El arte de la adaptación cinematográfica: Dead Man Walking

Eterio Pajares, Raquel Merino y José Miguel Santamaría (eds.)

Manifestação solicitada pelo TCU à CONOF/CD acerca de relatório preliminar, relativo à consulta sobre a aplicação da Lei Complementar nº 141, de 2012, formulada pela Comissão de Seguridade Social e Família/CD : TC 046.061/2012‐6

Avalia o relatório elaborado pelo TCU acerca da consulta formulada pela CSSF/CD(TC 046.061/2012‐6). Procura a pacificação de entendimento no âmbito do Congresso Nacional.

The central nervous control of walking in the locust, Schistocerca Americana

Rhythmic motor behaviors in all animals appear to be under the control of "central pattern generator" circuits, neural circuits which can produce output patterns appropriate for behavior even when isolated from their normal peripheral inputs. Insects have been a useful model system in which to study the control of legged terrestrial locomotion. Much is known about walking in insects at the behavioral level, but to date there has been no clear demonstration that a central pattern generator for walking exists. The focus of this thesis is to explore the central neural basis for locomotion in the locust, Schistocerca americana.

Rhythmic motor patterns could be evoked in leg motor neurons of isolated thoracic ganglia of locusts by the muscarinic agonist pilocarpine. These motor patterns would be appropriate for the movement of single legs during walking. Rhythmic patterns could be evoked in all three thoracic ganglia, but the segmental rhythms differed in their sensitivities to pilocarpine, their frequencies, and the phase relationships of motor neuron antagonists. These different patterns could be generated by a simple adaptable model circuit, which was both simulated and implemented in VLSI hardware. The intersegmental coordination of leg motor rhythms was then examined in preparations of isolated chains of thoracic ganglia. Correlations between motor patterns in different thoracic ganglia indicated that central coupling between segmental pattern generators is likely to contribute to the coordination of the legs during walking.

The work described here clearly demonstrates that segmental pattern generators for walking exist in insects. The pattern generators produce motor outputs which are likely to contribute to the coordination of the joints of a limb, as well as the coordination of different limbs. These studies lay the groundwork for further studies to determine the relative contributions of central and sensory neural mechanisms to terrestrial walking.

In vitro phagocytic study of blood leucocytes and peritoneal macrophages of walking catfish Clarias batrachus ( Ham.) against Aeromonas hydrophila and Escherichia coli.

In observation of in vitro phagocytic activity against Aeromonas hydrophila isolate 34k (a virulent form) and Escherichia coli (an avirulent bacteria) of neutrophil- and monocyte-like cells of walking catfish Clarias batrachus showed phagocytosis. N eutrophils and monocytes phagocytized the avirulent form of bacterial isolate more than the virulent one. Other blood leucocytes did not show phagocytosis. Peritoneal macrophage of the fish were separated by glycogen elicitation and the macrophages were being adhered on plastic cover slips for studying their in vitro phagocytic activity. Most of the cells were alive after adherence and showed phagocytosis against the virulent and avirulent bacteria. The percent phagocytosis and phagocytic index were higher against the avirulent E. coli than the virulent A. hydrophila.

Effect of low cost feed on the production of walking catfish, Clarias batrachus in farmer's ponds

A feeding trial was conducted for six months in farmer's ponds to assess the performance of BFRI formulated catfish feed on the growth and survival of Clarias batrachus (L.). Nine interested farmers and their ponds (size range: 10-15 dec) in the Barera union of Mymensingh Sadar were selected. The ponds were divided into 3 treatments each with 3 replications. Among the three treatment diets, two diets - traditional (F1) and BFRI formulated (F3) were prepared by using low cost agro-based locally available ingredients and the commercial diets was Saudi-Bangla Grower-1 (F2). The diets were designed as F1, F2 and F3 for traditional (20.40% protein), Commercial (31% protein) and BFRI formulated (30.44% protein) diets respectively. The fingerlings of catfish (7.3 g) were collected from local fish vendors and stocked at the rate of 100/dec. Feeding rates were adjusted by weight after fortnightly sampling of fish. Feeding rate were 10 and 8% of the total body weight respectively for 1st, 2nd month and 5% for the rest of the experimental period. The range of some selected water quality parameters were as follows: dissolved oxygen 4.0 - 7.4 mg/l, temperature 24.0°- 33.9°C, pH 6.8 - 8.00, and transparency 17.0 - 32.00 cm. Which showed suitability of the ponds for rearing fish. At the end of the experiment, significantly highest gain (p<0.05) in weight (1210.96% ±87) and lowest gain in weight (865.25% ±90) were observed in the group of fish fed on diets F3 and F1 respectively. However, no significant differences in growth (p>0.05) was observed in fish fed on commercial diet (F2) and BFRI formulated diet (F3). The FCR value ranged between 2.00 and 2.80 with the traditional diet (F1) showing significantly lower FCR. The total production of fish ranged between 1398.08 and 2145.34 kg/ha with F3 diet resulting in the highest production and net profit. A simple economic analysis showed that fish fed with BFRI formulated (F3) diet resulted in the highest net profit in farmer's pond.

Nursery management system of stinging catfish, Heteropneustes fossilis and walking catfish, Clarias batrachus in Bangladesh

A total of four experiments were conducted to develop nursery management system for the two important native catfishes viz. stinging catfish, Heteropneustes fossilis and walking catfish, Clarias batrachus during 2003 and 2004. Two experiments were conducted in on-station condition to determine stocking density efficacy in hapa and in earthen mini ponds for H. fossilis. This was followed by on-farm trial on stocking density in earthen mini ponds. In hapa, the highest survival rate was 60% for H. fosssilis at stocking density of 100/square meter; followed by 54.5 and 50% at stocking densities of 150 and 200/square meter, respectively. Similarly, gain in weight and length was also better in lower stocking densities. Similar survival rate (45 to 55%) was observed in three densities in earthen mini ponds. However, gain in weight and length showed no significant (p<0.05) differences. H. fossilis, C. batrachus showed almost similar survival rates (57.5-59.0%) in all the three stocking densities (100, 150 and 200/square meter) in earthen mini ponds in on-station condition. In terms of gain in weight and length showed no significant (p<0.05) differences in pond conditions. Almost similar result was obtained in terms of growth and survival in on-farm condition.

Dietary protein and energy interations: an approach to optimizing dietary protein to energy ratio in walking catfish, Clarias batrachus

An 8 weeks feeding trial was conducted in a static indoor rearing system to investigate protein to energy ratio (PIE ratio) in walking catfish Clarias batrachus. Six fishmeal based diets of two protein levels (25 and 35%), each with three lipid levels (5, 10 and 15%) resulted in P/E ratios ranging from 13.57 to 21.97 mg protein kJˉ¹ gross energy (GE) were fed to 50 fish in triplicate. Fish were fed 6% of their body weight three times per day adjusted fortnightly. Significantly higher (p<0.05) growth rates in terms of weight gain, % weight gain and specific growth rate (SGR) were evident in fish fed with higher protein diet. The highest growth rate was found by fish fed 35% protein, 17.06 kJˉ¹GE with a P/E ratio of 20.55 mg protein kJˉ¹GE. Significantly better (p<0.05) feed conversion ratio (FCR) was also evident in fish fed with higher protein diet and best FCR was found by fish fed 35% protein, 10% lipid, 17.06 kJˉ¹GE with a P/E ratio of 20.55 mg protein kJˉ¹GE. Significantly indifferent (p>0.05) values of protein utilisation were found in between the both (higher and lower) protein diets. Higher lipid deposition (p<0.05) in whole body was observed with increasing dietary lipid level at each protein diet and as higher (p<0.05) for the lower protein diets. The study reveals that C. batrachus performed best the diet containing 35%, 17.06 kJ gˉ¹ and 20.55 mg protein kJ gˉ¹ GE protein, gross energy and P/E ratio respectively.

Telemetric recordings of single neuron activity and visual scenes in monkeys walking in an open field

This paper describes a portable recording system and methods for obtaining chronic recordings of single units and tracking rhesus monkey behavior in an open field. The integrated system consists of four major components: (1) microelectrode assembly; (2) h

Measurement of the magnetic field inside the holes of a drilled bulk high-Tc superconductor

We use macroscopic holes drilled in a bulk YBCO superconductor to probe its magnetic properties in the volume of the sample. The sample is subjected to an AC magnetic flux with a density ranging from 30mT to 130mT and the flux in the superconductor is probed by miniature coils inserted in the holes. In a given hole, three different penetration regimes can be observed: (i) the shielded regime, where no magnetic flux threads the hole; (ii) the gradual penetration regime, where the waveform of the magnetic field has a clipped sine shape whose fundamental component scales with the applied field; and (iii) the flux concentration regime, where the waveform of the magnetic field is nearly a sine wave, with an amplitude exceeding that of the applied field by up to a factor of two. The distribution of the penetration regimes in the holes is compared with that of the magnetic flux density at the top and bottom surfaces of the sample, and is interpreted with the help of optical polarized light micrographs of these surfaces. We show that the measurement of the magnetic field inside the holes can be used as a local characterization of the bulk magnetic properties of the sample.