1000 resultados para associação de técnicas multivariadas
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Pós-graduação em Medicina Veterinária - FCAV
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Neoplasias malignas da pele são os cênceres mais comuns da espécie humana. No entanto há tipos raros como o Carcinoma de células de Merkel (CCM), cuja incidência tem aumentado em todo mundo. O CCM possui curso agressivo, com freqüente envolvimento de nódulos linfáticos regionais e metástases à distância. Adicionalmente, afeta predominantemente idosos e imunocomprometidos, fato que levou-se a suspeita de uma possível etiologia infecciosa para essa neoplasia. Nesse foi-se isolado e descrito o MCPyV, um novo poliomavirus humano, diretamente de células tumorais do CCM. O objetivo do presente trabalho é dar continuidade à pesquisa desse novo vírus apresentando dados iniciais da pesquisa do MCPyV em número significativo de casos de CCM de pacientes brasileiros. Para tanto, foram analisadas 24 biópsias de CCM fixadas e incluídas em parafina, das quais foram extraído o material genômico e o produto submetido à PCR convencional com três pares de iniciadores descritos pela literatura (LT1, LT3 e VP1), com a finalidade de se detectar segmentos do vírus. No presente estudo o genoma viral foi detectado em 11/24 (45,8%) das amostras avaliadas, sendo que a positividade para cada par de iniciadores foi de 4/24 (16,7%) para LT1, 11/24 (45,8%) para LT3 e 4/24 (16.7%) para VP1. Essas freqüências são menores do que a relatada pela literatura e essa diferença pode ser devida a diferença nas amostras analisadas e nas técnicas empregadas. Outros estudos são necessários para comprovar a relação de causalidade, assim como desvendar o ciclo do MCPyV
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The Pirambóia Formation is a lithostratigraphic unit of the Paraná Basin, positioned between the Corumbataí (lower) and Botucatu (upper) Formations on the eastern edge of the basin. This unit is focused by many studies due to its great importance as an essential component in the Guarani Aquifer System (SAG) and the petroleum system “Irati-Pirambóia”, as excellent reservoirs. The Pirambóia Formation is historically the subject of several controversies on issues like age, contact relationships with the upper unit and depositional paleoenvironment. Despite these aspects, the Pirambóia Formation is commonly taken to be of Triassic age and is considered a product of wet aeolian systems, with plenty of wet interdunes and subordinate fluvial facies. In this work, by using techniques such as facies analysis, depositional architecture and facies association, facies of this unit were characterized and their depositional paleoenvironment was inferred particularly in Jundu Mining, region of Descalvado in northeastern São Paulo. Techniques such as grain size and petrographic analyses, aimed to characterize this unit as a potential reservoir rock. Five facies were described for the Pirambóia Formation in the studied region: St, Sh, Sm, Sr and Gt facies, generated by sedimentary processes of the bottom load type, mostly under low flow regime (with exception for the Sh facies, which is formed by upper flow regime processes). In addition to that, four facies associations were recognized from the architectural elements, primarily contained within the main channel: complex channel bars, composed by foreset macroforms (FM), sandy bedforms (SB) and gravel bars and bedforms (GB); flood deposits, constituted by laminated sand sheets (LS); deposits of hyperconcentrated flows and eolian deposits. It was interpreted that the Pirambóia Formation in Descalvado (SP) is the record of the sedimentation of braided rivers, with dunes and interdunes deposits...
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A análise estatística multivariada, extensão da análise univariada, consiste num conjunto de técnicas estatísticas, aplicadas quando há diversas variáveis relacionadas simultaneamente, sendo todas elas, em princípio, consideradas importantes no fenômeno em estudo. É de grande aplicação a conjuntos de dados das mais diversas áreas do conhecimento, principalmente da área biológica. Seu desenvolvimento teve um grande impulso na primeira metade do século passado. Entretanto, devido a complexidade dos cálculos matemáticos, principalmente envolvendo operações com matrizes de altas ordens, as aplicações somente se popularizaram nos dias atuais, com o desenvolvimento dos computadores e aplicativos computacionais. Técnicas estudadas: distâncias multivariadas, componentes principais, análise fatorial, correlações canônicas, análise de correspondência, teste t² de Hotelling, análise de variância multivariada (Manova), teste de normalidade multivariada, igualdade de matrizes de variâncias e covariâncias para populações multinormais
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Pós-graduação em Agronomia (Ciência do Solo) - FCAV
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Pós-graduação em Medicina Veterinária - FCAV
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Pós-graduação em Genética e Melhoramento Animal - FCAV
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This work aimed to evaluate some aspects of construction and operation of full-scale best management practices and their effects on infiltration loading rate. Two systems were studied: i) filter-swale-trench (FST) and ii) infiltration well (IW). In these units, field essays and full-scale measurements were made in order to assess the soil permeability. Further, the fine particles transportation and the geotextile blanket permeability were determined before and after operation. The results pointed out that there were soil transportation to inside the FST and IW systems, despite of the installed protections, and that these material were responsible for diminishing the geotextile (reduction from 30 to 90% for FST and 40 to 70% for IW) and the full-scale infiltration loading rates (varying from 4,7 x 10-6 to 10-5 m.s-1).
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This thesis presents the synthesis, characterization and study of the associative behaviour in aqueous media of new responsive graft copolymers, based on carboxymethylcellulose as the water-soluble backbone and Jeffamine® M-2070 e Jeffamine® M-600 (commercial polyetheramines) as the thermoresponsive grafts with high cloud point temperatures in water. The synthesis was performed on aqueous medium, by using 1-ethyl-3- (3-(dimethylamino)-propyl)carbodiimide hydrochloride and N-hydroxysuccinimide as activators of the reaction between carboxylategroupsfrom carboxymethylcellulose and amino groups from polyetheramines. The grafting reaction was confirmed by infrared spectroscopy and the grafting percentage by 1H NMR. The molar mass of the polyetheramines was determined by 1H NMR, whereas the molar mass of CMC and graft copolymers was determined by static light scattering. The salt effect on the association behaviour of the copolymers was evaluated in different aqueous media (Milli-Q water, 0.5M NaCl, 0.5M K2CO3 and synthetic sea water), at different temperatures, through UV-vis, rheology and dynamic light scattering. None of the copolymers solutions, at 5 g/L, turned turbid in Milli-Q water when heated from 25 to 95 °C, probably because of the increase in hydrophibicity promoted by CMC backbone. However, they became turbid in the presence of salts, due to the salting out effect, where the lowest cloud point was observed in 0.5M K2CO3, which was attributed to the highest ionic strength in water, combined to the ability of CO3 2- to decrease polymer-solvents interactions. The hydrodynamic radius and apparent viscosity of the copolymers in aqueous medium changed as a function of salts dissolved in the medium, temperature and copolymer composition. Thermothickening behaviour was observed in 0.5M K2CO3 when the temperature was raised from 25 to 60°C. This performance can be attributed to intermolecular associations as a physical network, since the temperature is above the cloud point of the copolymers in this solvent.
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Brazil is a country that is characterized by its low consumption of fish. With consumption records of 10.6 kg/ inhabitant/ year, it is lower than the recommended by the UN, that is 12 kg/ inhabitant/ year. The regular consumption of fish provides health gain for people and their introduction into the school feeding is an important strategy for the insertion of this food consumption habits in a population. In this context, the objective of this study was to understand the perception of fish with children from the public school system through the technical Projective Mapping (MP) and Association of Words (AP); and evaluate the acceptability of fish derivative in school meals. In the first instance with the intention to better understand the perception of children from different ages about the fish-based products, Projective Mapping techniques were applied through the use of food figures and word association. A total of 149 children from three public schools from Pato Branco, Paraná State, Brazil, took part in this study. Three groups of children aged 5-6, 7-8 and 9-10 years old were interviewed individually by six monitors experienced in applied sensory methods. Ten figures with healthy foods drawings (sushi, salad, fruit, fish, chicken), and less healthy foods (pizza, pudding, cake, hamburger, fries) were distributed to the children, who were asked to paste the figures in A3 sheet, so that the products they considered similar stayed near each other, and the ones considered very different stayed apart. After this, the children described the images and the image groups (Ultra Flash Profile). The results revealed that the MP technique was easily operated and understood by all the children and the use of images made its implementation easier. The results analysis also revealed different perceptions came from children from different ages and hedonic perceptions regarding the fish-based products had a greater weight in the percentage from older children. AP technique proved to be an important tool to understand the perception of fish by children, and strengthened the results previously obtained by the MP. In a second step it was evaluated the acceptance of fish burger (tilapia) in school meals. For this task, the school cooks were trained to prepare the hamburgers. For the evaluation of acceptance, the hedonic scale was used with 5 facial ratings (1 = disliked very much to 5 = liked a lot). Students from both genders, between 5 to 10 years old (n = 142) proved the burgers at lunchtime, representing the protein portion of the meal. The tilapia derivative products shown to be foods with important nutritional value and low calorie value. For the application of the multinomial logistic regression analysis there was no significant effect from the age and gender variation in the acceptance by children. However, statistical significance was determined in the interaction between these two variables. With 87 % acceptance rate there was potential for consumption of fish burgers in school meals.
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As associações culturais, como coletividades que emancipam o papel do cidadão na criação e produção de atividades culturais, contribuindo para a dinamização das sociedades, podem ser um veículo de desenvolvimento da educação artística, ao nível do ensino não formal e informal, tendo como ponto de partida o contributo da participação ativa dos cidadãos, que buscam a promoção e dinamização de atividades de teor cultural e artístico. Sendo assim, a seleção do Associativismo Cultural para o estudo da evolução da educação artística é um caminho para a compreensão da dinâmica das coletividades e o seu impacto no desenvolvimento cultural e artístico, a nível local, regional e nacional. Neste estudo, foi selecionada a Associação Cultural d´Orfeu, como estudo de caso, pela sua longevidade como associação e pelo seu destaque a nível cultural e artístico da zona centro do país. Ao nível da metodologia optámos por uma metodologia mista, tanto numa abordagem qualitativa, ao nível da análise de conteúdo, como quantitativa, na análise de dados. As técnicas de recolha de dados consistiram em entrevistas a dirigentes associativos e colaboradores (metodologia qualitativa), inquéritos a sócios, colaboradores e participantes de associações culturais (metodologia quantitativa), registos para diário de campo (acontecimentos, atividades e formação artística), análise de espólio documental da Associação Cultural d´Orfeu e registo audiovisual e fotográfico. Com este estudo, espera-se compreender as dinâmicas que estão inerentes ao associativismo cultural ao nível da educação/formação artística e que impactos apresentam no desenvolvimento cultural, social e artístico da sociedade. Além disso, queremos verificar que estratégias são utilizadas pelas associações culturais para a diversificação da oferta cultural e para o incentivo da cidadania ativa.
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La presente investigación tiene como objetivo establecer Tipologías Modales Multivariadas en Personalidad Eficaz en contextos de niños y niñas de 8 a 12 años chilenos y establecer las diferencias posibles en función de las variables edad, sexo y país (España y Chile). Como requisito previo se ha realizado una adaptacion del cuestionario de personalidad eficaz - primaria /8-12/es. En el capítulo I dedicado al marco conceptual y al planteamiento del objetivo del trabajo se entregan los antecedentes teóricos en relación al constructo de personalidad eficaz, formulado por Martín del Buey y Martín Palacio, sobre el que se viene realizando distintas investigaciones hace más de una década. Se hace una revisión de los antecedentes conceptuales en que se sustenta el constructo. Se aborda el concepto de el tipo modal multivariado, que permite la agrupación de los individuos en clases o tipos centrándose en la similitud de sus perfiles en base a más de un rasgo, destacando las técnicas de agrupamiento por conglomerados y los estudios realizados en torno a las Tipologías en Personalidad Eficaz. Para finalizar el capítulo I contempla una descripción de los cuestionarios de personalidad eficaz aplicados en Chile. En el capítulo II dedicado al campo empírico se presentan los análisis y resultados del estudio. Con una muestra formada por 2015 sujetos, 753 españoles (52.7% hombres y 47.3% mujeres) y 1262 chilenos (54.8% hombres y 45.2% mujeres). Todos ellos presentan edades comprendidas entre los 8 y 12 años. Se presentan los análisis psicométricos de fiabilidad, validez de estructura, además del análisis factorial confirmatorio análisis de diferencias en función de la varable edad, genero y país, los baremos en función de las diferencias encontradas. Y en base a estos resultados se presentan las tipologías obtenidas en contextos chilenos y se comparan con las obtenidas en contextos de igual edad y género españoles. Finalmente en el capítulo III se presentan las discusiones y conclusiones del estudio. Se finaliza con la presentación de las referencias bibliográficas utilizadas y los anexos correspondientes.
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MIPs are synthetic polymers that are used as biomimetic materials simulating the mechanism verified in natural entities such as antibodies and enzymes. Although MIPs have been successfully used as an outstanding tool for enhancing the selectivity or different analytical approaches, such as separation science and electrochemical and optical sensors, several parameters must be optimized during their synthesis. Therefore, the state-of-the-art of MIP production as well as the different polymerization methods are discussed. The potential selectivity of MIPs in the extraction and separation techniques focusing mainly on environmental, clinical and pharmaceutical samples as applications for analytical purposes is presented.