Enzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Harpalycin 2 inhibits the enzymatic and platelet aggregation activities of PrTX-III, a D49 phospholipase A(2) from Bothrops pirajai venom

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Triterpene benzoates from the bark of Picramnia teapensis (Simaroubaceae)

Two new benzoic acid esters of triterpene alcohols [lup-20 (29)-en-28-oic acid 3 alpha, 7 beta -dibenzoate and 3 alpha -hydroxy-lup-20(29)-en-28-ic acid 7 beta -benzoate] were isolated from the stem bark of Picramnia teapensis Tul. The structures of these compounds were established on the basis of spectral analyses. Other known compounds, beta -sitosterol, estigmasterol, lupeol and epilupeol, were identified in mixture by GC-MS. The triterpene esters have not shown in-vitro inhibitory effect on the growth of Leucoagaricus gongylophorus (Fisher), referred also as Leucocoprinus gongylophorus (Heim), syn Rozites gongylophora (Moller), the symbiotic fungus cultivated by the leaf-cutting ant Atta sexdens L.

Desempenho de Lecanicillium lecanii em meios de cultura contendo vitaminas e concentrações de extrato de levedura

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos de subprodutos da fotodegradação da deltametrina na população de Tetranychus urticae Koch (Acari: Tetranychidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Synthesis, characterization and thermal behaviour of solid-state compounds of benzoates with some bivalent transition metal ions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cytotoxicity of monomers, plasticizer and degradation by-products released from dental hard chairside reline resins

Objectives. The aim of this study was to evaluate the cytotoxic effect of the monomers isobutyl methacrylate (IBMA) and 1,6-hexanediol dimethacrylate (1,6-HDMA), the plasticizer di-n-butyl phthalate (DBP), and the degradation by-products methacrylic acid (MA) and benzoic acid (BA) on L929 cells. Based on previous investigations on the release of these compounds from hard chairside reline resins, a range of concentrations (mu mol/L) were selected for the cytotoxicity tests (IBMA, 5.491406.57; 1,6-HDMA, 1.2239.32; DBP, 1.12143.8; MA, 9.07581; BA, 3.19409).Methods. Cytotoxic effects were assessed using MTT and 3H-thymidine assays after the cells had been exposed to the test compounds at the given concentrations for 24h. Cytotoxicity was rated based on cell viability relative to controls (cells exposed to medium without test substances).Results. DNA synthesis activity was inhibited by all compounds. Mitochondrial dehydrogenase activity decreased in cells treated with monomers, plasticizer and MA by-product, whereas no cytotoxic effect was observed on contact with BA at the majority of concentrations tested. The ranges of suppression for 3H-thymidine assay were: IBMA, 2595%; 1,6-HDMA, 9598%; DBP, 4098%; MA, 9799%; BA, 5471%. For MTT assay, the ranges of suppression were: IBMA, 096%; 1,6-HDMA, 2689%; DBP, 1780%; MA, 5266%; BA, 027%. The 3H-thymidine assay was more sensitive than the MTT assay.Significance. This study evaluated the cytotoxicity of a wide range of concentrations of monomers (IBMA and 1,6-HDMA), plasticizer (DBP) and degradation by-products (MA and BA), including those expected to be released from hard chairside reline resins. The differences observed in the cytotoxicity of these compounds, along with other properties, may assist the dental practitioners in the selection of reline materials with improved service life performance and low risk of adverse reactions in patients who wear relined dentures.

Leachability of degradation products from hard chairside reline resins in artificial saliva: effect of water-bath post-polymerization treatment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alkamides and phenethyl derivatives from Aristolochia gehrtii

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Neolignans, styrylpyrones and flavonoids from an Aniba species

The trunk wood and barks from an Aniba species contain four esters of benzoic acid with cinnamyl alcohol, five benzofuran neolignans, licarin-A, burchellin, cis-burchellin, burchellin-rearranged and cis-burchellin-rearranged, one tetrahydrofuran neolignan, aristolignin, three bicyclooctane guianin-type neolignans, (7S, 8S, 1'R, 5'R)-4-hydroxy-3,3'-dimethoxy-4',6'-dioxo-8.1', 7.5'-neolignan-Delta: 1,3,5,2',8' and the new (7S, 8S, 1'R, 4'R, 5'S)-4'-hydroxy-3,4,3'-trimethoxy-6'-oxo-8.1', 7.5'-neolignan-Delta: 1,3,5,2',8' and (7S, 8S, 1'R, 4'R, 5'S)-4,4'-dihydroxy-3,3'-dimethoxy-6'-oxo-8.1', 7.5'-neolignan-Delta: 1,3,5,2',8', one new bicyclooctane canellin-type neolignan (7S, 8S, 1'S, 4'R, 5'R, 6'S)-4',6'-dihydroxy-3,4-dimethoxy-3'-oxo-8.1', 7.5'-neolignan-Delta: 1,3,5,8', two styrylpyrones, 4-methoxy-6-(11,12-dimethoxy-trans-styryl)-2-pyrone and 6-(11,12-methylenedioxy-trans-styryl)-4-methoxy-2-pyrone, two styrylpyrone dimers: 4'-methoxy-8-(11,12-dimethoxyphenyl)-7-[6-(4-methoxy-2-pyronyl)-6-(E)-styryl-1'-oxabicyclo[4,2,0]octa-4'-en-2'-one and the new 11,12-dimethoxyphenyl-7,7'-di-[6-(4-methoxy-2-pyronyl)]-cyclobutane and six flavonoids, 3,5-dihydroxy-7,4'-dimethoxyflavone, 5-hydroxy-3,7,4'-trimethoxyflavone, 3,5,4'-trihydroxy-7-methoxyflavone, 2,3-dihydro-5-hydroxy-7,4'-dimethoxyflavone, 2,3-dihydro-3,5-dihydroxy-7-methoxyflavone, 2,3-dihydro-3,5-dihydroxy-7,4'-dimethoxyflavone and a new flavan, 6,7,3',4',5'-pentamethoxyflavan. (C) 1997 Elsevier B.V. Ltd.

Chemistry and evolution of the Piperaceae

The chemistry of members of the family Piperaceae is of great interest owing to the variety of biological properties displayed. A survey of structural diversity and bioactivity reveals that groups of species specialize in the production of amides, phenylpropanoids, lignans and neolignans, benzoic acids and chromenes, alkaloids, polyketides, and a plethora of compounds of mixed biosynthetic origin. Bioassays against Cladosporium cladosporioides and C. sphaerospermun have resulted in the characterization of various amides, prenylated phenolic compounds, and polyketides as potential classes of antifungal agents. Studies on the developmental process in seedlings of Piper solmsianum have shown that phenylpropanoid are produced instead of the tetrahydrofuran lignans found in adult plants. In suspension cultures of P. cernuum and P crassinervium, phenylethylamines and alkamides predominate, whereas in the adult plants prenylpropanoids and prenylated benzoic acids are the respective major compound classes. Knowledge of the chemistry, bioactivity, and ecology of Piperaceae species provides preliminary clues for an overall interpretation of the possible role and occurrence of major classes of compounds.

Phenolics from Brazilian propolis

The main phenolic constituents from Brazilian propolis, originating from Sao Paulo State, were isolated and identified: three flavonoids, a prenylated coumaric acid and two new benzopyranes, E and Z 2,2-dimethyl-6-carboxyethenyl-8-prenyl-2H-benzopyranes.

On the application of nanostructured electrodes prepared by Ti/TiO2/WO3 template : A case study of removing toxicity of indigo using visible irradiation

New assays with HepG2 cells indicate that Indigo Carmine (IC), a dye that is widely used as additive in many food and pharmaceutical industries exhibited cytotoxic effects. This work describes the development of a bicomponent nanostructured Ti/TiO2/WO3 electrode prepared by template method and investigates its efficiency in a photoelectrocatalytic method by using visible light irradiation and applied potential of 1V. After 2h of treatment there are reduction of 97% discoloration, 62% of mineralization and formation of three byproducts assigned as: 2-amine-5-sulfo-benzoic acid, 2,3-dioxo-14-indole-5-sulfonic acid, and 2-amino-α-oxo-5-sulfo-benzeneacetic acid were identified by HPLC-MS/MS. But, cytotoxicity was completely removed after 120min of treatment. © 2013 Elsevier Ltd.

Rapid determination of furosemide by combined spot test/diffuse reflectance spectroscopy to detect doping in sport

This paper describes the development and application of a simple, cheap, and clean method for the quantification of furosemide in urine samples from athletes, to detect doping, using a combined spot test/diffuse reflectance spectroscopy procedure. The method is based on the complexation reaction of furosemide (5-(aminosulfonyl)-4-chloro-2-((furanylmethyl)amino)benzoic acid, dissolved in ethanol, with FeCl3 and the surfactant dodecyltrimethylammonium bromide (DTAB) in aqueous solution, yielding a colored compound on the surface of a filter paper. The reagent concentrations were optimized using a chemometric experimental design. The reflectometric measurements of the complex formed were carried out at 477nm. The linear range obtained was 1.65-9.00×10-3molL-1 of furosemide (R=0.997), and the detection and quantification limits were 4.9×10-4 and 1.62×10-3molL-1, respectively. The proposed method was successfully applied in the analysis of furosemide in spiked urine, demonstrating that it is a reliable alternative method for the detection of furosemide doping in sport. © 2012 Elsevier B.V..

Nanopartículas de sílica e ácido p-amino benzóico: um estudo de associação para protetores solares

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)