The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation


Autoria(s): Nishijima, Catarine Massucato; Delella, Flavia Karina; Rodrigues, Clenilson Martins; Rinaldo, Daniel; Anjos Lopes-Ferreira, Monica Valdyrce dos; Machado da Rocha, Lucia Regina; Vilegas, Wagner; Felisbino, Sergio Luis; Hiruma-Lima, Clelia Akiko
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

21/10/2015

21/10/2015

01/06/2015

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Inflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

Formato

12454-12466

Identificador

http://www.mdpi.com/1422-0067/16/6/12454

International Journal Of Molecular Sciences, v. 16, n. 6, p. 12454-12466, 2015.

1422-0067

http://hdl.handle.net/11449/129557

http://dx.doi.org/10.3390/ijms160612454

WOS:000357492800038

Idioma(s)

eng

Publicador

Mdpi Ag

Relação

International Journal Of Molecular Sciences

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article