação da V%=70%. Após 2 semanas, realizou-se a semeadura da soja, cv. IAC-19, em safrinha. Determinou-se o estande final, a altura de inserção da primeira vagem, a altura de planta, o número total de vagens e vagens chochas por planta, o número de sementes por vagem total, a massa de 100 sementes e a produtividade. Com relação à qualidade fisiológica das sementes determinou-se a germinação e o vigor, por meio dos testes de envelhecimento artificial, germinação a temperatura subótima, M.S. de plântula e condutividade elétrica. Na implantação do SSD, a cobertura vegetal e a calagem superficial não afetam as características agronômicas, os componentes da produção, a produtividade de sementes e a qualidade fisiológica das sementes de soja cultivadas em safrinha, com exceção da condutividade elétrica.

Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento micelial de Pleurotus ostreatus em resíduo de Simarouba amara

O objetivo deste trabalho foi avaliar o crescimento micelial do cogumelo Pleurotus ostreatus, cultivado na serragem da espécie madeireira Simarouba amara. Avaliaram-se: o efeito das temperaturas de 22, 25, 27, 30 e 35ºC sobre o crescimento micelial de P. ostreatus, nos meios malte-ágar 3% e SDA-MA (infusão da serragem de S. amara, enriquecida com farelo de soja-dextrose-ágar); e o crescimento micelial em substrato de cultivo de serragem de S. amara, com e sem suplementação de farelo de soja, a 25 e 30ºC. O melhor desenvolvimento de P. ostreatus ocorreu em meio malte-ágar 3% a 25ºC. A suplementação de farelo de soja na serragem de S. amara favorece o crescimento micelial.

Modelagem da transferência de massa na remoção do óleo da água produzida por flotação em coluna com uso de tensoativos de origem vegetal

The treatment of oil produced water and its implications are continually under investigation and several questions are related to this subject. In the Northeast Region Brazil, the onshore reservoirs are, in its majority, mature oil fields with high production of water. As this oil produced water has high levels of oil, it cannot be directly discarded into the environment because it represents a risk for contamination of soil, water, and groundwater, or even may cause harm to living bodies. Currently, polyelectrolytes that promote the coalescence of the oil droplets are used to remove the dispersed oil phase, enhancing the effectiveness of the flotation process. The non-biodegradability and high cost of polyelectrolytes are limiting factors for its application. On this context, it is necessary to develop studies for the search of more environmentally friendly products to apply in the flotation process. In this work it is proposed the modeling of the flotation process, in a glass column, using surfactants derived from vegetal oils to replace the polyelectrolytes, as well as to obtain a model that represents the experimental data. In addition, it was made a comparative study between the models described in the literature and the one developed in this research. The obtained results showed that the developed model presented high correlation coefficients when fitting the experimental data (R2 > 0.98), thus proving its efficiency in modeling the experimental data.

Secagem do resíduo de acerola (Malphigia emarginata D.C.): estudo do processo e avaliação do impacto sobre o produto final

Brazil, one of the largest agricultural producers in the world, has managed in recent years to significantly improve its production. However, in response to this advance in the agro-industrial sector, the generation of agro-industrial residues has also increased. New technological alternatives have to be implemented in order to bring economic and rational use of this material and drying is one of the possible choices. Considering the great importance that bioactive compounds present for food science and technology, this research aims to evaluate the air-drying process of acerola residue in a tray convective drier under controlled temperature (60, 70 e 80ºC), air velocity (4.0, 5.0 e 6.0 m/s) and material width (0.5, 0.62 e 0.75 cm) by applying an experimental planning 23 + 3. Based on that, the impact on physical-chemical characteristics, color, bioactive compounds concentration and antioxidant activity of dried acerola waste was evaluated, having the in natura and freeze dried waste as control groups. Dried acerola residue presented natural pigments, mainly carotenoids (143.68 - 68.29 mg/g) and anthocyanins (290.92 - 90.11 mg/100 g), which explain the red and yellow instrumental color parameters observed. The acerola residue powder is also rich in phenolic compounds (3261.11 -2692.60 mgGAEeq/100g), proanthocyanidins (61.33-58.46 eq/100g), ascorbic acid (389.44 739.29 mg/100 g) and DPPH antioxidant activity (20.91 24.72 μg Trolox eq/g). Results show decreased concentration of phenolic compounds, anthocyanins, carotenoids, proanthocyanidins and ascorbic acid caused by the air-drying process. However, even after the observed drying losses, the acerola residue powder can be considered a high value food ingredient, considering the high bioactive compounds concentration found in the final product, as well as the colorimetric characterization and microbiological stability of the dried powder

Secagem do resíduo industrial da acerola em leito de jorro: estudos fluidodinâmicos e análise do desempenho do secador

In this research, the drying process of acerola waste was investigated by using a spouted bed drier. The process was conducted using high density polyethylene inert particles with the objective of producing an ascorbic acid-rich final product. The fruit waste was ground and used to prepare different water-maltodextrin suspensions. Initially, fluidynamical experiments were conducted in order to evaluate the feeding effect on the spouted bed drier fluidynamics behavior. The experimental planning 23 + 3 was used to investigate the effect of the following variables: solids concentration, drying air temperature, intermittence time, production efficiency, solids retention and product losses by elutriation of fine particles on drier walls. The effect of selected independent variables on the drier stability was also evaluated based on a parameter defined as the ratio between the feed suspension volume and the total inert particles volume. Finally, the powder quality was verified in experiments with fixed feed flow and varying air drying temperature, drying air velocity and intermittence time. It was observed that the suspension interferes in the spouted bed drier fluidynamics behavior, and higher air flow is necessary to stabilize the drier. The suspension also promotes the expansion of the spouted bed diameter, decreases the solid circulation and favors the air distribution at the flush area. All variables interfere in the spouted bed performance, and the solids concentration has a major effect on the material retention and losses. The intermittence time also has great effect on the stability and material retention. When it comes to production efficiency, the main effect observed was the drying air temperature. First order models were well adjusted to retention and losses data. The acerola powder presented ascorbic acid levels around 600 to 700 mg/100g. Similar moisture and ascorbic acid levels were obtained for powders obtained by spouted bed and spray drier. However, the powder production efficiency of the spray drier was lower when compared to spouted bed drier. When it comes to energetic analysis, the spray drier process was superior. The results obtained for spouted bed drier are promising and highly dependent on the operational parameters chosen, but in general, it is inferred that this drying process is adequate for paste and suspension drying

Impactos do estresse salino e da cobertura morta nas características químicas do solo e no desenvolvimento do amaranto

Availability of good quality water has been reduced vertiginously, over the last decade, in the world. In some regions, the water resources have high concentration of the dissolved salts, these characteristics of the water make it s use impossible. Water quality can be a limitation for irrigated agriculture, principally in regions of arid or semiarid climate where the water resources are generally saline and are exposed at high evaporation ratio. For that reason, precipitation of the salts occurs near the soil surface and those salts themselves cumulate in the vegetal tissue, reducing the soil fertility and crop production. The adoption of tolerant crop to the water salinity and soil salinity, adaptable to the climatic conditions is other emergent necessity. This work had the goal of studying the effects of four salinity levels of the irrigation water salinity and use of mulch, dried leaves of Forest mangrove (Acacia mangiumWilld), in cultivated soil with amaranth (Amaranthus cruentus, BRS Alegria variety), in greenhouse. It was utilized the transplant of plants to PVC columns, containing 30 kg of silty loam soil, 10 days after emerging, with space of 50 x 50 cm between lines. Treatments were composed by combination of four levels of salinity (0.147; 1.500; 3.000 e 4.500 dS m-1), obtained by addition NaCl (commercial) to irrigation water and soil with and without protection, by mulch. A factorial system 4 x 2 was used with four repetitions, totalizing 32 parcels. The concentrations of nutrients in soil solution have been evaluated, in the dry matter of the vegetal tissue (roots, stem, leaves and raceme residue), at the end of the vegetative cycle. The use of soil protection reduced time for the beginning inflorescence of plants, at the same time, the increase of the salinity delayed this phase of amaranth development. The use of the mulch effectively increased the height, stem diameter, area of the larger leaf, humidity and dry matter content and amaranth grain production. The vegetal species showed salinity tolerance to experimented levels. The adopted treatments did not affect the pH values, exchangeable cation contents, electrical conductivity of soil solution (EC1:5) and saturated extract (ECSE), and Ca+2, Mg+, Fe+2 and Mn+2 contents, in the soil solution. The increase of the salinity concentration in the irrigation water inhibited the mineralization process of the organic matter (OM) and, consequently, the efficiency in the it´s utilization by plants, at the same time, produced increase in the values of the exchangeable sodium percentage (ESP), sodium adsorption ratio (SAR) and potassium adsorption ratio (PAR), in the soil solution. Therefore, the use of the mulch did not affect the first three parameters. The protein and nutrient contents: K+, Ca+2, P, Mg+2 e Cu+2, in amaranth grains, were improved by tillage condition. The raceme residues showed chemical/nutritional composition that makes advantageous its application in animal ration. In this context, it follows that amaranth tolerate the saline stress, of the irrigation water, until 4.500 dS m-1, temperature and relative humidity of the air predominant in the experimental environment

Estudo da produção de pectinase por fermentação em estado sólido utilizando pedúnculo de caju como substrato

Pectinolytic enzymes, or simply pectinases, are complex enzymes that degrade pectic polymers. They have many uses, such as fruit juice extraction and purification, textile fiber treatment and vegetal oil extraction. The aim of this work was to study the kinetics of pectinases production by solid-state fermentation, using dry cashew apple residue as substrate and the microorganism Aspergillus niger CCT 0916. The influence of the initial medium moisture and medium supplementation with a source of nitrogen and phosphorus was evaluated using the factorial experimental planning and response surface methodology. Ammonia sulphate and potassium phosphate were used as nitrogen and phosphorus source, respectively. The variables time of contact (T) and ratio volume solvent/fermented medium (RZ), in systems with and without agitation, were evaluated in order to study the best extraction condition of the produced enzyme. Washed and unwashed cashew apple residues were tested as the growth medium. The unwashed residue was obtained by drying the residue after the extraction of the juice, while the washed residue was obtained by water washing 5 times using the proportion of 1 kg pulp/2 liters of water. Samples were taken every 12 hours for moisture content, pH, protein, reducing sugars, polygalacturonase activity (PG) and viscosity reduction. The physical-chemical composition of the residues had different sugar and pectin levels. For the unwashed residue, the peak activity was reached with 40% of initial moisture content, 1% of nitrogen supplementation without phosphorus addition after 30 hours of process. These conditions led to 16 U/g of PG activity and 82% of viscosity reduction. The calculated models reached similar values to the experimental ones in the same process conditions: 15.55 U/g of PG and 79.57% of viscosity eduction. Similarly, the greatest enzyme production for washed residue was reached with 40% initial moisture content, 1% nitrogen supplementation without phosphorus addition after 22 hours of cultivation. In this condition it was obtained polygalacturonase activity of 9.84 U/g and viscosity reduction of 81.36%. These values are close to experimental values that were of 10.1 U/g and 81%, respectively. The conditions that led to the best PG activity results was the agitated one and the best extraction condition was obtained with 100 minutes of solvent/medium contact and RZ of 5 (mL/g)

Desenvolvimento de sorvete probiótico a base de leite de cabra: estudo da formulação, características físico químicas, sensoriais e viabilidade das bactérias probióticas

This work targetet the caprine ice cream production added with probiotic bacteria Bifidobacterium animalis subsp. lactis. It is divided into two parts. In the first one, four caprine ice cream formulations were evaluated, in which it was used hydrogenated fat (F1 and F3) or fat substitute (F2 and F4) in two different flavors (F1 and F2, passion fruit, F3 and F4, guava). Statistical differences (p<0.05) were detected for their physical-chemical properties, mainly for total solids and fat, but no differences were observed for melting test results. When it went to sensory acceptance, all four ice cream formulations reached high acceptance indexes, mostly formulation F4, which was selected for further studies. In the second part, F4 formulation was prepared with the addition of probiotic bacteria Bifidobacterium animalis subsp. lactis. The growth kinetics was studied and it was observed that the cellular concentration peak was reached after four fermentation hours (10.14 log UFC/g). This time was selected for pre-fermentation procedure and posterior addition at ice cream syrup. In this part of the study, two experimental groups were evaluated: group G1, in which the probiotic addition occurred before the maturation step and group G2, which included a pre-fermentation step and probiotic addition after ice cream maturation. The physical-chemical properties of these two ice cream groups were similar, except for pH, which was higher for group G2 (p<0.05). G1 samples had superior melting rate (3.566 mL/min) and both groups presented microbiological and sanitary results in accordance to current Brazilian legislation. Also, G1 and G2 were considered sensory accepted due to their acceptance indexes higher than 70%. G1 and G2 sensory profiles were similar (p>0.05), and both ice cream samples exhibited high creaminess (6.76 to 6.91) and mouth melting sensation (6.53 to 6.67) scores, while low sandiness scores (0.85 to 0.86) were observed, positive characteristics for this kind of food product. During the first 24 hours after ice cream production, the population of B. animalis subsp. lactis decreased, reaching 7.15 e 6.92 log CFU/g for G1 and G2, respectively. Probiotic bacteria counts fluctuated in ice cream samples during the first 108 days at frozen storage, especially for G2 group. Decreased probiotic viability was observed for G1 samples during the first 35 days of frozen storage, mild variation between 35 and 63 days and stabilized counts were observed after this time. After 21 days at frozen storage, ice cream samples of G1 and G2 groups reached 1.2 x 109 and 1.3 x 109 CFU/portion, respectively. After 108 days under these storage conditions, the survival rate of B. animalis subsp. lactis was 94.26% and 81.10% for G1 and G2 samples, respectively. After simulation of gastroenteric conditions, G2 group reached 9.72 x 105 CFU/portion. Considering the current requirements of Brazilian legislation, which stipulates that functional foods must have minimum probiotic count between 108 and 109 CFU/portion and detectable probiotic bacteria after being submitted to gastroenteric conditions, it is concluded that the ice cream with the addition of Bifidobacterium animalis subsp. lactis made as shown in this work, can be considered as a dairy functional food

Incorporação de resíduo de perfuração em matriz cerâmica: estabilização por solidificação

One of the waste generated during the drilling of oil wells are gravel which are impregnated of drilling fluid. This residue consists of highly toxic chemicals, including toxic metals. This study suggests an alternative process to the treatment of this waste, by incorporating it the form of raw material in the ceramic matrix , and by solidification and stabilize the metals present, Aluminum (Al), Iron (Fe), Manganese (Mn) and Zinc (Zn). The raw materials were characterized by the techniques of X ray fluorescence (FRX), X ray diffraction (DRX), laser granulometry (GL), thermogravimetry (TG) and differential thermal analysis (ADT). To evaluate the percentage of gravel effect the environmental and technological properties were obtained from formulations containing 0, 10 and 20 % by weight of gravel in the ceramic matrix. After sintering at temperatures 1080, 1120 and 1160 °C, the samples were tested for water absorption, the linear shrinkage firing, voltage of rupture and solubility. The results obtained showed that the stabilization by solidification, is a viable alternative to safe disposal of waste drilling. Ceramics products can be used in the manufacture of solid bricks

Incorporação de lodo de esgoto na massa cerâmica para fabricação de tijolos maciços: uma alternativa para a disposição final de resíduo

The disposal of sewage sludge is a growing problem face up to management of sanitary sevices. Otherwise, because its making process characteristic, the Ceramic Industry can tolerate the presence of this wastes as raw material. This study has as object to confirm the use of the sewage sluge in the Ceramic Industry like a sustentable alternative for its disposal. Futhermore, this study quests to evaluate the maximum proportion for incorporation of sludge wich result in technically and enviromentally suitable bricks. For found this proportion, the research consisted of (1) making of bricks in full scale, adde up 0%, 5%,10%, 15%, 20%, 25%,30%, 35% e 40% sludge, with size 220x105x45 mm, hand-molded by rammer and baked by industrial kiln; and (2) tecnical and enviromental evaluation of this bricks, according to Brazilian norms. The raw material uses were two distinct clays come from Goianinha/RN and sewage comes from a septic system tank and pumped into tank vehicle, of Natal/RN. The technical evaluation allowed to conclude the addiotion of the sludge brings about signifcant lost of mass and the water absorption grew up according to increase of sludge: every sludge-amended clay bricks absorved more water than control group. Thus, the compressive strength was signicantly decreased because the increase of sludge: bricks with 5% sludge added lost 45% of strength achieved at control group; the bricks made with 10 and 20% lost almost 70% of bigger strength. With up to 25% sludge added to the bricks, the streght decreased over 90%. Concerning heavy metal leaching, the two maximum proportion wich have tecnical approval, it means bricks sludge added with 15 and 20%, can say there is no risk of enviromental contamination using those bricks. This way, in this work context, it can to conclude the maximum proportion atends the technical and enviromental criterion is 20%

Incorporação do resíduo oriundo do beneficiamento de caulim em concreto asfáltico

The municipal district of Equador-RN is located in an area of great amounts of ores, being your main economical activity the extraction and the kaolin improvement. The main originating from environmental problem that activity is the amount of generated residue, about 70% of the extracted kaolin. The residues are simply piled up in lands of the improvement companies, occupying like this a large area and causing impact in the existent flora. When dry, the residues transform powdered and for the action of the wind, they disperse, polluting the air. Being like this, the present work has as objective evaluates the incorporation of the great residue, originating from of the kaolin improvement, in partial substitution of all the employed aggregates in a conventional mixture of asphalt concrete, which was used in the paving of BR101/RN061 - passage between Ponta Negra and Ares. That evaluation was accomplished in three stages. The first refers to the evaluation of the physical, thermal and mineralogical characteristics of the residue with the intention of to classify it and to define your application as aggregate (small and great). The second refers to the physical characterization of the aggregates and of the asphalt material used in the conventional mixture. And the third, to the evaluation of the mixtures containing residue, which were elaborated starting from the conventional mixture with the gradual incorporation of the residue, from 5 to 40%, in substitution to the part of the conventional aggregates, in way to obtain similar particle size curves the one of the conventional mixture. That evaluation was accomplished through the comparison between the volumetric composition, the mechanical behavior and the susceptibility to the humidity of the mixtures containing residue with the one of the conventional mixture, and with the one of the DNIT specifications. The results show that the great residue originating from of the kaolin improvement has grains of the most varied size, being like this, it can substitute part of all the conventional aggregates and of the filler in an asphalt mixture. Besides, your mineralogical composition presented the same present minerals in the composition of conventional aggregates used in paving. The results evaluation of the volumetric composition of the mixtures containing residue indicates that it can use up to 30% of residue in substitution to the conventional aggregates. The evaluation of the mechanical behavior of those mixtures indicates that the residue increment in the studied mixtures caused an increase of the stability and a reduction of the resistance to the traction. The values obtained in the resistance to the traction meet below the minimum value specified by DNIT, but close to the value obtained in the conventional mixture. When taking in consideration the susceptibility of the same ones to the humidity, the results indicate that she can use up to 25% of residue

Análise da adição de resíduo oriundo do tratamento de esgotos em massa cerâmica utilizada para fabricação de telhas

Sludge of Wastewater Treatment Plants (WTPs) disposal is a problem for any municipality, for this reason the amount of sludge production is now a key issue in selecting treatment methods. It is necessary to investigate new applications for this waste type, due to the restrictions imposed by the environmental organs. The raw materials used in the Red Ceramic, are generally very heterogeneous, for this reason, such materials can tolerate the presence of different types of wastes. In Rio Grande do Norte, the roof tiles production corresponds to 60,61% from the total of ceramic units produced. Due to the importance of the ceramic industry of roof tiles for the state, allied to the environmental problem of the sludge disposal, this work had for objective to verify the possibility of the incorporation of sewage sludge in ceramic body used for production of roof tiles. In the research, sludge originating from drying beds of WTP of the Central Campus from UFRN and clays originating from a ceramic industry from Goianinha/RN were used. The raw materials were characterized by techniques of: analysis of particles distribution by diffraction to laser; real density; consistence limits; chemical analysis by X-ray fluorescence; mineralogical analysis by X-ray diffraction; organic matter; and solids content. Five batches of roof tiles were manufactured in the approximate dosages of 2%, 4%, 6%, 8% and 10%. To evaluate the properties of each final product, tests of water absorption, impermeability, bending strength, leachability and solubility were accomplished. The roof tiles manufactured with sludge presented characteristics similar to the roof tiles without sludge in relation to the environmental risk. The results showed that it is possible to use approximately up to 4% of sludge in ceramic bodies for production of roof tiles. However, it is observed that the high amount of organic matter (71%) present in the sludge is shown as factor that limits the sludge incorporation in ceramic bodies, worsening the quality of the roof tiles. It is necessary the use of mixtures of different raw materials under point of view of the granulometry and of the other chemical and mineralogical properties for the obtaining of a satisfactory mass to the production of ceramic roof tiles

Métodos e estratégias em proteômica e suas aplicações na área vegetal

A implementação da espectrometria de massa (MS) para as análises de peptídeos (MS) e de aminoácidos (MS em tandem ou MS/MS) tornou possível a identificação de centenas de proteínas em experimentos únicos. Uma grande variedade de estratégias está disponível atualmente para o fracionamento e a purificação de amostras, a identificação de proteínas, a quantificação, a análise de modificações pós-traducionais (MPT's) e os estudos de interação. Dessa forma, a proteômica abre novas perspectivas na biologia de plantas com ênfase nos estudos de variabilidade genética, estresses fisiológicos e desenvolvimento de plantas.

Caracterização do comportamento geotécnico de mistura de resíduo de pneus e solo laterítico

A technological alternative for the correct disposal of tires is the use in the construction of embankment with soil and shredded tires. The use of waste tires in tropical soils requires prior knowledge of the properties and limitations of these materials. In this work, the results of an experimental program was devised to characterize the behavior of mixtures of waste tires and a lateritic soil. The residue used in this study is classified as tire buffings with an average size of 1.4 mm. The laboratory program included testing of particle size analysis, Atterberg limits, compaction, direct shear tests, permeability and confined compression tests with pure soil, pure tire and the mixtures. Proportions of 0% (pure soil), 10%, 20%, 40%, 50 % and 100% (pure tire) by weight were used. For the confining stress levels used in the study, the presence of tire residue provided a considerable increase in shear strength of the mixture. The maximum shear strength was obtained for a residue content of 40% by weight. Permeability tests on samples of waste under a confining stress of 100 kPa showed that the permeability increases significantly with increasing residue content until a residue content of 20%. The increase in permeability after that value showed to be negligible. Confined compression tests showed that the soil mixed with tire residue becomes more compressible than the pure soil. The secant constrained modulus (Msec) for the same vertical stress decreases with increasing percentage of residue.