Disruption of syntaxin-mediated protein interactions blocks neurotransmitter secretion

The membrane protein syntaxin participates in several protein–protein interactions that have been implicated in neurotransmitter release. To probe the physiological importance of these interactions, we microinjected into the squid giant presynaptic terminal botulinum toxin C1, which cleaves syntaxin, and the H3 domain of syntaxin, which mediates binding to other proteins. Both reagents inhibited synaptic transmission yet did not affect the number or distribution of synaptic vesicles at the presynaptic active zone. Recombinant H3 domain inhibited the interactions between syntaxin and SNAP-25 that underlie the formation of stable SNARE complexes in vitro. These data support the notion that syntaxin-mediated SNARE complexes are necessary for docked synaptic vesicles to fuse.

Morbidity and healthcare utilisation of children in households with one adult: comparative observational study

Objective: To identify and consider differences in morbidity in children in households with one adult presenting to general practitioners compared with children in households with more than one adult.

Brookfield, Massachusetts 15 Minute Digital Raster Graphic

This layer is a digital raster graphic of the historic 15-minute USGS topographic map of the Brookfield, Massachusetts quadrangle. The survey date (ground condition) of this map is 1886-1887, the edition date is April, 1893 and the map was reprinted in 1942. A digital raster graphic (DRG) is a scanned image of a U.S. Geological Survey (USGS) standard series topographic map, including all map collar information. The image inside the map neatline is geo-referenced to the surface of the earth and fit to the Universal Transverse Mercator projection. The horizontal positional accuracy and datum of the DRG matches the accuracy and datum of the source map. The names of quadrangles which border this one appear on the map collar in their respective positions (N,S,E,W) in relation to this map.

Webster, Massachusetts 15 Minute Digital Raster Graphic

This layer is a digital raster graphic of the historic 15-minute USGS topographic map of the Webster, Massachusetts quadrangle. The survey date (ground condition) of the original paper map is 1886-87, the edition date is July, 1892 and this map has a reprint date of 1943. A digital raster graphic (DRG) is a scanned image of a U.S. Geological Survey (USGS) standard series topographic map, including all map collar information. The image inside the map neatline is geo-referenced to the surface of the earth and fit to the Universal Transverse Mercator projection. The horizontal positional accuracy and datum of the DRG matches the accuracy and datum of the source map.

Sinnbilder der Christen /

"Druck und Papier von Friedrich Vieweg in Braunschweig."

Past environment from historical sources /

"PB-231 711."

Reports of decisions made in the Superior Courts of the eastern district of Georgia /

Includes index.

Hawkins's picture of Quebec; with historical recollections.

Compiled, and in part written, by J. C. Fisher. cf. Pref.

The Satires of Persius.

A 2d ed. of the translation of Persius.

Letters to an anxious inquirer : designed to relieve the difficulties of a friend under the serious impressions /

Mode of access: Internet.

Poems,

Mode of access: Internet.

Twelfth night; or, What you will;

Mode of access: Internet.