Polyphasic approach including MALDI-TOF MS/MS analysis for identification and characterisation of Fusarium verticillioides in Brazilian corn kernels

Fusarium verticillioides is considered one of the most important global sources of fumonisin contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. verticillioides species isolated from corn kernels in 3 different Regions of the Brazilian State of Pernambuco. A polyphasic approach including classical taxonomy, molecular biology, MALDI-TOF MS and MALDI-TOF MS/MS for the identification and characterisation of the F. verticillioides strains was used. Sixty F. verticillioides strains were isolated and successfully identified by classical morphology, proteomic profiles of MALDI-TOF MS, and by molecular biology using the species-specific primers VERT-1 and VERT-2. FUM1 gene was further detected for all the 60 F. verticillioides by using the primers VERTF-1 and VERTF-2 and through the amplification profiles of the ISSR regions using the primers (GTG)5 and (GACA)4. Results obtained from molecular analysis shown a low genetic variability among these isolates from the different geographical regions. All of the 60 F. verticillioides isolates assessed by MALDI-TOF MS/MS presented ion peaks with the molecular mass of the fumonisin B1 (721.83 g/mol) and B2 (705.83 g/mol)

A Prova por reconhecimento de pessoas produzida ex novo em sede de audi��ncia de julgamento: fiabilidade e impacto na descoberta da verdade material

Disserta����o de mestrado em Direito Judici��rio (Direitos Processuais e Organiza����o Judici��ria)

Comparative study of smokers, ex-smokers, and nonsmokers who have experienced myocardial infarction

OBJECTIVE: To assess the impact of smoking on in-hospital morbidity and mortality in patients who have experienced acute myocardial infarction and to assess the association between smoking and other cardiovascular risk factors and clinical data. METHODS: A prospective cohort study analyzed 121 patients, including 54 smokers, 35 ex-smokers, and 32 nonsmokers. RESULTS: Using the chi-square test (P<0.05), an association between smoking and the risk factors sex, age, and diabetes was documented. Among the morbidity and mortality variables, only acute pulmonary edema showed a statistically significant difference (OR=9.5; 95% CI), which was greater in the ex-smoker group than in the nonsmoker group. CONCLUSION: An association between smoking and some cardiovascular risk factors was observed, but no statistical difference in morbidity and mortality was observed in the groups studied, except for the variable acute pulmonary edema.

Museum botanicum Lugduno-Batavum, sive, Stirpium exoticarum novarum vel minus cognitarum ex vivis aut siccis brevis expositio et descriptio

t.2 (1852-1856)

Museum botanicum Lugduno-Batavum, sive, Stirpium exoticarum novarum vel minus cognitarum ex vivis aut siccis brevis expositio et descriptio

t.1 (1849-1851) [Incomplete]

Flora germanica excursoria ex affinitate regni vegetabilis naturali disposita, sive principia synopseos plantarum in Germania terrisque in Europa media adjacentibus sponte nascentium cultarumque frequentius /

v.1 (1830-1832)

Flora germanica excursoria ex affinitate regni vegetabilis naturali disposita, sive principia synopseos plantarum in Germania terrisque in Europa media adjacentibus sponte nascentium cultarumque frequentius /

v.2 (1830-1832)

Icones selectae plantarum quas in systemate universali :ex herbariis parisiensibus, praesertim ex Lessertiano /descripsit Aug. Pyr. de Candolle, ex archetypis speciminibus a P.J.F. Turpin delineatae et editae a Benj. De Lessert ...

vol. 1

Icones selectae plantarum quas in systemate universali :ex herbariis parisiensibus, praesertim ex Lessertiano /descripsit Aug. Pyr. de Candolle, ex archetypis speciminibus a P.J.F. Turpin delineatae et editae a Benj. De Lessert ...

vol. 2

Icones selectae plantarum quas in systemate universali :ex herbariis parisiensibus, praesertim ex Lessertiano /descripsit Aug. Pyr. de Candolle, ex archetypis speciminibus a P.J.F. Turpin delineatae et editae a Benj. De Lessert ...

vol. 3

Icones selectae plantarum quas in systemate universali :ex herbariis parisiensibus, praesertim ex Lessertiano /descripsit Aug. Pyr. de Candolle, ex archetypis speciminibus a P.J.F. Turpin delineatae et editae a Benj. De Lessert ...

vol. 4

Icones selectae plantarum quas in systemate universali :ex herbariis parisiensibus, praesertim ex Lessertiano /descripsit Aug. Pyr. de Candolle, ex archetypis speciminibus a P.J.F. Turpin delineatae et editae a Benj. De Lessert ...

vol. 5

Mantissa in volumen primum [-tertium] :Systematis vegetabilium caroli a Linne�� : ex editione Joan. Jac. Roemer et Jos. Aug. Schultes /curante I.A. Schultes.

v.1, c.2

Mantissa in volumen primum [-tertium] :Systematis vegetabilium caroli a Linne�� : ex editione Joan. Jac. Roemer et Jos. Aug. Schultes /curante I.A. Schultes.

v.2, c.2