Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects

Evolving levels of resistance in insects to the bioinsecticide Bacillus thuringiensis (Bt) can be dramatically reduced through the genetic engineering of chloroplasts in plants. When transgenic tobacco leaves expressing Cry2Aa2 protoxin in chloroplasts were fed to susceptible, Cry1A-resistant (20,000- to 40,000-fold) and Cry2Aa2-resistant (330- to 393-fold) tobacco budworm Heliothis virescens, cotton bollworm Helicoverpa zea, and the beet armyworm Spodoptera exigua, 100% mortality was observed against all insect species and strains. Cry2Aa2 was chosen for this study because of its toxicity to many economically important insect pests, relatively low levels of cross-resistance against Cry1A-resistant insects, and its expression as a protoxin instead of a toxin because of its relatively small size (65 kDa). Southern blot analysis confirmed stable integration of cry2Aa2 into all of the chloroplast genomes (5,000–10,000 copies per cell) of transgenic plants. Transformed tobacco leaves expressed Cry2Aa2 protoxin at levels between 2% and 3% of total soluble protein, 20- to 30-fold higher levels than current commercial nuclear transgenic plants. These results suggest that plants expressing high levels of a nonhomologous Bt protein should be able to overcome or at the very least, significantly delay, broad spectrum Bt-resistance development in the field.

The Imperial gazetteer of India /

Reprint of the 1907-1909 ed. published by Clarendon Press, London ; 1st ed., 9 v., 1881, and 2d ed., 14 v., 1885-1887, edited by Sir William Wilson Hunter.

A Global Review on Peri-Urban Development and Planning

Urban regions worldwide are increasingly facing the challenge of dealing with highly dynamic metropolitan growth and, at the same time, institutional changes like decentralisation and globalisation. These kinds of changes express themselves most evidently in peri-urban areas, where urban and rural life meets. These peri-urban areas in particular have been the stage for rapid physical, social and economic transformations, both in developed and developing countries. Peri-urbanization takes place here. Based on literature review, this paper presents an effort to identify generic attributes of peri-urbanisation and the way in which development planning tends to reply. Three major attributes are identified: peri-urban space (the spatial expression of peri-urban development), peri-urban life (the functional appearance of land uses, activities and peri-urban innovation), and peri-urban change (a causal and temporal perspective featuring flows and drivers of change). It is also shown that prevalent institutional replies in planning and development generally fail to acknowledge the dynamic and increasingly fragmented attributes of global peri-urbanisation.

Systematic review of Late Jurassic sauropods from the Museu Geológico collections (Lisboa, Portugal)

The Museu Geológico collections house some of the first sauropod references of the Lusitanian Basin Upper Jurassic record, including the Lourinhasaurus alenquerensis and Lusotitan atalaiensis lectotypes, previously considered as new species of the Apatosaurus and Brachiosaurus genera, respectively. Several fragmentary specimens have been classical referred to those taxa, but the most part of these systematic attributions are not supported herein, excluding a caudal vertebra from Maceira (MG 8804) considered as cf. Lusotitan atalaiensis. From the material housed in the Museu Geológico were identified basal eusauropods (indeterminate eusauropods and turiasaurs) and neosauropods (indeterminate neosauropods, diplodods and camarasaurids and basal titanosauriforms). Middle caudal vertebrae with lateral fossae, ventral hollow border by pronounced ventrolateral crests and quadrangular cross-section suggest for the presence of diplodocine diplodocids in north area of the Lusitanian Basin Central Sector during the Late Jurassic. A humerus collected from Praia dos Frades (MG 4976) is attributed to cf. Duriatitan humerocristatus suggesting the presence of shared sauropod forms between the Portugal and United Kingdom during the Late Jurassic. Duriatitan is an indeterminate member of Eusauropoda and the discovery of new material in both territories is necessary to confirm this systematic approach. The studied material is in according with the previous recorded paleobiodiversity for the sauropod clade during the Portuguese Late Jurassic, which includes basal eusauropods (including turiasaurs), diplodocids and macronarians (including camarasaurids and basal titanosauriforms).

Tietokantaratkaisuehdotus pientasajännitesähköverkoston mittausdatavarastoksi

Lappeenrannan teknillinen yliopisto tutkii pientasajännitesähkön käyttöä. Yliopisto on rakennuttanut Järvi-Suomen Energia Oy:n ja Suur-Savon Sähkö Oy:n kanssa yhteistyössä kokeellisen pientasajännitesähköverkon, jolla pystytään tarjoamaan kenttäolosuhteet pienjännitetutkimukselle todellisilla asiakkailla ja todentaa LVDC-teknologiaa ja muita älykkään sähköverkon toimintoja kenttäolosuhteissa. Verkon tasajänniteyhteys on rakennettu 20 kV sähkönjakeluverkon ja neljän kuluttajan välille. 20 kV keskijännite suunnataan tasamuuntamolla ±750 V pientasajännitteeksi ja uudestaan 400/230 V vaihtojännitteeksi kuluttajien läheisyydessä. Tämän kandidaatintyön tarkoituksena on luoda yliopistolle tietokanta pientasajännitesähköverkosta kertyvälle tiedolle ja mittaustuloksille. Tietokanta nähtiin tarpeelliseksi luoda, jotta pienjänniteverkon mittaustuloksia pystytään myöhemmin tarkastelemaan yhdessä ja yhtenäisessä muodossa. Yhdeksi tutkimuskysymykseksi muodostui, kuinka järjestää ja visualisoida kaikki verkosta palvelimille kertyvä mittausdata. Työssä on huomioitu myös kolme tietokantaa mahdollisesti hyödyntävää käyttäjäryhmää: kotitalousasiakkaat, sähköverkkoyhtiöt ja tutkimuslaboratorio, sekä pohdittu tietokannan hyötyä ja merkitystä näille käyttäjille. Toiseksi tutkimuskysymykseksi muodostuikin, mikä kaikesta tietokantaan talletetusta datasta olisi oleellisen tärkeää ottaa talteen näiden asiakkaiden kannalta, ja kuinka nämä voisivat hakea tietoa tietokannasta. Työn tutkimusmenetelmät perustuvat jo valmiiksi olemassa olevaan mittausdataan. Työtä varten on käytetty sekä painettua että sähköisessä muodossa olevaa kirjallisuutta. Työn tuloksena on saatu luotua tietokanta MySQL Workbench -ohjelmistolla, sekä mittausdatan keräys- ja käsittelyohjelmat Python-ohjelmointikielellä. Lisäksi on luotu erillinen MATLAB-rajapinta tiedon visualisoimista varten, jolla havainnollistetaan kolmen asiakasryhmän mittausdataa. Tietokanta ja sen tiedon visualisointi antavat kuluttajalle mahdollisuuden ymmärtää paremmin omaa sähkönkäyttöään, sekä sähköverkkoyhtiöille ja tutkimuslaboratorioille muun muassa tietoa sähkön laadusta ja verkon kuormituksesta.

Nd-Sr isotopes, diatom and biogenic opal content of Pliocene sediments from IODP Site 318-U1361

Warm intervals within the Pliocene epoch (5.33-2.58 million years ago) were characterized by global temperatures comparable to those predicted for the end of this century (Haywood and Valdes, doi:10.1016/S0012-821X(03)00685-X) and atmospheric CO2 concentrations similar to today (Seki et al., 2010, doi:10.1016/j.epsl.2010.01.037; Bartoli et al., 2011, doi:10.1029/2010PA002055; Pagani et al., 2010, doi:10.1038/ngeo724). Estimates for global sea level highstands during these times (Miller et al., 2012, doi:10.1130/G32869.1) imply possible retreat of the East Antarctic ice sheet, but ice-proximal evidence from the Antarctic margin is scarce. Here we present new data from Pliocene marine sediments recovered offshore of Adélie Land, East Antarctica, that reveal dynamic behaviour of the East Antarctic ice sheet in the vicinity of the low-lying Wilkes Subglacial Basin during times of past climatic warmth. Sedimentary sequences deposited between 5.3 and 3.3 million years ago indicate increases in Southern Ocean surface water productivity, associated with elevated circum-Antarctic temperatures. The geochemical provenance of detrital material deposited during these warm intervals suggests active erosion of continental bedrock from within the Wilkes Subglacial Basin, an area today buried beneath the East Antarctic ice sheet. We interpret this erosion to be associated with retreat of the ice sheet margin several hundreds of kilometres inland and conclude that the East Antarctic ice sheet was sensitive to climatic warmth during the Pliocene.