Detection of sandal spike phytoplasma using immunological and molecular techniques

Spike disease in sandal is generally diagnosed by the manifestation of external symptoms. Attempts have been made to detect the diseased plants by determining the length/breadth ratio of leaves (lyengar, 1961) and histochemical tests using Mann's stain (Parthasarathi et al., 1966), Dienes' stain (Ananthapadmanabha et a/., 1973) aniline blue and Hoechst 33258 (Ghosh et a/., 1985, Rangaswamy, 1995). But most of these techniques are insensitive, indirect detection methods leading to misinterpretation of results. Moreover, to identify disease resistant sandal trees, highly sensitive techniques are needed to detect the presence of the pathogen. In sandal forests, several host plants of sandal like Zizyphus oenop/ea (Fig. 1.3) also exhibit the yellows type disease symptoms. Immunological and molecular assays have to be developed to confirm the presence of sandal spike phytoplasma in such hosts. The major objectives of the present work includes:In situ detection of sandal spike phytoplasma by epifluorescence microscopy and scanning electron microscopy.,Purification of sandal spike phytoplasma and production of polyclonal antibodies.,Amino acid and total protein estimation of sandal spike phytoplasma.,Immunological detection of sandal spike phytoplasma., Molecular detection of sandal spike phytoplasma.,Screening for phytoplasma in host plants of spike disease affected sandal using immunological and molecular techniques.

“Improved Feature Extraction and Classification Techniques for Multispectral Brain Magnetic Resonance Images”

Magnetic Resonance Imaging (MRI) is a multi sequence medical imaging technique in which stacks of images are acquired with different tissue contrasts. Simultaneous observation and quantitative analysis of normal brain tissues and small abnormalities from these large numbers of different sequences is a great challenge in clinical applications. Multispectral MRI analysis can simplify the job considerably by combining unlimited number of available co-registered sequences in a single suite. However, poor performance of the multispectral system with conventional image classification and segmentation methods makes it inappropriate for clinical analysis. Recent works in multispectral brain MRI analysis attempted to resolve this issue by improved feature extraction approaches, such as transform based methods, fuzzy approaches, algebraic techniques and so forth. Transform based feature extraction methods like Independent Component Analysis (ICA) and its extensions have been effectively used in recent studies to improve the performance of multispectral brain MRI analysis. However, these global transforms were found to be inefficient and inconsistent in identifying less frequently occurred features like small lesions, from large amount of MR data. The present thesis focuses on the improvement in ICA based feature extraction techniques to enhance the performance of multispectral brain MRI analysis. Methods using spectral clustering and wavelet transforms are proposed to resolve the inefficiency of ICA in identifying small abnormalities, and problems due to ICA over-completeness. Effectiveness of the new methods in brain tissue classification and segmentation is confirmed by a detailed quantitative and qualitative analysis with synthetic and clinical, normal and abnormal, data. In comparison to conventional classification techniques, proposed algorithms provide better performance in classification of normal brain tissues and significant small abnormalities.

Memory, revision and exam techniques

University of Southampton, Dyslexia Services have developed a range of academic study skills resources available to download. This resource supports revision and techniques for use in examinations.

More women in informatics research and education

Speaker: Lynda Hardman Organiser: Time: 04/02/2015 12:30-13:30 Location: B32/3077 Abstract The challenges of addressing gender inequalities in science, technology, engineering, mathematics and medicine is widely acknowledged. We currently hold a bronze award and ECS is one of many academic units in the University which has gained Athena Swan Charter status. In this seminar, Professor Lynda Hardman, Chair of the Informatics Europe working group "Women in Informatics Research and Education” will be explaining the causes of issued underlying gender inequality and constructive routes to addressing this important agenda. In undertaking to commit to an action plan which is a prerequisite of gaining charter status, the University or academic department agreed to accept and incorporate the Athena Swan six principles listed below: * To address gender inequalities requires commitment and action from everyone, at all levels of the organisation * To tackle the unequal representation of women in science requires changing cultures and attitudes across the organisation * The absence of diversity at management and policy-making levels has broad implications which the organisation will examine * The high loss rate of women in science is an urgent concern which the organisation will address * The system of short-term contracts has particularly negative consequences for the retention and progression of women in science, which the organisation recognises * There are both personal and structural obstacles to women making the transition from PhD into a sustainable academic career in science, which require the active consideration of the organisation. This seminar is designed to provide an opportunity to explore these issues NOTE: Lynda will be basing here talk on some of the work she directed as chair of the "Women in Informatics Research and Education” working group. The purpose of the working group is to actively participate and promote actions that contribute to improve gender balance in Information and Communication Sciences and Technologies. The first concrete result of the working group's activities was the publication of the booklet "More Women in Informatics Research and Education" in 2013. The booklet is a compact source of clear and simple best practices to deans and heads of departments that aim to increase the participation of women as both students and employees in their institutions. Many tips included were also inspired by colleagues already in leading positions who have already implemented actions in their institutions to attract more women and ensure their continued participation in the organization at commensurate ratios with their male colleagues. The booklet is endorsed by the European Commission and features a foreword by Neelie Kroes, Vice-President of the European Commission, responsible for the Digital Agenda.

BTEC First engineering. Mandatory and selected optional units for BTEC firsts in engineering.

Este recurso ayuda al alumno a superar el curso BTEC First, con el que obtiene la titulación en ingeniería, uno de los sectores profesionales de este programa de estudios. Se divide en ocho unidades, algunas de las cuales son unidades obligatorias para la consecución del título y otras unidades son opcionales. Se dedica un capítulo a cada unidad y cada uno de estos capítulos contiene texto, ilustraciones, ejemplos, prueba de preguntas de conocimientos, actividades y un conjunto de preguntas de repaso.

Mathematics, computers in Mathematics, and gender : public perceptions in context. 'Matemáticas, ordenadores en Matemáticas y género : percepciones públicas en contexto'.

Resumen basado en el de la publicación

Assessment of virtual design and manufacturing techniques for fibre reinforced composite materials

Virtual tools are commonly used nowadays to optimize product design and manufacturing process of fibre reinforced composite materials. The present work focuses on two areas of interest to forecast the part performance and the production process particularities. The first part proposes a multi-physical optimization tool to support the concept stage of a composite part. The strategy is based on the strategic handling of information and, through a single control parameter, is able to evaluate the effects of design variations throughout all these steps in parallel. The second part targets the resin infusion process and the impact of thermal effects. The numerical and experimental approach allowed the identificationof improvement opportunities regarding the implementation of algorithms in commercially available simulation software.

Understanding safety and production risks in rail engineering planning and protection

Much of the published human factors work on risk is to do with safety and within this is concerned with prediction and analysis of human error and with human reliability assessment. Less has been published on human factors contributions to understanding and managing project, business, engineering and other forms of risk and still less jointly assessing risk to do with broad issues of ‘safety’ and broad issues of ‘production’ or ‘performance’. This paper contains a general commentary on human factors and assessment of risk of various kinds, in the context of the aims of ergonomics and concerns about being too risk averse. The paper then describes a specific project, in rail engineering, where the notion of a human factors case has been employed to analyse engineering functions and related human factors issues. A human factors issues register for potential system disturbances has been developed, prior to a human factors risk assessment, which jointly covers safety and production (engineering delivery) concerns. The paper concludes with a commentary on the potential relevance of a resilience engineering perspective to understanding rail engineering systems risk. Design, planning and management of complex systems will increasingly have to address the issue of making trade-offs between safety and production, and ergonomics should be central to this. The paper addresses the relevant issues and does so in an under-published domain – rail systems engineering work.

A importância da matemática e da modelação matemática no mestrado integrado em ciências farmacêuticas

A Matemática e as Ciências Farmacêuticas encontram-se relacionadas desde há muito, no entanto, foi a partir do séc. XVII, período de notável agitação cultural e científico que os métodos experimentais foram sustentados com cálculos matemáticos. Esta ciência e as técnicas de modelagem matemática tornaram-se numa ferramenta amplamente utilizada, de tal modo, que nos dias de hoje são consideradas como fundamentais na generalidade das profissões e em especial nas Ciências Farmacêuticas. Contudo, para muitos ainda não é vista como fundamental e essencial para a formação de futuros farmacêuticos. Deste modo, pretende-se demonstrar como a Matemática e as técnicas de modelagem se tornaram ao longo dos anos nesta poderosa ferramenta. Quer pelos instrumentos, quer pelas competências que nos proporcionam. Pretende-se também, com recurso aos conteúdos programáticos desta unidade curricular, avaliar se os conhecimentos, sistemas de avaliação e distribuição da carga horária são efetuados de forma homogénea pelas diferentes instituições portuguesas, públicas ou privadas que lecionam o Mestrado Integrado em Ciências Farmacêuticas. Verificou-se que a Matemática é uma ciência plena de capacidades e recursos e que estabelece uma relação interdisciplinar com as Ciências Farmacêuticas. Quer pela componente utilitária, quer pela componente formativa que proporciona. A análise dos conteúdos programáticos demonstra que apesar de serem transversais, as Universidades que não lecionam Sistemas de Equações Lineares e Equações diferenciais deveriam faze-lo e também realizarem um melhor controlo da carga horária por temática.

Patient perception of disability following acoustic (vestibular) schwannoma removal via gamma knife and microsurgical techniques

This study evaluates patient's short and long-term balance function after microsurgical tumor removal and gamma knife radiosurgery using an unvalidated qualitative questionnaire and the Dizziness Handicap Inventory.