Fit genotypes and escape variants of subgroup III Neisseria meningitidis during three pandemics of epidemic meningitis

The genetic variability at six polymorphic loci was examined within a global collection of 502 isolates of subgroup III, serogroup A Neisseria meningitidis. Nine “genoclouds” were identified, consisting of genotypes that were isolated repeatedly plus 48 descendent genotypes that were isolated rarely. These genoclouds have caused three pandemic waves of disease since the mid-1960s, the most recent of which was imported from East Asia to Europe and Africa in the mid-1990s. Many of the genotypes are escape variants, resulting from positive selection that we attribute to herd immunity. Despite positive selection, most escape variants are less fit than their parents and are lost because of competition and bottlenecks during spread from country to country. Competition between fit genotypes results in dramatic changes in population composition over short time periods.

Nuclear particles containing RNA polymerase III complexes associated with the junctional plaque protein plakophilin 2

Plakophilin 2, a member of the arm-repeat protein family, is a dual location protein that occurs both in the cytoplasmic plaques of desmosomes as an architectural component and in an extractable form in the nucleoplasm. Here we report the existence of two nuclear particles containing plakophilin 2 and the largest subunit of RNA polymerase (pol) III (RPC155), both of which colocalize and are coimmunoselected with other pol III subunits and with the transcription factor TFIIIB. We also show that plakophilin 2 is present in the pol III holoenzyme, but not the core complex, and that it binds specifically to RPC155 in vitro. We propose the existence of diverse nuclear particles in which proteins known as plaque proteins of intercellular junctions are complexed with specific nuclear proteins.

The Danish Flexicurity Model - a Lesson for the US? ACES Working Paper No. 2, May 2007

Within recent years, increasing international competition has caused an increase in job transitions worldwide. Many countries find it difficult to manage these transitions in a way that ensures a match between labour and demand. One of the countries that seem to manage the transitions in a successful way is Denmark, where unemployment has been dropping dramatically over the last decade without a drop in job quality. This success is ascribed the so-called Danish flexicurity model, where an easy access to hiring and firing employees (flexibility) is combined with extensive active and passive labour market policies (security). The Danish results have gained interest not only among other European countries, where unemployment rates remain high, but also in the US, where job loss is often related to lower job quality. It has, however, been subject to much debate both in Europe and in the US, whether or not countries with distinctively different political-economic settings can learn from one another. Some have argued that cultural differences impose barriers to successful policy transfer, whereas others see it as a perfectly rational calculus to introduce 'best practices' from elsewhere. This paper presents a third strategy. Recent literature on policy transfer suggests that successful cross national policy transfer is possible, even across the Atlantic, but that one must be cautious in choosing the form, content and level of the learning process. By analysing and comparing the labour market policies and their settings in Denmark and the US in detail, this paper addresses the question, what and how the US can learn from the Danish model. Where the US and Denmark share a high degree of flexibility, they differ significantly on the level of security. This also means that the Danish budget for active and passive labour market policies is significantly higher than the American, and it seems unlikely that political support for the introduction of Danish levels of security in the US can be established. However, the paper concludes that there is a learning potential between the US and Demnark in the different local level efficiency of the money already spent. A major reason for the Danish success has been the introduction of tailor made initiatives to the single displaced worker and a stronger coordination between local level actors. Both of which are issues, where a lack of efficiency in the implementation of American active labour market policies has been reported.

Extranjeridad : Lengua y traducción en la obra de Manuel Puig

En este artículo, nos proponemos examinar cómo el translingüismo de Puig se conjuga con apuestas muy personales de su proyecto artístico, ya que elegir una lengua en lugar de o en contra de otra raramente se debe al azar. Para empezar, es sintomático de la forma de Puig de situarse extraterritorial, como un exiliado permanente que veía con ojos extranjeros todas las sociedades donde había vivido. Pero va más allá, ya que encaja en el polimorfismo identitario que caracterizaba al autor argentino, en su empeño por ser múltiple y su voluntad de ensanchar los límites de la literatura

Extranjeridad : Lengua y traducción en la obra de Manuel Puig

En este artículo, nos proponemos examinar cómo el translingüismo de Puig se conjuga con apuestas muy personales de su proyecto artístico, ya que elegir una lengua en lugar de o en contra de otra raramente se debe al azar. Para empezar, es sintomático de la forma de Puig de situarse extraterritorial, como un exiliado permanente que veía con ojos extranjeros todas las sociedades donde había vivido. Pero va más allá, ya que encaja en el polimorfismo identitario que caracterizaba al autor argentino, en su empeño por ser múltiple y su voluntad de ensanchar los límites de la literatura

Distribution of halacarid species in sediment surface samples of Josephine Bank and Great Meteor Bank, Northeast Atlantic (Table 4)

In 22 samples, 6 from Josephine Bank and 16 from the Great Meteor Bank, 14 halacarid species were found and described. Halacarus spiniger n. sp., Copidognathus magniporus n. sp., Arhodeoporus lineatus n. sp., A. brevocularis n. sp., Coloboceras karamani n. sp., Scaptognathus minutus n. sp., and Atelopsalis newelli were hithero unknown. Acaromantis squilla Trouessart & Neumann and Atelopsalis tricuspis Trouessart were redescribed. Four larvae, probably belonging to Copidognathus longips Bartsch, C. tricorneata (Lohmann), Lohmannella falcata (Hodge), and Atelopsalis newelli n. sp. were described, two Scaptognathus larvae could not be identifird. To date only three species, Copidognathus tricorneata, Lohmannella falcata, and Scaptognathus minutus, have been found on both seamounts.

U-M Photo Services photo lab, ca 1960

[L-R? Audrey "Audie" Hendon -customer rep, Ozalid operator; Robert Kalmbach- dark room printer; Lajos "Louis" Martonyi - photographer; Carmen Krasteff - Ozalid operator; Fred Anderegg - supervisor photographer; Karloyi "Karl" Kutasi - photographer; Lorene Fitzgerald - secretary; Willie Dobos - photographer; Wilhelmine Hoesl - lab assistant, Ilse Wiener - photostat operator, Karl Kalmbach - darkroom printer]

New images of man. With statements by the artists.

Errata slip inserted.

Extranjeridad : Lengua y traducción en la obra de Manuel Puig

En este artículo, nos proponemos examinar cómo el translingüismo de Puig se conjuga con apuestas muy personales de su proyecto artístico, ya que elegir una lengua en lugar de o en contra de otra raramente se debe al azar. Para empezar, es sintomático de la forma de Puig de situarse extraterritorial, como un exiliado permanente que veía con ojos extranjeros todas las sociedades donde había vivido. Pero va más allá, ya que encaja en el polimorfismo identitario que caracterizaba al autor argentino, en su empeño por ser múltiple y su voluntad de ensanchar los límites de la literatura

Regulation of MAPK activation, AP-1 transcription factor expression and keratinocyte differentiation in wounded fetal skin

Fetal epithelium retains the ability to re-epithelialize a wound in organotypic culture in a manner not dependent on the presence of underlying dermal substrata. This capacity is lost late in the third trimester of gestation or after embryonic day 17 (E-17) in the rat such that embryonic day 19 (E-19) wounds do not re-epithelialize. Moreover, wounds created in E-17 fetuses in utero heal in a regenerative, scar-free fashion. To investigate the molecular events regulating re-epithelialization in fetal skin, the wound-induced expression profile and tissue localization of activator protein 1 (AP-1) transcription factors c-Fos and c-Jun was characterised in E-17 and E-19 skin using organotypic fetal cultures. The involvement of mitogen-activated protein kinase (MAPK) signaling in mediating wound-induced transcription factor expression and wound re-epithelialization was assessed, with the effect of wounding on the expression of keratinocyte differentiation markers determined. Our results show that expression of AP-1 transcription factors was induced immediately by wounding and localized predominantly to the epidermis in E-17 and E-19 skin. c-fos and c-jun induction was transient in E-17 skin with MAPK-dependent c-fos expression necessary for the re-epithelialization of an excisional wound in organotypic culture. In E-19 skin, AP-11 expression persisted beyond 12 h post-wounding, and marked upregulation of the keratinocyte differentiation markers keratin 10 and loricrin was observed. No such changes in the expression of keratin 10 or loricrin occurred in E-17 skin. These findings indicate that re-epithelialization in fetal skin is regulated by wound-induced AP-1 transcription factor expression via MAPK and the differentiation status of keratinocytes.

Diagnosing autism spectrum disorder:who will get a DSM-5 diagnosis?

Background Introduction of proposed criteria for DSM-5 Autism Spectrum Disorder (ASD) has raised concerns that some individuals currently meeting diagnostic criteria for Pervasive Developmental Disorder (PDD; DSM-IV-TR/ICD-10) will not qualify for a diagnosis under the proposed changes. To date, reports of sensitivity and specificity of the new criteria have been inconsistent across studies. No study has yet considered how changes at the 'sub domain' level might affect overall sensitivity and specificity, and few have included individuals of different ages and ability levels. Methods A set of DSM-5 ASD algorithms were developed using items from the Diagnostic Interview for Social and Communication Disorders (DISCO). The number of items required for each DSM-5 subdomain was defined either according to criteria specified by DSM-5 (Initial Algorithm), a statistical approach (Youden J Algorithm), or to minimise the number of false positives while maximising sensitivity (Modified Algorithm). The algorithms were designed, tested and compared in two independent samples (Sample 1, N = 82; Sample 2, N = 115), while sensitivity was assessed across age and ability levels in an additional dataset of individuals with an ICD-10 PDD diagnosis (Sample 3, N = 190). Results Sensitivity was highest in the Initial Algorithm, which had the poorest specificity. Although Youden J had excellent specificity, sensitivity was significantly lower than in the Modified Algorithm, which had both good sensitivity and specificity. Relaxing the domain A rules improved sensitivity of the Youden J Algorithm, but it remained less sensitive than the Modified Algorithm. Moreover, this was the only algorithm with variable sensitivity across age. All versions of the algorithm performed well across ability level. Conclusions This study demonstrates that good levels of both sensitivity and specificity can be achieved for a diagnostic algorithm adhering to the DSM-5 criteria that is suitable across age and ability level. © 2013 The Authors. Journal of Child Psychology and Psychiatry © 2013 Association for Child and Adolescent Mental Health.