Permissivity of insect cells to Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae.

Recent large scale studies questioning the presence of intracellular bacteria of the Chlamydiales order in ticks and fleas revealed that arthropods, similarly to mammals, reptiles, birds or fishes, can be colonized by Chlamydia-related bacteria with a predominant representation of the Rhabdochlamydiaceae and Parachlamydiaceae families. We thus investigated the permissivity of two insect cell lines towards Waddlia chondrophila, Estrella lausannensis and Parachlamydia acanthamoebae, three bacteria representative of three distinct families within the Chlamydiales order, all documented in ticks and/or in other arthropods. We demonstrated that W. chondrophila and E. lausannensis are able to very efficiently multiply in these insect cell lines. E. lausannensis however induced a rapid cytopathic effect, which somehow restricted its replication. P. acanthamoebae was not able to grow in these cell lines even if inclusions containing a few replicating bacteria could occasionally be observed.

Arrêt du Parlement qui décide que les offices domaniaux ou venaux sont propres de succession et de disposition, lorsqu'ils ont passé du père aux enfants

[Acte. 1709-02-09. Paris]

[Acte de vente des biens, meubles et immeubles provenant de la succession d'Edmée Poussin, mère de Paul Auger, surintendant de la musique de la Chambre du Roi, 9 mai 1638]

Signé : Paul Auger, Henri Auger, Pierre Auger