Abundance and biomass of phytoplankton in the Black Sea near Gelendzhik in 2002-2005

It is shown that in 2002-2005 mass development of coccolithofore Emiliania huxleyi on the Gelendzhik shelf (northeast Black Sea) occurred annually and in May-June its abundance reached 1500000 cells/l. In 2004-2005 bloom of E. huxleyi was accompanied by mass development of diatom alga Chaetoceros subtilis var. abnormis f. simplex (600000-900000 cells/l). For the first time it was registered as a dominating form of Black Sea phytoplankton. Small flagellates and picoplankton algae played a noticeable role in phytoplankton throughout the entire period of the studies. Meanwhile in the early summer period the bulk of biomass consisted of coccolithophores (50-60%), while in the late summer period diatomaceous algae dominated (50-70%). Among ecological factors that favor coccolithophore development one may note microstratification of the upper mixed layer at a high illumination level and high temperature in surface waters (18-21°C). Terrigenous runoff during the rainy period had a negative effect on E. huxleyi development, while storms dispersed the population over the upper mixed layer. A wind-induced near-shore upwelling stimulated development of diatoms.

Phyto- and zooplankton of the southeastern Barents Sea in April 2000

Spring bloom of cold-water centric and pennate diatoms was observed in two different areas of the southeastern Barents Sea in April 2000: ice-free waters off the Kolguev Island northern shelf and the eastern Pechora Sea near the Karskie Vorota (Kara Gate) Straight in polynyas and ice-free patches in one-year-old ice. Maximal values of phytoplankton abundance and biomass were found at the ice edge. The bloom was localized in shallow water areas with depths less than 50 m in mixing zones of waters of different origin: warm Atlantic, cold coastal, and Arctic (Litke current) waters. Ice melting was among factors inducing the phytoplankton bloom. Each area had a specific phytocoenosis, whose structure was determined by water origin and ice conditions. In the western Kara Sea, under a solid (up to 30 cm thick) ice cover (i.e., under conditions of a hydrological winter), a spring phytoplankton succession was observed from its initial stage. In areas located close to the ice-cover edge, simultaneously with the mass phytoplankton bloom, the early spring zoocoenosis development manifested itself in mass spawning of euphausiids and mass appearance of Cirripedia nauplii and bottom polychaete larvae.

Carbon cycling in the northern Humboldt Current off Chile

The structure of the zooplankton foodweb and their dominant carbon fluxes were studied in the upwelling system off northern Chile (Mejillones Bay; 23°S) between October 2000 and December 2002. High primary production (PP) rates (18 gC/m**2 d) were mostly due to the net-phytoplankton size fraction (>23 µm). High PP has been traditionally associated with the wind-driven upwelling fertilizing effect of equatorial subsurface waters, which favour development of a short food chain dominated by a few small clupeiform fish species. The objective of the present work was to study the trophic carbon flow through the first step of this 'classical chain' (from phytoplankton to primary consumers such as copepods and euphausiids) and the carbon flow towards the gelatinous web composed of both filter-feeding and carnivorous zooplankton. To accomplish this objective, feeding experiments with copepods, appendicularians, ctenophores, and chaetognaths were conducted using naturally occurring plankton prey assemblages. Throughout the study, the total carbon ingestion rates showed that the dominant appendicularian species and small copepods consumed an average of 7 and 5 µgC/ind d, respectively. In addition, copepods ingested particles mainly in the size range of nano- and microplankton, whereas appendicularians ingested in the range of pico- and nanoplankton. Small copepods and appendicularians removed a small fraction of total daily PP (range 6-11%). However, when the pico- + nanoplankton fractions were the major contributors to total PP (oligotrophic conditions), grazing by small copepods increased markedly to 86% of total PP. Under these more oligotrophic conditions, the euphausiids grazing increased as well, but only reached values lower than 5% of total PP. During this study, chaetognaths and ctenophores ingested an average of 1 and 14 copepods/ind d, respectively. In terms of biomass consumed, the potential impact of carnivorous gelatinous zooplankton on the small-size copepod community (preferred prey) was important (2-12% of biomass removed daily). However, their impact produced more significant results on copepod abundance (up to 33%), which suggests that carnivorous gelatinous zooplankton may even modulate (control) the abundance of some species as well as the size structure of the copepod community.

Nanoflagellates (mixotrophs, heterotrophs and autotrophs) in the oligotrophic eastern Mediterranean

The vertical distribution (0 to 100 m) and abundance of nanoflagellates were examined in the oligotrophic Aegean Sea (east Mediterranean) in early spring (south basin) and late summer (north and south basins) of 1997 in the framework of the MATER project (Mass Transfer and Ecosystem Response). Different trophic types of nanoflagellates (mixotrophic, heterotrophic, and phototrophic) were identified based on the possession of chloroplasts and the consumption of Fluorescently Labelled Minicells (FLM). Bacterial production (leucine method) was compared with bacterivory estimated from FLM consumption. We found that mixotrophic nanoflagellates played a small role as bacterivores relative to heterotrophic nanoflagellates and total bacterivory roughly balanced bacterial production. In early spring with cool (14.2°C) well-mixed water columns, flagellate concentrations were lowest, phototrophic flagellates were the dominant group and concentrations varied little with depth. Average concentrations of mixotrophs, heterotrophs and autotrophs were 0.07, 0.34, and 0.64 x 103 cells/ml, respectively. Bacterial production in the 0 to 100 m layer averaged about 0.74 µg C/l/d. Estimated nanoflagellate bacterivory from FLM ingestion accounted for 40% of bacterial production with mixotrophic nanoflagellates consuming 5% of bacterial production. In late summer, total nanoflagellate concentrations were higher. Average concentrations of mixotrophs, heterotrophs and autotrophs were 0.09, 1.14, and 0.66 x 103 cells/ml, respectively, in the southern basin and 0.09, 1.1, and 0.98 x 103 cells/ml, respectively, in the northern basin. In September, bacterial production for both basins roughly balanced estimated nanoflagellate consumption. Similar to the March estimates, mixotrophic nanoflagellates accounted for about 5% of nanoflagellate bacterivory. In a nutrient enrichment experiment in March, treatments including phosphorus resulted in increased bacterial production and reductions in identifiable mixotrophs.

Phytoplankton pigments and biomass in the northeastern Sea of Okhotsk from March 23 to April 14, 1998

Studies were carried out in the northeastern Sea of Okhotsk, in the zone of interaction of the West Kamchatka and Compensating Currents at the beginning of spring seasonal succession from March 23 to April 14,1998. Samples for analysis of pigmentary and species compositions of phytoplankton were taken from the sea surface layer, depth 0.5 m. To reduce influence of micropatchiness on phytoplankon distribution at each station subsamples 0.7-1 l were collected every 50-100 m. These subsamples were used to make integral samples 4.5-8.0 l. Phytoplankton biomass and concentration of chlorophyll a varied from 18.7 to 490.9 mg/m**3 and from 0.129 to 2.422 mg/m**3, respectively. Total concentration of phytoplankton pigments varied from 0.622 to 6.679 mg/m**3. In samples studied 51 species of microalgae from 5 orders were found. In terms of the number of species, Bacillariophyta (31 species) and Dinophyta (15 species) prevailed. Diatomaceous algae make up more than 80% of the total phytoplankton biomass in waters of the Compensating Current, from 50 to 80% in intermediate waters, and less than 50% in waters of the West Kamchatka Current. Phytoplankton populations consisting primarily of diatoms were characterized by very low chlorophyll a to biomass ratio (0.1 %). It is three times lower than the ratio observed in phytoplankton populations that were close by species composition and size composition in this area in the late April-early May 1996.