The final N-terminal trimming of a subaminoterminal proline-containing HLA class I-restricted antigenic peptide in the cytosol is mediated by two peptidases.

The proteasome produces MHC class I-restricted antigenic peptides carrying N-terminal extensions, which are trimmed by other peptidases in the cytosol or within the endoplasmic reticulum. In this study, we show that the N-terminal editing of an antigenic peptide with a predicted low TAP affinity can occur in the cytosol. Using proteomics, we identified two cytosolic peptidases, tripeptidyl peptidase II and puromycin-sensitive aminopeptidase, that trimmed the N-terminal extensions of the precursors produced by the proteasome, and led to a transient enrichment of the final antigenic peptide. These peptidases acted either sequentially or redundantly, depending on the extension remaining at the N terminus of the peptides released from the proteasome. Inhibition of these peptidases abolished the CTL-mediated recognition of Ag-expressing cells. Although we observed some proteolytic activity in fractions enriched in endoplasmic reticulum, it could not compensate for the loss of tripeptidyl peptidase II/puromycin-sensitive aminopeptidase activities.

1H-[13C] NMR spectroscopy of the rat brain during infusion of [2-13C] acetate at 14.1 T.

Full signal intensity (1)H-[(13)C] NMR spectroscopy, combining a preceding (13)C-editing block based on an inversion BISEP (B(1)-insensitive spectral editing pulse) with a spin-echo coherence-based localization, was developed and implemented at 14.1 T. (13)C editing of the proposed scheme was achieved by turning on and off the (13)C adiabatic full passage in the (13)C-editing block to prepare inverted and noninverted (13)C-coupled (1)H coherences along the longitudinal axis prior to localization. The novel (1)H-[(13)C] NMR approach was applied in vivo under infusion of the glia-specific substrate [2-(13)C] acetate. Besides a approximately 50% improvement in sensitivity, spectral dispersion was enhanced at 14.1 T, especially for J-coupled metabolites such as glutamate and glutamine. A more distinct spectral structure at 1.9-2.2 ppm(parts per million) was observed, e.g., glutamate C3 showed a doublet pattern in both simulated (1)H spectrum and in vivo (13)C-edited (1)H NMR spectra. Besides (13)C time courses of glutamate C4 and glutamine C4, the time courses of glutamate C3 and glutamine C3 obtained by (1)H-[(13)C] NMR spectroscopy were reported for the first time. Such capability should greatly improve the ability to study neuron-glial metabolism using (1)H-observed (13)C-edited NMR spectroscopy.

Escriure (correctament) per al web

The article presents the requirements of written text to be read on the web, as well as the general recommendations for editing text aimed at facilitating its reading. After introducing the overview of reading of written text, techniques, and strategies, the author turns to factors that condition reading on the web, as well as other specifics. Based on these elements, a series of recommendations is offered for good practices for writing correctly for the web, organised by the three basic components of written text: style or tone, structure, and presentation (orthographic and typographic corrections). Also introduced are tools that can be useful for improving text editing in a corporate environment

Crítical memory of three school debates

As reflection on the education in the Escola Elisava and of the design education in general, the intervention tries to treat the debates that for many years had existed tacitly between the different academic classes, showing the critical situation that it supposes - especially for the fragile design - that one any of these classes influences over the others. From middle of the 90s, the progressive adoption generalized in the higher education of the Anglo-Saxon model ¿with clear predominance of the institutional thing¿ has showed the indicative character of the critique that here is exhibited.

Unedited in vivo detection and quantification of γ-aminobutyric acid in the occipital cortex using short-TE MRS at 3 T.

Short-TE MRS has been proposed recently as a method for the in vivo detection and quantification of γ-aminobutyric acid (GABA) in the human brain at 3 T. In this study, we investigated the accuracy and reproducibility of short-TE MRS measurements of GABA at 3 T using both simulations and experiments. LCModel analysis was performed on a large number of simulated spectra with known metabolite input concentrations. Simulated spectra were generated using a range of spectral linewidths and signal-to-noise ratios to investigate the effect of varying experimental conditions, and analyses were performed using two different baseline models to investigate the effect of an inaccurate baseline model on GABA quantification. The results of these analyses indicated that, under experimental conditions corresponding to those typically observed in the occipital cortex, GABA concentration estimates are reproducible (mean reproducibility error, <20%), even when an incorrect baseline model is used. However, simulations indicate that the accuracy of GABA concentration estimates depends strongly on the experimental conditions (linewidth and signal-to-noise ratio). In addition to simulations, in vivo GABA measurements were performed using both spectral editing and short-TE MRS in the occipital cortex of 14 healthy volunteers. Short-TE MRS measurements of GABA exhibited a significant positive correlation with edited GABA measurements (R = 0.58, p < 0.05), suggesting that short-TE measurements of GABA correspond well with measurements made using spectral editing techniques. Finally, within-session reproducibility was assessed in the same 14 subjects using four consecutive short-TE GABA measurements in the occipital cortex. Across all subjects, the average coefficient of variation of these four GABA measurements was 8.7 ± 4.9%. This study demonstrates that, under some experimental conditions, short-TE MRS can be employed for the reproducible detection of GABA at 3 T, but that the technique should be used with caution, as the results are dependent on the experimental conditions. Copyright © 2013 John Wiley & Sons, Ltd.

Automated scoring of AFLPs using RawGeno v 2.0, a free R CRAN library.

Amplified Fragment Length Polymorphisms (AFLPs) are a cheap and efficient protocol for generating large sets of genetic markers. This technique has become increasingly used during the last decade in various fields of biology, including population genomics, phylogeography, and genome mapping. Here, we present RawGeno, an R library dedicated to the automated scoring of AFLPs (i.e., the coding of electropherogram signals into ready-to-use datasets). Our program includes a complete suite of tools for binning, editing, visualizing, and exporting results obtained from AFLP experiments. RawGeno can either be used with command lines and program analysis routines or through a user-friendly graphical user interface. We describe the whole RawGeno pipeline along with recommendations for (a) setting the analysis of electropherograms in combination with PeakScanner, a program freely distributed by Applied Biosystems; (b) performing quality checks; (c) defining bins and proceeding to scoring; (d) filtering nonoptimal bins; and (e) exporting results in different formats.

Variants : The Journal of the European Society for Textual Scholarship

This volume is the 10th issue of Variants . In keeping with the mission of the European Society for Textual Scholarship, the articles are richly interdisciplinary and transnational. They bring to bear a wide range of topics and disciplines on the field of textual scholarship: historical linguistics, digital scholarly editing, classical philology, Dutch, English, Finnish and Swedish Literature, publishing traditions in Japan, book history, cultural history and folklore. The questions that are explored - what texts are worth editing? what is the nature of the relationship between text, work, document and book? what is a critical digital edition? - all return to fundamental issues that have been at the heart of the editorial discipline for decades. With refreshing insight they assess the increasingly hybrid nature of the theoretical considerations and practical methodologies employed by textual scholars, while reasserting the relevance and need for producing scholarly editions, whether in print or digital, and continuing advanced research in bibliographical codes, textual transmissions, genetic dossiers, the fluidity of texts and other such subjects that connect textual scholarship with broader investigations into our nations' literary culture and written heritage.

Pieces of Iowa’s Past, March 9, 2012

Pieces of Iowa’s Past, published by the Iowa State Capitol Tour Guides weekly during the legislative session, features historical facts about Iowa, the Capitol, and the early workings of state government. All historical publications are reproduced here with the actual spelling, punctuation, and grammar retained. March 7, 2012 THIS WEEK: Orgin of the Historical Department of Iowa BACKGROUND: Charles Aldrich Charles Aldrich served as Iowa Chief Clerk of the House of Representatives for the eighth (1860) and ninth (1862) general assemblies. He volunteered for the Union Army in 1862 and served with the Thirty-second Iowa Infantry for 18 months. Aldrich came to Webster City, Iowa, in 1857. He started the Hamilton Freeman newspaper. He was also involved in the publishing and editing of the Dubuque Times and Marshall County Times. In 1882, at age 53, he represented Hamilton County in the House of Representatives during the 19th General Assembly. He was appointed as the Curator of the State Historical Department at its creation in 1890.

Babel y la Biblioteca, máscaras del mito en la lingüística

Los manuales de historia de la lingüística tratan del mito bíblico de Babel y del nacimiento de la gramática en Alejandría de manos de Dionisio de Tracia. Estos pasajes de la historia, que corresponden a épocas diferentes, remiten a dos problemas fundamentales de la lingüística: por una parte, el origen del lenguaje y la diversidad lingüística; por la otra, la invención de la gramática como instrumento para la edición de textos y para el conocimiento formal de la lengua. Pese a las diferencias, Babel y la biblioteca de Alejandría tienen en común una naturaleza mítica. Sus relatos contienen elementos de la ficción y de la realidad que suelen pasar desapercibidos. El artículo señala estos elementos y establece ciertas afinidades entre Babel y la biblioteca, de suerte que articulados componen un ciclo narrativo. // Abstract.- Babel and the Library, the myth masks in Linguistics. History textbooks of linguistics explain the biblical myth of Babel and the birth of grammar in Alexandria at the hands of Dionysius Thrax. These passages of history, which correspond to different times, refer to two fundamental problems of linguistics: first, the origin of language and linguistic diversity and on the other, the invention of grammar as a tool for text editing and formal knowledge of the language. Despite the differences, Babel and the library of Alexandria share a mythical nature. These stories contain elements of fiction and reality that often go unnoticed. The article points out these elements and establishes affinities between Babel and the library, so that it should be considered as a narrative cycle. Keywords: Babel, Library of Alexandria, myth, story, grammar, institution, Dionysius Thrax.

The Gene Ontology in 2010: extensions and refinements.

The Gene Ontology (GO) Consortium (http://www.geneontology.org) (GOC) continues to develop, maintain and use a set of structured, controlled vocabularies for the annotation of genes, gene products and sequences. The GO ontologies are expanding both in content and in structure. Several new relationship types have been introduced and used, along with existing relationships, to create links between and within the GO domains. These improve the representation of biology, facilitate querying, and allow GO developers to systematically check for and correct inconsistencies within the GO. Gene product annotation using GO continues to increase both in the number of total annotations and in species coverage. GO tools, such as OBO-Edit, an ontology-editing tool, and AmiGO, the GOC ontology browser, have seen major improvements in functionality, speed and ease of use.

Selective resonance suppression 1H-[13C] NMR spectroscopy with asymmetric adiabatic RF pulses.

Despite obvious improvements in spectral resolution at high magnetic field, the detection of 13C labeling by 1H-[13C] NMR spectroscopy remains hampered by spectral overlap, such as in the spectral region of 1H resonances bound to C3 of glutamate (Glu) and glutamine (Gln), and C6 of N-acetylaspartate (NAA). The aim of this study was to develop, implement, and apply a novel 1H-[13C] NMR spectroscopic editing scheme, dubbed "selective Resonance suppression by Adiabatic Carbon Editing and Decoupling single-voxel STimulated Echo Acquisition Mode" (RACED-STEAM). The sequence is based on the application of two asymmetric narrow-transition-band adiabatic RF inversion pulses at the resonance frequency of the 13C coupled to the protons that need to be suppressed during the mixing time (TM) period, alternating the inversion band downfield and upfield from the 13C resonance on odd and even scans, respectively, thus suppressing the detection of 1H resonances bound to 13C within the transition band of the inversion pulse. The results demonstrate the efficient suppression of 1H resonances bound to C3 of Glu and Gln, and C4 of Glu, which allows the 1H resonances bound to C6 of NAA and C4 of Gln to be revealed. The measured time course of the resolved labeling into NAA C6 with the new scheme was consistent with the slow turnover of NAA.

The Catalan migration to New York. A view from La Llumanera de Nova York (1874-1881)

The first issue of La Llumanera de Nova York came out in November 1874 and was not published until 1881. The challenge was certainly complicated: editing a newspaper in Catalan in New York, with the aim of influencing a group of potential readers who were distributed in the United States and, at the same time, become a medium that would bring the ideas of a new era, both from a political and cultural point of view

Sobre las fronteras retóricas del español escrito

El artículo explora la presencia de elementos culturales y retóricos procedentes de la lengua nativa (L1) de un autor en sus escritos en segunda lengua (L2). Se resumen y comentan varias investigaciones de la Retórica Contrastiva, los Nuevos Estudios de Literacidad y la Traductología, que analizan las estrategias usadas por autores y traductores para ocultar o mostrar aspectos de su L1 en sus escritos en L2. También se analizan dos casos de escritura académica (de un latino que escribe en inglés y un quechua que lo hace en español), que aportan ejemplos de extralimitaciones en la corrección y de ocultación de rasgos culturales relevantes. Esos datos y las teorías permiten reflexionar sobre la gestión de la retórica y la cultura en los textos de los plurilingües, tanto desde una óptica de la construcción de la identidad como de la corrección de textos en clase.

'Making Science Understandable'. Divulgació científica i mediació editorial: el cas de The Wisdom of the Body de Walter B. Cannon (1871-1945)

The present paper aims at an historical reconstruction, in the framework of the publishing industry in the years between the two World Wars, of the role played by the publisher William W. Norton in the genesis, published in 1932, and new edition in 1938, of Walter B. Cannon"s book The Wisdom of the Body. With the analysis of this case study, we aimed at contributing to the current criticism of the «dominant view», which tries, in an uncritical manner, that scientific popularization follows an ineluctable, continuous and linear evolution.

SiMR: A simulator for learning computer architecture

This paper presents SiMR, a simulator of the Rudimentary Machine designed to be used in a first course of computer architecture of Software Engineering and Computer Engineering programmes. The Rudimentary Machine contains all the basic elements in a RISC computer, and SiMR allows editing, assembling and executing programmes for this processor. SiMR is used at the Universitat Oberta de Catalunya as one of the most important resources in the Virtual Computing Architecture and Organisation Laboratory, since students work at home with the simulator and reports containing their work are automatically generated to be evaluated by lecturers. The results obtained from a survey show that most of the students consider SiMR as a highly necessary or even an indispensable resource to learn the basic concepts about computer architecture.