A hispanical dimension of the luso-american revolution (1820-1823)

The Independence of Brazil was proclaimed in September 1822 after two years during which constituent deputies elected on both sides of the Atlantic and gathered in Lisbon endeavoured to achieve unification with the former Portuguese metropoli. Due to the failure of these attempts a Constituent Assembly settled in independent Brazil in July 1823. Those two parlamentary experiences - the first to take place in the Portuguese dominions - were strongly influenced by the decisions adopted in Cadiz (1810-1814), and in Madrid (1820-1823), and by the independentist processes developed at the time in Spanish America. This work aims at observing that influence on the decisions adopted in Lisbon and Rio de Janeiro vis-a-vis the autonomical reivindications of several Brazilian provinces.

Rational combinatorial chemistry-based selection, synthesis and evaluation of an affinity adsorbent for recombinant human clotting factor VII

The selection, synthesis and chromatographic evaluation of a synthetic affinity adsorbent for human recombinant factor VIIa is described. The requirement for a metal ion-dependent immunoadsorbent step in the purification of the recombinant human clotting factor, FVIIa, has been obviated by using the X-ray crystallographic structure of the complex of tissue factor (TF) and Factor VIIa and has directed our combinatorial approach to select, synthesise and evaluate a rationally-selected affinity adsorbent from a limited library of putative ligands. The selected and optimised ligand comprises a triazine scaffold bis-substituted with 3-aminobenzoic acid and has been shown to bind selectively to FVIIa in a Ca2+-dependent manner. The adsorbent purifies FVIIa to almost identical purity (>99%), yield (99%), activation/degradation profile and impurity content (∼1000 ppm) as the current immunoadsorption process, while displaying a 10-fold higher static capacity and substantially higher reusability and durability. © 2002 Elsevier Science B.V. All rights reserved.

Pope Francis and the challenges of inter-civilization diplomacy

Abstract This article addresses the role of Pope Francis in the interfaith and inter-civilization dialogue of the Holy See. Specifically, it analyses the main aspects of diplomatic relations between the Holy See and Russia, China, Islamic countries and Israel, and provides perspectives on the inter-civilization diplomacy of the Holy See during the current pontificate.

Aspectos ecológicos da tripanossomíase americana: VII. Permanência e mobilidade do Triatoma sordida em relação aos ecótopos artificiais

Nas colônias desenvolvidas espontaneamente em ecótopos artificiais, representados por galinheiros experimentais, procurou-se observar a permanência e a mobilidade de Triatoma sordida. Para tanto, procedeu-se à marcação individual de adultos e ninfas de 4.° e 5.° estádios, bem como à instalação de abrigos ao redor daqueles biótopos. Os dados obtidos permitiram observar a curta permanência dos primeiros na colônia, não ultrapassando o período de dois meses para a maioria dessas formas. A mobilidade evidenciou-se pela presença de ovos e ninfas de 1.° estádio, nos esconderijos instalados, e as evidências sugeriram ser devidas à passagem dos adultos por esses ecótopos. Concluiu-se que esta espécie é dotada de elevada capacidade de disseminação, em concordância com sua apreciável valência ecológica.

Estudo das condições de saúde das crianças do Município de São Paulo (Brasil), 1984/1985: VII - Parasitoses intestinais

Como parte de estudo populacional sobre condições de saúde na infância, uma amostra representativa das crianças menores de cinco anos residentes no Município de São Paulo, SP (Brasil) (n=695) foi submetida a exames parasitológicos de fezes. Os exames foram realizados através da técnica de sedimentação e, quando as fezes tinham consistência amolecida ou liquefeita, também pela técnica do exame direto. A prevalência de enteroparasitoses em geral foi de 30,9%, sendo de 16,4%, 14,5% e 12,5% as prevalências específicas da ascaridíase, giardíase e tricuríase. Prevalências inferiores foram assinaladas para os enteroparasitas E. histolytica, H. nana e S. stercoralis, respectivamente 2,0%, 0,9% e 0,3%. Em apenas uma criança foram encontrados ovos de ancilostomídeos e em nenhuma delas ovos de Schistosoma mansoni. Das crianças examinadas, 13,1% apresentaram duas ou mais espécies de enteroparasitas e 4,8% três ou mais. As prevalências atuais, comparadas às prevalências encontradas em 1973/74 por outro inquérito populacional realizado no município, indicam queda expressiva da ascaridíase e tricuríase, mas não da giardíase. A estratificação das prevalências segundo faixa etária revelou aumento significativo com a idade da criança, chamando atenção o aumento que ocorre do primeiro para o segundo ano de vida. As enteroparasitoses aumentam também significativamente sua freqüência à medida que piora o nível socioeconômico, chegando a ser de nove vezes a diferença de prevalência existente entre os estratos socioeconômicos extremos da população. No caso específico da giardíase o gradiente socioeconômico foi consideravelmente menor do que o encontrado para as demais enteroparasitoses, o que confirma a maior complexidade epidemiológica do problema.

Disruption and phenotypic analysis of six open reading frames from chromosome VII of Saccharomyces cerevisiae reveals one essential gene

Six open reading frames (ORFs) located on chromosome VII of Saccharomyces cerevisiae (YGR205w, YGR210c, YGR211w, YGR241c, YGR243w and YGR244c) were disrupted in two different genetic backgrounds using short-flanking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YGR211w, recently identified as the yeast ZPR1 gene, is an essential gene. The other five genes are non-essential, and no phenotypes could be associated to their inactivation. Two of these genes have recently been further characterized: YGR241c (YAP1802) encodes a yeast adaptor protein and YGR244c (LSC2) encodes the b-subunit of the succinyl-CoA ligase. For each ORF, a replacement cassette with long flanking regions homologous to the target locus was cloned in pUG7, and the cognate wild-type gene was cloned in pRS416.

Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes

We report the nucleotide sequence of a 17,893 bp DNA segment from the right arm of Saccharomyces cerevisiae chromosome VII. This fragment begins at 482 kb from the centromere. The sequence includes the BRF1 gene, encoding TFIIIB70, the 5' portion of the GCN5 gene, an open reading frame (ORF) previously identified as ORF MGA1, whose translation product shows similarity to heat-shock transcription factors and five new ORFs. Among these, YGR250 encodes a polypeptide that harbours a domain present in several polyA binding proteins. YGR245 is similar to a putative Schizosaccharomyces pombe gene, YGR248 shows significant similarity with three ORFs of S. cerevisiae situated on different chromosomes, while the remaining two ORFs, YGR247 and YGR251, do not show significant similarity to sequences present in databases.

Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII

A 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduced amino acid sequence (and their physical arrangement is similar to that) of the YHR161c and YHR162w ORFs from chromosome VIII.

Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs.

A 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722 with the YAL023 (PMT2) and PMT1 genes, encoding two integral membrane proteins, and G7727 with the first half of the genes encoding elongation factors 1gamma, TEF3 and TEF4. Two other ORFs, G7742 and G7744, are most probably yeast orthologues of the human and Paracoccus denitrificans electron-transferring flavoproteins (beta chain) and of the Escherichia coli phosphoserine phosphohydrolase. The five remaining identified ORFs do not show detectable homology with other protein sequences deposited in data banks. The sequence has been deposited in the EMBL data library under Accession Number Z49133.

The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames

We report the sequence of a 9000 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII. Analysis of the sequence revealed four complete previously unknown open reading frames, which were named G7587, G7589, G7591 and G7594 following standard rules for provisional nomenclature. Outstanding features of some of these proteins were the homology of the putative protein coded by G7589 with proteins involved in transcription regulation and the transmembrane domains predicted in the putative protein coded by G7591.