抗氧化剂对渗透胁迫下沙棘叶片膜脂过氧化的保护作用

以沙棘叶片为材料 ,研究了外源抗氧化物质 Vc、VE、β-胡萝卜素等对 PEG渗透胁迫下细胞膜的保护作用。结果表明 :加入外源 Vc、β-胡萝卜素能有效保护膜系统 ,使膜透性和丙二醛在胁迫条件下增加小于对照 ,尤其在胁迫前期作用明显 ,而对叶绿素的保护作用在后期表现更突出 ,使叶绿素含量维持在较高水平 ;外源 VE 对防止膜透性增加作用不大 ,对减低丙二醛含量与保护叶绿素方面有所贡献。同时也证明沙棘叶片内 Vc在渗透胁迫下含量下降 ,而 Pro含量显著升高。证明这些物质是构成沙棘抗旱性的重要基础。

山杏种皮黑色素的抗氧化活性

对山杏种皮黑色素的抗氧化活性进行评价。结果表明:山杏种皮黑色素可以显著抑制β-胡萝卜素的氧化褪色,其清除自由基活性具有量效性,清除羟自由基能力与2,6-二叔丁基对甲酚(BHT)无显著差异而高于抗坏血酸(VC),清除超氧阴离子能力超过BHT和VC。山杏种皮黑色素既可以作为氢供体,清除DPPH自由基,但清除能力低于BHT和VC,也可作为电子供体,其还原能力高于BHT和VC。

生黑醋酸杆菌山梨醇高浓度发酵的培养特性

调节生黑醋酸杆菌的代谢特性,实现其在高浓度山梨醇下的发酵。改变发酵所用种液的培养特性以及在发酵中期补加山梨醇。在摇瓶培养条件下,通过上述方法可以将Vc一步发酵中底物山梨醇的浓度从23%提高到38%,培养30 h时发酵率达95%,山梨糖产量达360 mg/mL。在优化后的实验条件下山梨糖的发酵产率得到明显提高。

生黑醋酸杆菌在高浓度山梨醇下的半连续发酵特性

目的:调节生黑醋酸杆菌生物和代谢特性,以提高发酵效率。方法:通过改变种液特性,采用半连续培养的方式,对生黑醋酸杆菌在高醇浓度下的生长特性进行了研究。结果:通过优化可以提高VC一步发酵底物山梨醇浓度达38%,32h左右发酵率达95%,山梨糖产量达360mg/ml,半连续培养连续5批之间产糖稳定,没有明显差别。结论:通过优化,有效地提高了山梨糖的产率。

微生物肥料对绿色食品蔬菜品质的影响

对抚顺市顺城区茄子、辣椒、白菜三种蔬菜作物施用微生物肥料进行筛选试验研究,分析比较了三种微生物肥料对蔬菜品质产生的影响。结果表明,肥料C在降低辣椒、白菜和茄子硝酸盐含量,提高辣椒和白菜中Vc含量等方面优于其它两种肥料。

维生素C二步发酵的新组合菌系

以掷孢酵母作为伴生菌与氧化葡萄糖酸杆菌组成新混菌体系 ,对其产酸性能和特点进行了研究。实验室摇瓶结果显示 :新菌系混菌状态不同 ,产酸不同 ,以KGA含量为 4 8,小菌 /掷孢酵母为 31∶1种液接种时 ,最有利于产酸 ,增加接种生物量可提高产酸速度 ,缩短发酵周期 ,但不影响最终产酸量。相同条件下 ,新菌系产酸能力高于现有菌系 ,酸量增加 5mg/ml～ 7mg/ml,发酵周期缩短 6h～ 8h ,酸转化率提高 3%～ 4 % ,最高产酸点pH值下降约 0 5,表现出较大的产酸潜力和可修饰性

维生素C二步发酵中巨大芽孢杆菌对氧化葡萄糖酸杆菌生长和产酸的影响

通过测定氧化葡萄糖酸杆菌转化L－山梨糖中成ZKGA的细胞酶活性、摇瓶发酵及中长变化，研究了Vc:步发酵中巨大茅孢杆菌对氧化葡萄糖酸杆菌生长和产酸作用的影响。结果显示：巨大芽孢杆菌胞外液和胞内液均可促进氧化葡萄糖酸杆菌的增殖，主要表现为缩短其中长周期中的延迟期；巨大芽孢杆菌通过所产生的部分生物活性物质增强氧化葡萄糖酸杆菌产酸的细胞酶活性，促进氧化葡萄糖酸杆菌转化L一山梨糖生成2KGA.

卫星表面光学反射特性建模与仿真研究

在研究空间相机成像效果探测问题时,针对提高探测相机成像真实效果的需要,研究了卫星表面光学反射特性的建模与仿真方法。利用双向反射分布函数(BRDF)仿真卫星表面的光学反射特性,将卫星表面物理特性与其光学反射特性有机地结合起来。在VC.Net环境下开发卫星表面光学反射特性仿真软件,并将软件集成到空间探测相机成像效果仿真平台上。利用平台进行成像仿真实验,生成真实感仿真图片。仿真结果表明,BRDF能够精确仿真卫星表面的光学反射特性,使成像效果仿真更加准确和逼真。

基于HLA的仿真模型管理方法研究

“仿真是一种基于模型的活动”，任何仿真系统都不能离开模型的支持，如果每次开发新的系统都要重新建立模型，费时费力。随着仿真系统的日益复杂，导致仿真模型的结构也日趋复杂，模型管理亦日趋繁琐。因此，研究一种有效的模型管理方法，对于方便模型重用，提高开发效率有着重要的意义。实现对模型的有效管理，首先需要明确管理对象，然后把模型有条理的分类并且规范的描述出来，最后把模型存储在数据库中，供用户重用。论文首先在HLA联邦开发执行过程的基础上，分析和完善了HLA仿真建模体系，明确了HLA仿真中模型的层次；然后总结了现有的模型分类方法，从方便模型统一管理的角度，提出了一种可扩展的模型分类方法；引入了元数据和XML技术，实现了对模型的规范化描述；根据课题研究目的，提出了仿真模型管理系统的设计目标，并设计了系统的体系结构、功能模块和数据结构；最后，综合应用数据库、VC++等技术，实现了模型存储、模型的增、删、改、查以及用户管理等功能，实现了对于模型的统一管理。

Preparation of TiO2 nanocrystallites by hydrolyzing with gaseous water and their photocatalytic activity

TiO2 nanocrystallites were prepared front precursors tetra-n-butyl titanate (Ti(OC4H9)(4)) and titanium tetrachloride (TiCl4). The precursors were hydrolyzed by gaseous water in autoclave, and then calcined at predetermined testing temperatures. The samples were characterized by X-ray diffraction (XRD), thermogravimetry-differential thermal analysis (TG-DTA), field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectra (FT-IR), and UV-vis diffuse reflectance spectra (DRS). The photocatalytic activities of the samples were evaluated by the photobleaching of methylene blue (MB) in aqueous solution and the photocatalytic oxidation of propylene in gas phase at ambient temperature. The results showed that the anatase phase nanocrystalline TiO2 could be obtained at relatively low temperatures (for precursor Ti(OC4H9)4 at I I VC and for TiCl4 at 140 degrees C, respectively), and that the as prepared samples exhibited high photocatalytic activities to photobleach MB in aqueous solution. As the calcination temperatures increasing. the decolor ratio of MB increased and reached the maximum value of nearly 100% at 600 degrees C, and then decreased. The photobleaching of MB by all samples followed the pseudo-first-order kinetics with respect to MB concentration.

Investigation of the self-accelerating process of ascorbic acid by in-situ circular dichroism spectroelectrochemistry

The electrode reaction process of ascorbic (Vc) was studied by in-situ circular dichroic(CD) spectroelectrochemistry with a long optical path thin layer cell on glassy carbon(GC) electrode. The spectroelectrochemical data were analyzed by the double logarithmic method together with nonlinear regression. The results suggested that the mechanism of Ve in pH 7.0 phosphate buffer solution at GC electrode was a two-electron irreversible electrooxidation followed by adsorption of the oxidation product. That is a self-accelerated process. Some kinetic parameters at free and at adsorbed electrode surface, i.e, the formal potentials, E-0' = 0.09 V, E-a(0') = 0.26 +/- 0.02 V; the electron transfer coefficient and number of transfered electron, alpha n = 0.41, alpha(a)n = 0.07;the standard heterogeneous electron transfer rate constant, k(0) = 8.0 x 10(-5) cm.s(-1), k(a)(0) = 1.9 x 10(-4) cm.s(-1) and adsorption constant, beta = 102.6 were also estimated.

Barrier properties of vinylidene chloride copolymers

The permeability coefficients of a series of copolymers of vinylidene chloride (VDC) with methyl acrylate (MA), butyl acrylate (BA) or vinyl chloride (VC) (as comonomer) to oxygen and carbon dioxide have been measured at 1.0 MPa and 30 degrees C, while those to water vapor have been measured at 30 degrees C and 100% relative humidity. All the copolymers are semicrystalline. VDC/MA copolymers have lower melting temperature compared with VDC/BA copolymers, while that melting temperature of VDC/VC copolymer is higher than that of VDC/acrylate copolymers with the same VDC content. The barrier property of the copolymers is predominantly controlled by crystallite, free volume fraction, and cohesive energy. The permeability coefficients of VDC/MA copolymers to oxygen, carbon dioxide, and water vapor were successfully correlated with the ratio of free volume to cohesive energy.

越冬针叶叶绿体超氧阴离子自由基波谱

以Tiron为自旋探针,从越冬针叶叶绿体检出了代表超氧阴离子自由基的TH·ESR信号。此信号可由紫外辐射或光合活性光诱导产生。以单位叶绿素量计算的信号强度比现有文献报导大2—3个数量级。红松信号大于其它针叶树。信号可被外源SOD、VC、乙醇和脱氧所抑制。在一定条件下,随光照增强而增强,12月至5月也表现出逐渐增强的趋势,给自由基诱导红松越冬光氧化伤害理论提供了直接证据。

BIOMIMITICS OF OXIDASE AND OXYGENASE THE ELECTRONIC SPECTRA AND THE OXYGEN UPTAKE KINTICS FOR MPc AND MTPP (M = Fe, Co, Cu, Pc AND TFP - ARE DIANIONS OF PHTHALOCYANIN AND TETRAPHENYLPORPHYRINE, RESPECTIVELY)

The reaction rates of MTPP with oxygen in air are Inas than that with pure oxygen, the ratio is roughly the same as to the partial presence of imygen in air, The influences of S-ligand etbanethiol and O- litand Vc on the above Systems have also been investigated, the former makes the MP hands having more changes and the reaction rate constants becoming greater, the latter has less influence.

L-抗坏血酸与稀土离子作用的NMR研究

利用稀土诱导位移方法研究了水溶液中L-抗坏血酸(Vc)与三价稀土离子的配位作用。在弱酸性条件下二者形成1:1的配合物,稳定常数为8.0M~(-1)。抗坏血酸通过内脂环3位碳上的羟基氧与稀土离子配位,RE~(3+)-O键长为2.0,内脂基和其他碳上的羟基不与稀土离子配位。在配合物中C(3)-C(4)-C(5)-C(6)成反式构象。C(5)-C(6)键与配合物的零偶极位移锥面交叉。