996 resultados para Observation en direct


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Mode of access: Internet.

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Fil: Daule, Gabriela. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

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We evaluated the role of the larval parasitoid, Diadegma semiclausum Hellen (Hymenoptera: Ichneumonidae), in controlling Plutella xylostella (L.) (Lepidoptera: Plutellidae) by cage exclusion experiments and direct field observation during the winter season in southern Queensland, Australia. The cage exclusion experiment involved uncaged, open cage and closed cage treatments. A higher percentage (54-83%) of P. xylostella larvae on sentinel plants were lost in the uncaged treatment than the closed (4-9%) or open cage treatments (11-29%). Of the larvae that remained in the uncaged treatment, 72-94% were parasitized by D. semiclausum , much higher than that in the open cage treatment (8-37% in first trial, and 38-63% in second trial). Direct observations showed a significant aggregation response of the field D. semiclausum populations to high host density plants in an experimental plot and to high host density plots that were artificially set-up near to the parasitoid source fields. The degree of aggregation varied in response to habitat quality of the parasitoid source field and scales of the manipulated host patches. As a result, density-dependence in the pattern of parasitism may depend on the relative degree of aggregation of the parasitoid population at a particular scale. A high degree of aggregation seems to be necessary to generate density-dependent parasitism by D. semiclausum . Integration of the cage exclusion experiment and direct observation demonstrated the active and dominant role of this parasitoid in controlling P. xylostella in the winter season. A biologically based IPM strategy, which incorporates the use of D. semiclausum with Bt, is suggested for the management of P. xylostella in seasons or regions with a mild temperature.

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The interactions of the unpaired thiol residue (Cys34) of human serum albumin (HSA) with low-molecular-weight thiols and an Au(I)-based antiarthritic drug have been examined using electrospray ionization mass spectrometry. Early measurements of the amount of HSA containing Cys34 as the free thiol suggested that up to 30% of circulating HSA bound cysteine as a mixed disulfide. It has also been suggested that reaction of HSA with cysteine, occurs only on handling and storage of plasma. In our experiments, there were three components of HSA in freshly collected plasma from normal volunteers, HSA, HSA + cysteine, and HSA + glucose in the ratio similar to50:25:25. We addressed this controversy by using iodoacetamide to block the free thiol of HSA in fresh plasma, preventing its reaction with plasma cysteine. When iodoacetamide was injected into a vacutaner tube as blood was collected, the HSA was modified by iodoacetamide, with 20-30% present as the mixed disulfide with cysteine (HSA + cys). These data provide strong evidence that 20-30% of HSA in normal plasma contains one bound cysteine. Reaction of HSA with [Au(S2O3)(2)](3-) resulted in formation of the adducts HSA + Au(S2O3) and HSA + Au. Reaction of HSA with iodoacetamide prior to treatment with [Au(S2O3)(2)](3-) blocked the formation of gold adducts. (C) 2003 Elsevier Inc. All rights reserved.

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A magnetic difference neutron diffraction study of a rare-earth (Tb) phosphate glass has revealed exclusively the Tb...Tb distances. The difference between data taken with and without an applied magnetic field of 4 T shows Tb...Tb pairwise atomic correlations at 3.9 and 6.4 A, respectively, with relative coordination numbers of 1:14. The first distance arises when two Tb3+ ions share a common oxygen neighbor, and indicates a clustering of rare-earth ions. The second distance arises when two Tb3+ ions are coordinated to different oxygens in the same PO4 group, in a near-linear arrangement.

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Fast X-ray photoelectron spectroscopy reveals that the efficient catalytic destruction of 1,1,1-trichloroethane occurs over Pt{111} surfaces at temperatures as low as 150 K. Decomposition occurs via rapid, sequential C-Cl bond scission to form an alkylidyne surface intermediate that in turn dehydrogenates above room temperature. Atomic chlorine liberated during dehydrochlorination undergoes efficient reaction with surface hydrogen, resulting in the evolution of gaseous HCl and small amounts of ethane, presumably via ethylidyne hydrogenation. Irreversible dehydrogenation of residual hydrocarbon fragments results in significant surface coking above 500 K.

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We present results of the direct observation, in real-space, of the phase separation of high molecular weight polystyrene and poly(methyl methacrylate) from ortho-xylene using our newly developed technique of high speed stroboscopic interference microscopy. Taking a fixed concentration (3 wt % in o-xylene) at a fixed composition (1:4 by weight) and by varying the rotational rate during the spin-coating process, we are able to observe the formation of a range of phase separated bicontinuous morphologies of differing length-scales. Importantly, we are able to show that the mechanism by which the final phase separated structure is formed is through domain coarsening when rich in solvent, before vitrification occurs and fixes the phase separated structure. The ability to directly observe morphological development offers a route toward controlling the length-scale of the final morphology through process control and in situ feedback, from a single stock solution. © 2013 Wiley Periodicals, Inc.

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El objetivo de este artículo es presentar las observaciones realizadas durante la consulta de los originales de los códices Borgia (Borg. Mess. 1) y Vaticano B (Vat. Lat. 3773) en la Biblioteca Apostólica Vaticana en 2014. Este estudio codicológico, aunque a la fuerza parcial, dado que fue realizado solamente por medio de una observación directa, revela nuevos datos acerca de la elaboración de los manuscritos prehispánicos, tanto en la preparación de la piel como soporte como en los procedimientos aplicados a la hora de trazar las imágenes y corregir las equivocaciones.

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L'étude a pour objet la vérification du lien entre la participation d’un programme d'entraînement aux habiletés sociales en présence d'agents de changement et le répertoire d'habiletés sociales. Les hypothèses soutiennent que le répertoire augmentera suite à l'entraînement et que le groupe assisté des parents aura une augmentation significativement supérieure à celui assisté des pairs. Une étude quasi expérimentale impliquant huit sujets permet, au moyen d'une observation systématique avant et après l'entraînement, d'évaluer le répertoire d'habiletés sociales et de faire une comparaison entre les trois groupes. Parmi ceux-ci, l'un est accompagné des parents, l'un est accompagné des pairs et l'autre constitue le groupe contrôle, c'est-à-dire qu'il ne participe pas à l'intervention. Les résultats montrent que le lien entre la participation à un entraînement aux habiletés sociales et la modification du répertoire d'habiletés sociales n'est pas significatif. Ils montrent également qu'il n'est pas significatif entre le type d'agent de changement utilisé (parents ou pairs) et ce même répertoire. L'étude révèle l'importance de continuer à explorer le champ des habiletés sociales en réexaminant le type d'évaluation. En effet, les grilles d'observation devraient avoir un lien direct et indiscutable avec les programmes d'entraînement mis en application. De plus, un nombre supérieur de sujets permettrait une analyse statistique plus rigoureuse.