Effect of treatment with l-amino oxidase fractions, from Bothrops jararaca venom on Ehrlich ascites tumor growth

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

An Isoflavone from Dipteryx alata Vogel is Active against the in Vitro Neuromuscular Paralysis of Bothrops jararacussu Snake Venom and Bothropstoxin I, and Prevents Venom-Induced Myonecrosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases A(2): A Biotechnological Tool to Improve the Production of Antibodies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Temperature and thermal regime effects on the specific dynamic action of Bothrops alternatus (Serpentes, Viperidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

P-I class metalloproteinase from Bothrops moojeni venom is a post-proline cleaving peptidase with kininogenase activity: Insights into substrate selectivity and kinetic behavior

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Rapid purification of serine proteinases from Bothrops alternatus and Bothrops moojeni venoms

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Heterologous expression and biochemical and functional characterization of a recombinant alpha-type myotoxin inhibitor from Bothrops alternatus snake

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Is rainfall seasonality important for reproductive strategies in viviparous Neotropical pit vipers? A case study with Bothrops leucurus from the Brazilian Atlantic Forest

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Acidentes ofídicos por Bothrops jararaca no Estado de São Paulo: influências de variáveis biológicas

No Brasil ocorrem aproximadamente 20.000 acidentes ofídicos anuais, sendo um grande problema de Saúde Pública. As serpentes do gênero Bothrops são responsáveis por aproximadamente 90% dos casos, destacando-se a espécie Bothrops jararaca, responsável por aproximadamente 93% dentre os acidentes botrópicos. O presente trabalho tem como objetivo caracterizar o perfil epidemiológico dos acidentes causados por B. jararaca e avaliar possíveis influências das variáveis biológicas encontradas nos exemplares que causaram acidentes no Estado de São Paulo. Para realização desse estudo foram tomadas como base espécimes de Bothrops jararaca que causaram acidentes nos períodos de 1959 a 2011 preservadas na Coleção Vital Brasil do Instituto Butantan. Essas serpentes (N=1526) foram dissecadas e analisadas. Dados relativos à epidemiologia dos acidentes, período de atividade sazonal, processos reprodutivos e dieta foram coletados. O levantamento e análise dos dados dos acidentes mostraram que 71% dos acidentes são causados por filhotes, enquanto apenas 29% são causados por adultos. Análises preliminares mostraram que fêmeas causaram mais acidentes do que machos independente de serem filhotes ou adultos. O padrão sazonal dos acidentes foi diferente entre os filhotes e adultos de B. jararaca, o que deve estar relacionado às atividades de forrageamento, termorregulação e principalmente aos eventos reprodutivos. O perfil epidemiológico dos acidentados apontou uma maior incidência de acidentes em situações de trabalhos rurais, no caso, a maioria dos acidentados são homens jovens que foram atingidos nas mãos durante o dia

Biogeografia histórica das jararacas do grupo Bothrops alternatus (Serpentes, Viperidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Skeletal muscle degeneration and regeneration after injection of bothropstoxin-II, a phospholipase A2 isolated from the venom of the snake Bothrops jararacussu

A myotoxic phospholipase A2, named bothropstoxin II (BthTX-II), was isolated from the venom of the South American snake Bothrops jararacussu and the pathogenesis of myonecrosis induced by this toxin was studied in mice. BthTX-II induced a rapid increase in plasma creatine kinase levels. Histological and ultrastructural observations demonstrate that this toxin affects muscle fibers by first disrupting the integrity of plasma membrane, as delta lesions were the earliest morphological alteration and since the plasma membrane was interrupted or absent in many portions. In agreement with this hypothesis, BthTX-II released peroxidase entrapped in negatively charged multilamellar liposomes and behaved as an amphiphilic protein in charge shift electrophoresis, an indication that its mechanism of action might be based on the interaction and disorganization of plasma membrane phospholipids. Membrane damage was followed by a complex series of morphological alterations in intracellular structures, most of which are probably related to an increase in cytosolic calcium levels. Myofilaments became hypercontracted into dense clumps which alternated with cellular spaces devoid of myofibrillar material. Later on, myofilaments changed to a hyaline appearance with a more uniform distribution. Mitochondria were drastically affected, showing high amplitude swelling, vesiculation of cristae, formation of flocculent densities, and membrane disruption. By 24 hr, abundant polymorphonuclear leucocytes and macrophages were observed in the interstitial space as well as inside necrotic fibers. Muscle regeneration proceeded normally, as abundant myotubes and regenerating myofibers were observed 7 days after BthTX-II injection. By 28 days regenerating fibers had a diameter similar to that of adult muscle fibers, although they presented two distinctive features: central location of nuclei and some fiber splitting. This good regenerative response may be explained by the observation that BthTX-II does not affect blood vessels, nerves, or basal laminae. © 1991.

Preliminary X-ray crystallographic studies of a Lys49-phospholipase A(2) homologue from Bothrops pirajai venom complexed with p-bromophenacyl bromide and alpha-tocopherol inhibitors

PrTX-I, a non-catalytic and myotoxic Lys49-PLA(2) from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), alpha-tocopherol and alpha-tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 angstrom resolution. All complexes crystals are isomorphous and belong to the space group P2(1) whereas native PrTX-I crystals belong to the P3(1)21.