Disorder prediction methods, their applicability to different protein targets and their usefulness for guiding experimental studies

The role and function of a given protein is dependent on its structure. In recent years, however, numerous studies have highlighted the importance of unstructured, or disordered regions in governing a protein’s function. Disordered proteins have been found to play important roles in pivotal cellular functions, such as DNA binding and signalling cascades. Studying proteins with extended disordered regions is often problematic as they can be challenging to express, purify and crystallise. This means that interpretable experimental data on protein disorder is hard to generate. As a result, predictive computational tools have been developed with the aim of predicting the level and location of disorder within a protein. Currently, over 60 prediction servers exist, utilizing different methods for classifying disorder and different training sets. Here we review several good performing, publicly available prediction methods, comparing their application and discussing how disorder prediction servers can be used to aid the experimental solution of protein structure. The use of disorder prediction methods allows us to adopt a more targeted approach to experimental studies by accurately identifying the boundaries of ordered protein domains so that they may be investigated separately, thereby increasing the likelihood of their successful experimental solution.

Characteristics and properties of carboxymethylchitosan

Four samples of N,O-carboxymethylchitosan (0.5 < <(DS)over bar> < 1.5) were prepared by reacting chitosan (<(DA)over bar> = 24%) with monochloroacetic acid in the presence of excess sodium hydroxide. The carboxymethylchitosan samples were soluble in a wider range of pH as compared to the parent chitosan and the X-ray diffraction showed that they adopt a less ordered arrangement. The carboxymethylation of chitosan decreased the thermal stability of the polymer as evaluated by thermogravimetry but no clear dependence of the activation energy on the average degree of substitution of carboxymethylchitosan was identified. However, the values of activation energy of carboxymethylchitosan issued from the isothermal study depended on the degree of conversion, suggesting the occurrence of a complex set of simultaneous reactions. (C) 2008 Published by Elsevier Ltd.

Use of social network analysis to indentify the coopetition in a commercial cluster plan

The cluster provides a greater commercial relationship between the companies that comprise it. This encourages companies to adopt competitive structures that allow solving problems that would hardly alone (Lubeck et. Al., 2011). With that this paper aims to describe the coopetition between companies operating on a commercial cluster planned, from the point of view of retailers, taking as a basis the theoretical models proposed by Bengtsson and Kock (1999) and Leon (2005) and operationalized by means of Social Network Analysis (SNA). Data collection consisted of two phases, the first exploratory aspect to identify the actors, and the second was characterized as descriptive as it aims to describe the coopetition among the enterprises. As a result we identified the companies that cooperate and compete simultaneously (coopetition), firms that only compete, companies just cooperate and businesses that do not compete and do not cooperate (coexistence)

Seroprevalence of Antibodies to Chlamydophila psittaci in Zoo Workers in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Teaching the Rational Use of Medicines to medical students: a qualitative research

Background: Prescribing is a complex and challenging task that must be part of a logical deductive process based on accurate and objective information and not an automated action, without critical thinking or a response to commercial pressure. The objectives of this study were 1) develop and implement a discipline based on the WHO's Guide to Good Prescribing; 2) evaluate the course acceptance by students; 3) assess the impact that the Rational Use of Medicines (RUM) knowledge had on the students habits of prescribing medication in the University Hospital.Methods: In 2003, the RUM principal, based in the WHO's Guide to Good Prescribing, was included in the official curriculum of the Botucatu School of Medicine, Brazil, to be taught over a total of 24 hours to students in the 4th year. We analyzed the students' feedback forms about content and teaching methodology filled out immediately after the end of the discipline from 2003 to 2010. In 2010, the use of RUM by past students in their medical practice was assessed through a qualitative approach by a questionnaire with closed-ended rank scaling questions distributed at random and a single semistructured interview for content analysis.Results: The discipline teaches future prescribers to use a logical deductive process, based on accurate and objective information, to adopt strict criteria (efficacy, safety, convenience and cost) on selecting drugs and to write a complete prescription. At the end of it, most students considered the discipline very good due to the opportunity to reflect on different actions involved in the prescribing process and liked the teaching methodology. However, former students report that although they are aware of the RUM concepts they cannot regularly use this knowledge in their daily practice because they are not stimulated or even allowed to do so by neither older residents nor senior medical staff.Conclusions: This discipline is useful to teach RUM to medical students who become aware of the importance of this subject, but the assimilation of the RUM principles in the institution seems to be a long-term process which requires the involvement of a greater number of the academic members.

Using genetic algorithm to design protein sequence

In this work, genetic algorithms concepts along with a rotamer library for proteins side chains are used to optimize the tertiary structure of the hydrophobic core of Cytochrome b(562) starting from the known PDB structure of its backbone which is kept fixed while the side chains of the hydrophobic core are allowed to adopt the conformations present in the rotamer library. The atoms of the side chains forming the core interact via van der Waals energy. Besides the prediction of the native core structure, it is also suggested a set of different amino acid sequences for this core. Comparison between these new cores and the native are made in terms of their volumes, van der Waals energies values and the numbers of contacts made by the side chains forming the cores. This paper proves that genetic algorithms area efficient to design new sequence for the protein core. (C) 2007 Elsevier B.V. All rights reserved.

Influence of Wood Moisture Content on the Modulus of Elasticity in Compression Parallel to the Grain

Brazilian Standard ABNT NBR7190:1997 for timber structures design, adopts a first degree equation to describe the influence of wood moisture content. Periodically, when necessary, the referred standard is revised in order to analyze inconsistencies and to adopt considerations according new realities verified. So, the present paper aims to examine the adequacy of its equation which corrects to 12% of moisture the values of rigidity properties obtained on experimental tests. To quantify the moisture influence on modulus of elasticity, it was applied tests of compression parallel to the grain for six specimens of different strength classes, considering nominal moisture of 12; 20; 25; 30%. As results, modulus of elasticity in the moisture range 25-30% showed statistically equivalents, and was obtained a first degree equation to correlate the studied variables which leads to statically equivalent estimations when compared with results by ABNT NBR7190:1997 equation. However, it was indicated to maintain the current expression for the next text of the referred document review, without prejudice to statistical significance of the estimates.

Regulation, worker protection and active labour-market policies in Latin America

Includes bibliography

Contribution of energy services to the Millennium Development Goals and to poverty alleviation in Latin America and the Caribbean: executive summary

Includes bibliography

Contribution of energy services to the millennium development goals and to poverty alleviation in Latin America and the Caribbean

Includes bibliography

The international competitiveness of the Costa Rican clothing industry

Includes bibliography

Proposal on indicators for follow-up to the goals of the International Conference on Population and Development in Latin America and the Caribbean

Includes bibliography

Infrared to visible up-conversion emission in Er3+/Yb 3+ codoped fluoro-phosphate glass-ceramics

Erbium Er3+ and ytterbium Yb3+ codoped fluoro-phosphate glasses belonging to the system NaPO3-YF 3-BaF2-CaF2 have been prepared by the classical melt-quenching technique. Glasses containing up to 10 wt% of erbium and ytterbium fluorides have been obtained and characterized using differential scanning calorimetry (DSC) and UV-visible and near-infrared spectroscopy. Transparent and homogeneous glass-ceramics have been then reproducibly synthetized by appropriate heat treatment above glass transition temperature of a selected parent glass. Structural investigations of the crystallization performed through X-ray diffractometry (XRD) and scanning electron microscopy (SEM) have evidenced the formation of fluorite-type cubic crystals based during the devitrification process. Finally, infrared to visible up-conversion emission upon excitation at 975 nm has been studied on the Er3+ and Yb 3+ codoped glass-ceramics as a function of thermal treatment time. A large enhancement of intensity of the up-conversion emissions-about 150 times- has been observed in the glass-ceramics if compared to the parent glass one, suggesting an incorporation of the rare-earth ions (REI) into the crystalline phase. © 2012 The American Ceramic Society.

Ocorrência de anticorpos contra Salmonella e Mycoplasma em aves de criatórios de fundo de quintal próximos a explorações tecnificadas do Estado de São Paulo

Pós-graduação em Medicina Veterinária - FCAV

Towards an integrative approach to sustainability: exploring potential synergies between gender and environment

Includes bibliography.