Análise estereológica e bioquímica do tecido conjuntivo e muscular no corpo cavernoso adjacente à placa fibrosa da doença de Peyronie

Os estudos sobre a doença de Peyronie geralmente estão centrados em analises da placa fibrosa que se forma na túnica albugínea. Devido a esta reação fibrótica mediada por vários fatores solúveis inflamatórios o tecido conjuntivo adjacente também pode ser afetado. Este efeito secundário pode, por exemplo, explicar a disfunção erétil que ocorre na doença. Foram obtidas biopsias do corpo cavernoso adjacente a placa fibrótica e parte da própria placa de 7 pacientes com a doença de Peyronie (média de idade 48.3 anos). As amostras do grupo controle foram obtidas de forma semelhante durante a autopsia de 5 indivíduos (média de idade 52,3 anos). O material foi submetido a técnicas histoquímicas: H&E, Van Gieson, técnicas imunohistoquímicas: Anti-alfa actina, Anti-elastina e métodos bioquímicos para a dosagem do colágeno total. A quantificação foi feita através de métodos estereológicos. No corpo cavernoso foi observada uma redução estatisticamente significativa de fibras do sistema elástico de pacientes com a doença de Peyronie (19,49% 3,27% vs 23,56% 1,87%; p < 0,05), O colágeno e o músculo liso não apresentaram variação quantitativa quando comparado ao grupo controle. No músculo liso (34,46% 2,06% vs 38,38% 3,17%) e tecido conjuntivo (35,39% 6,15% vs 38,02% 5,03%). A densidade volumétrica das fibras do sistema elástico na placa fibrótica foi diminuída em 38,3% comparada a túnica albugínea normal (20,25% 5,49% vs 32,81% 4,75%; p<0,02) e a concentração de colágeno no corpo cavernoso do grupo controle (77,94 24,26 μg/mg) e doença de Peyronie (66,57 19,39 μg/mg) não diferem significativamente. Os cortes corados com Vermelho de Picrosirius revelaram que no corpo cavernoso normal cores associadas ao colágeno encontravam-se homogeneamente distribuídas. Na doença de Peyronie o colágeno apresentava uma desorientação. A análise quantitativa indicou que o colágeno do corpo cavernoso adjacente à placa fibrótica não estava afetado, embora sua organização estivesse notoriamente alterada. As fibras do sistema elástico do corpo cavernoso estavam reduzidas e uma modificação similar foi encontrada na placa fibrótica da túnica albugínea. Estes resultados sugerem que, embora ocorram primariamente na túnica albugínea, a placa fibrótica da doença de Peyronie pode induzir mudanças no corpo cavernoso adjacente.

圆口铜鱼活鱼运输方法比较

采用尼龙袋充氧密封运输、橡皮袋充氧密封运输、塑料桶充气运输和鱼箱充氧运输4种方法,进行了圆口铜鱼的活鱼运输试验,运输成活率在75.0%~100%。在运输过程中对麻醉剂的使用进行了初步尝试,并对运输后鱼体进行消毒处理,其5 d内的成活率在78.1%~100%。为制定合适的圆口铜鱼活鱼运输方式和开展下一步的实验奠定了基础。

Monitoring organochlorine pesticides and polychlorinated biphenyls in Baiyangdian Lake using microbial communities

We used polyurethane foam units (PFUs) to collect persistent organic pollutants (POPs) from four sites in Baiyangdian Lake in July 2003. Following extraction from the PFUs, relative concentrations of seven organochlorine pesticides (OCPs) and ten polychlorinated biphenyls (PCBs) were determined by gas chromatography. OCPs and PCBs were detected in the microbial communities from all the four sampling stations. In terms of the total concentration of POPs (OCPs+ PCBs), two river estuary stations had more POP (18.45 mu g/L and 9.77 mu g/L) than the two mid-lake stations (4.75 mu g/L and 5.21 mu g/L), indicating that Baiyangdian Lake was significantly impacted by inflow from the Fu River and Baigou River.

Effects of polyaluminum chloride and copper sulfate on phosphorus and UV254 under different anoxic levels

Experimental sediments and water from shallow, eutrophic Dianchi Lakes were treated in a controlled laboratory microcosm using different chemicals under different anoxic levels. This study revealed that the polyaluminum chloride (PAC) was able to inhibit the phosphorus release and decrease the UV254 value at any anoxic level. When the DO concentrations were between 0.76-0.95 mg(.) L-1, the UV(254)value, total phosphorus (TP), and total dissolved phosphorus (TDP) in the water column were decreased by 71.93%, 87.12% and 64.24% respectively. The UV254, TP, and TDP were also decreased by 72.94%, 70.87% and 50.76% respectively at the levels of 4.56-5.32mg(.)L(-1) of DO concentrations. The treatment effects of TP and TDP in the water column using copper sulfate however were not as efficient as the PAC treatment. The UV254 value was increased with the addition of copper sulfate at every anoxic level tested but the chlorophyll-a (Chl-a) content was decreased rapidly and efficiently by copper sulfate more than the treatment by PAC. When the DO concentrations were 0.76-0.86mg(.)L(-1) and 4.75-5.14mg(.)L(-1), the Chl-a concentrations were decreased by 84.87% and 75.07% respectively through copper sulfate treatment. With additions of PAC and copper sulfate, the phosphorus fractions in sediments were shifted forward to the favorable shapes that have little ability of release. The TP concentrations in sediments were increased after treatment via PAC and copper sulfate. Under anoxic conditions, most of the BD-P (Fe-P) to NaOH-P (Al-P) was converted using the recommended PAC dose in BD-P rich sediment. Similar to the PAC, the copper sulfate also could flocculate the exchange phosphorus from sediment to overlying water. Overall though, the effects of copper sulfate treatment were not better than that of the PAC.

Analysis of semiconductor microlasers with an equilateral triangle resonator by rate equations

Semiconductor microlasers with an equilateral triangle resonator (ETR) are analyzed by rate equations with the mode lifetimes calculated by the finite-difference time-domain technique and the Pade approximation. A gain spectrum based on the relation of the gain spectrum and the spontaneous emission spectrum is proposed for considering the mode selection in a wide wavelength span. For an ETR microlaser with the side length of about 5 mum, we find that single fundamental mode operation at about 1.55 mum can be obtained as the side length increases from 4.75 to 5.05 mum. The corresponding wavelength tuning range is 93 nm, and the threshold current is about 0.1 to 0.4 mA.

Terahertz Quantum Cascade Laser Operating at 2.94 THz

The development of quantum cascade laser at 2.94 THz is reported. The laser structure is based on a bound-to-continuum active region and a semi-insulating surface-plasmon waveguide. Lasing is observed up to a heat-sink temperature of 70 K in pulsed mode with light power of 4.75 mW at 10 K and 1 mW at 70 K. A threshold current density of 296.5 A/cm(2) and an internal quantum efficiency of 1.57 x 10(-2) per cascade period are also observed at 10 K. The characteristic temperature of this laser is extracted to be T-0 = 57.5 K.

维生素C二步混菌发酵中伴生菌作用机制的研究

该文对维生素C“二步发酵法”第二步混合发酵中伴生菌对产酸菌——氧化葡萄糖酸杆菌作用机制进行了研究.除巨大芽孢杆菌外，还选用另外几种芽孢杆菌及酵母菌作为产酸菌的伴生菌.各伴生菌及其胞外液对产酸菌生长和2-酮基-L-古龙酸合成能力均有刺激作用，表明产酸菌的伴生菌具有广谱性.利用膜超滤浓缩、柱层析及电泳等技术，从伴生菌B2980胞外液中分离纯化出达电泳均一纯的高纯度活性蛋白质样品，并对其部分基本特性进行分析.结果表明：该活性物质是分子量为36300道尔顿，等电点为4.75的酸性蛋白质，并且是由一个亚基构成的单体蛋白.活性蛋白质的结晶呈规则的菱形形状，其水溶液显著提高产酸菌中SDH酶活性.试验中依伴生菌胞外液对产酸菌中SDH酶活性的作用，建立了该研究中目标活性物质的快速检测方法.