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The unstable combustion that can occur in combustion chambers is a major problem for aeroengines and ground-based industrial gas turbines. Nowadays, CFD provides a flexible, low cost tool to supplement direct measurement. This paper presents simulations of combustion oscillations in a liquid-fuelled experimental rig at the University of Cambridge. Linear acoustic theory was used to describe the acoustic waves propagating upstream and downstream of the combustion zone and to develop inlet and outlet boundary conditions just upstream and downstream of the combustion region enabling the CFD calculation to be efficiently concentrated on the combustion zone. A combustion oscillation was found to occur with its predicted frequency in good agreement with experimental measurements. More details about the unstable combustion can be obtained from the simulation results. The approach developed here is expected to provide a powerful tool for the design and operation of stable combustion systems. Copyright © 2009 by ASME.

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Red snapper (Lutjanus campechanus) in the United States waters of the Gulf of Mexico (GOM) has been considered a single unit stock since management of the species began in 1991. The validity of this assumption is essential to management decisions because measures of growth can differ for nonmixing populations. We examined growth rates, size-at-age, and length and weight information of red snapper collected from the recreational harvests of Alabama (n=2010), Louisiana (n=1905), and Texas (n =1277) from 1999 to 2001. Ages were obtained from 5035 otolith sections and ranged from one to 45 years. Fork length, total weight, and age-frequency distributions differed significantly among all states; Texas, however, had a much higher proportion of smaller, younger fish. All red snapper showed rapid growth until about age 10 years, after which growth slowed considerably. Von Bertalanffy growth models of both mean fork length and mean total weight-at-age predicted significantly smaller fish at age from Texas, whereas no differences were found between Alabama and Louisiana models. Texas red snapper were also shown to differ significantly from both Alabama and Louisiana red snapper in regressions of mean weight at age. Demographic variation in growth rates may indicate the existence of separate management units of red snapper in the GOM. Our data indicate that the red snapper inhabiting the waters off Texas are reaching smaller maximum sizes at a faster rate and have a consistently smaller total weight at age than those collected from Louisiana and Alabama waters. Whether these differences are environmentally induced or are the result of genetic divergence remains to be determined, but they should be considered for future management regulations.

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The U.S. Marine Mammal Protection Act requires that the abundance of marine mammals in U.S. waters be assessed. Because this requirement had not been met for a large portion of the North Atlantic Ocean (U.S. waters south of Maryland), a ship-based, line-transect survey was conducted with a 68 m research ship between Maryland (38.00°N) and central Florida (28.00°N) from the 10-m isobath to the boundary of the U.S. Exclusive Economic Zone. The study area (573,000 km2) was surveyed between 8 July and 17 August 1998. Minimum abundance estimates were based on 4163 km of effort and 217 sightings of at least 13 cetacean species and other taxonomic categories. The most commonly sighted species (number of groups) were bottlenose dolphins, Tursiops truncatus (38); sperm whales, Physeter macrocephalus (29); Atlantic spotted dolphins, Stenella frontalis (28); and Risso’s dolphins, Grampus griseus (22). The most abundant species (abundance; coeffi cient of variation) were Atlantic spotted dolphins (14,438; 0.63); bottlenose dolphins (13,085; 0.40); pantropical spotted dolphins, S. attenuate (12,747; 0.56); striped dolphins, S. coeruleoalba (10,225; 0.91); and Risso’s dolphins (9533; 0.50). The abundance estimate for the Clymene dolphin, S. clymene (6086; 0.93), is the first for the U.S. Atlantic Ocean. Sperm whales were the most abundant large whale (1181; 0.51). Abundances for other species or taxonomic categories ranged from 20 to 5109. There were an estimated 77,139 (0.23) cetaceans in the study area. Bottlenose dolphins and Atlantic spotted dolphins were encountered primarily in continental shelf (<200 m) and continental slope waters (200−2000 m). All other species were generally sighted in oceanic waters (>200 m). The distribution of some species varied north to south. Striped dolphins, Clymene dolphins, and sperm whales were sighted primarily in the northern part of the study area; whereas pantropical spotted dolphins were sighted primarily in the southern portion.

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利用反义技术研究生物代谢途径以及对其生物合成进行调控成为植物次生代谢研究领域内一个重要手段之一,并与新兴的RNAi技术一起成为本领域内重要的研究热点。在植物类异戊二烯代谢途径中存在着羟甲基戊二酰辅酶A还原酶(HMGR)、法呢基焦磷酸合酶(FPS)和鲨烯合酶(SQS)等几种关键的分支酶,他们被认为在异戊二烯类的生物合成中发挥着关键的调节作用。其中,鲨烯合酶处于HMGR和FPS的下游,并与倍半萜合酶等利用共同的前体-法呢基二磷酸(FPP),以FPP起始合成一系列的下游产物。因此,FPP成为类异戊二烯途径中的关键调节点之一。本论文基于此目的,利用反义技术研究了FPP合成鲨烯这一途径受到抑制对其他以FPP为生物合成前体的代谢支路的影响。 利用植物双元转化载体pBI121,将青蒿中鲨烯合酶基因的cDNA(约1.5kb)序列插入到pBI121中,取代原有的GUS序列,构建成植物转化载体pBIASS。以根癌农杆菌为介导,将青蒿鲨烯合酶反义基因序列导入到烟草,整合到其基因组中 ,成功获得转基因植株。对转基因烟草进行分子检测表明,外源鲨烯合酶基因的序列已经稳定整合到烟草基因组中,并对内源的烟草鲨烯合酶基因表达产生影响。转基因烟草中检测到内源鲨烯合酶基因的mRNA的水平降低。对鲨烯合酶下游产物之一的胆固醇的含量分析显示,活性减低的鲨烯合酶使胆固醇的生物合成下降约40%左右。同时,另一条以FPP为共同前体的二萜代谢途径产物之一GA3的含量得到了提高,比对照提高约30%。

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东灵山地区年均降水量659.7mm,单次降水以雨量小、雨强低的降水为主。水汽压(年均17.7mb)、相对湿度(年均66%)的季节变化呈现生长季高、冬季低的趋势。年均蒸发量1019.5mm;气温、风速、日照时间和水压与月蒸发量和日蒸量相关显著;气温、日照时间和水压分别在11-6月、7-8月和9-10月为决定蒸发量的首要因子。枯枝落叶层、土壤层湿度主要受前十日降水量和坡向影响。 植物体含水量生长季节较高,冬季较低;含水量随径级的增大而降低。六个灌木树种的平均含水量大小顺序为:毛榛(48.62%)最高荆条(36.32%)最低;七个乔木树种水分含量为油松,56.14%;蒙椴,54.19%;华北落叶松,52.91%;五角枫,43.64%;辽东栎,41.87%;棘皮桦,41.13%;大叶白腊,37.79%。几种植被类型的储水量为:辽东栎林,82.08mm;华北落叶松林,47.35mm;混交林,34.60mm;油松林,31.33mm;灌丛,12.40mm。各树种叶片日最低水势的季节均值为:辽东栎,-16.1bar;五角枫,-15.8bar;大叶白腊,-15.1bar;糠椴,-13.4bar;棘皮桦,-12.3bar;蒙椴,-12.2bar。叶片水势的日间变化均呈一“V”形曲线;光照在叶片水势的日间变化中起着决定性作用。 96年各树种平均单株树干茎流量为辽东栎,30.3mm(4.19%);华北落叶松,16.1mm(2.22%);油松,8.9mm(1.23%);棘皮桦,2.9mm(0.40%)。两个生长季各林分冠层的水量平衡为:辽东栎林,树干流茎量101.87mm(9.18%),穿透降水量823.08mm(74.15%),截留量185.05mm(16.67%);华北落叶松林,树干径流量66.88mm(6.03%),穿透降水量836.92mm(75.40%),截留量206.20mm(18.58);混交林,树干径流量50.13(4.52%),穿透降水量846.78mm(76.29%),截留量212.20mm(19.12%);油松林,树干径流量33.90mm(3.05%),穿透降水量934.88mm(84.22%),截留量141.22mm(12.72%)。多元回归分析表明,树干流茎量S与降水量P和前24小时降水量P_1呈显著正相关关系;穿透降水量T与降水量P和最大雨强M正相关显著。附加截留量与降水时间成正比。 枯枝落叶层的生物量为:油松林,25.56t/hm~2;华北落叶松林20.01t/hm~2;辽东栎林,8.31t/hm~2;混交林,7.98t/hm~2。枯枝落叶层的平均实际持水量和有效持水量均以油松林最大,其次是华北落叶松林,而混交林和辽东栎林较低;枯枝落叶层的实际持水量和有效持水量的季节变化分别与前十日降水量P10成正相关和负相关关系。枯枝落叶层的截留量为油松林>华北落叶松林>辽东栎林>混交林;油松林(145.632mm和90.800mm)混交林(61.816mm和54.504mm)。油松林、辽东栎林、混交林和华北落叶松林去除枯枝落叶层后,土壤入渗量比对照平均降低100mm以上;表层土壤含水量分别比对照土壤下降了6.26、18.26、15.06和15.07个百分点。地表径流量分别增加了,辽东栎林34.299mm(603%)和15.816mm(525%);油松林14.593mm(732%)和10.584mm(1321%);混交林12.004mm(181%)和7.275mm(364%);华北落叶松林3.555mm(118%),3.275mm(229%)。96年生长季,各土壤流失量分别增加了:油松林172.751t/hm~2(124倍);辽东栎林836.500t/hm~2(119倍);混交林172.499t/hm~2(47倍);华北落叶松林11.557t/hm~2(11倍)。表层土壤容重分别增加了:油松林15.0%和20.6%,辽东栎林18.4%和28.2%,混交林11.5%和38.5%,华北落叶松林4.3%和17.1%。 0-60cm深度土壤容重平均值的大小顺序为:草地>灌丛>辽东栎林>油松林>混交林>华北落叶松林;而土壤孔隙度的大小顺序为华北落叶松林>混交林>油松林>辽东栎林>灌丛>草地。两个生长季为土壤实际储水量的均值:油松林,124.45mm,78.62mm;辽东栎林,131.23mm,87.72mm;混交林,180.41mm,113.90mm;华北落叶松林,165.53mm,127.95mm;灌丛,172.50mm,89.81mm;草地,152.92mm,89.59 mm分别比干旱年份97年高出45.83mm、43.51mm、51.63mm、37.58mm、82.69mm和63.33mm。两个生长季的地表径流量为草地,30.930mm(2.79%);灌丛,16.321mm(147%);油松林,2.911mm(0.26%);辽东栎林,8.703mm(0.78%);混交林,8.625mm(0.78%);华北落叶松林,4.447mm(0.40%)。油松林、混交林和华北落叶松林地表径流量与降水量P(mm)和最大雨强(mm/h)正相关显著;而辽东栎林、灌丛和草地的地表径流量则与降水量P(mm)、平均雨强Q(mm/hr)和最大雨强M(mm/hr)三者之间呈显著正相关关系。与草地相比(1220.093kg/hm~2,100%),灌丛、辽东栎林、混交林、油松林和华北落叶松林96年生长季的土壤流失量分别降低了85.05%、94.26%、96.99%、98.86和99.14%。 降水量是影响小流域径流量时间变化的主要因素;南沟和马牙石沟96年的径流量分别是97年的8.19倍和7.87倍,而径流深(46.25mm,52.75mm)分别比97年(5.65mm,6.70mm)高出40.60mm和46.05mm。两个小流域由于面积的差异而使南沟两年的径流量分别比马牙石沟高出2773.136m~3(13.15%)和235.434m~3(8.79%)。96年和97年马牙石沟径流深比南沟高出6.5mm(14.05%)和1.05mm(18.58%)。在地处大陆性季风气候区的东灵山地区,用0.010m~3/min/km~2/hr能较好地分割小流域的洪峰和基流。在五次暴雨水文曲线中,马牙石沟的快速径流量分别比南沟高出25.00%到143.33%。五次洪水水文响应R的平均值南沟为0.218%,马牙石沟为0.404%;与海洋性气候地区相比,东灵山地区小流域的R值要低一到两个数量级。马牙石沟洪峰流量Qp的平均值为418.772L/min要比南沟(281.191L/min)大48.9%。东灵山地区小流域的洪水径流过程可分为三种类型。