1942 UM Track Team

Top Row: Roosevelt Stiger, Joe Lahey, Livius Stroia, Jim Sears, Hank Schmidt

3rd Row: ass't coach Chester Stackhouse, John McKean, John Ingersoll, James Byerly, Gene Hirsch, Willie Gies, John Roxborough, Charles Donahey

2nd Row: Ernie Leonardi, Charles Pinney, Buell Morley, George Ostroot, Will Ackerman, Bob Segula, st. mngr. Chuck Boynton.

Front Row: George Pettersen, Frank McCarthy, Al Thomas, captain Alfred Piel, Coach Ken Doherty, Dave Matthews, John Kautz, Bob Ufer

University of Michigan Football Team, 1934, full squad posed on practice field

Back Row: Cedric Sweet, Franklin Lett, Steve Remias, Carl Carr, Russ Oliver, captain Thomas Austin, Robert Johnson, Willard Hildebrandt, Edward Stone, Michael Savage, Thomas Oyler, Harry Wright, Joe Fisher, Jack Liffiton, John Viergever, Herman Everhardus, Willis Ward, Matt Patanelli, Robert Graper

Middle Row: John Mumford, James Kidston, William Borgmann, Chester Beard, Howard Triplehorn, Vincent Pope, Ernest Pederson, Joseph Ellis, Robert Amrine, Russell Fuog, Stanton Schuman, Vincent Aug, Winfred Nelson, John Regeczi

Front Row: John Connolly, Jesse Garber, David Barnett, John Rieck, Frank Bissell, Eli Soodik, William Renner, Richard James, Ferris Jennings, Harry Pillinger, Harry Lutomski, George Bolas, Charles Brandman, Gerald Ford

University of Michigan Football Team, 1934, full squad posed on practice field

Back Row: Cedric Sweet, Franklin Lett, Steve Remias, Carl Carr, Russ Oliver, captain Thomas Austin, Robert Johnson, Willard Hildebrandt, Edward Stone, Michael Savage, Thomas Oyler, Harry Wright, Joe Fisher, Jack Liffiton, John Viergever, Herman Everhardus, Willis Ward, Matt Patanelli, Robert Graper

Middle Row: John Mumford, James Kidston, William Borgmann, Chester Beard, Howard Triplehorn, Vincent Pope, Ernest Pederson, Joseph Ellis, Robert Amrine, Russell Fuog, Stanton Schuman, Vincent Aug, Winfred Nelson, John Regeczi

Front Row: John Connolly, Jesse Garber, David Barnett, John Rieck, Frank Bissell, Eli Soodik, William Renner, Richard James, Ferris Jennings, Harry Pillinger, Harry Lutomski, George Bolas, Charles Brandman, Gerald Ford

The history and antiqvities of Filey, in the covnty of York.

Engr. t.-p.

Strictures on Montgomery on the cotton manufactures of Great Britain and America. Also, a practical comparison of the cost of steam and water power in American.

Mode of access: Internet.

Walker's pronouncing dictionary of the English language : abridged for the use of schools : containing a compendium of the principles of English pronunciation with the proper names ...

Mode of access: Internet.

The modern British Plutarch; or, Lives of men distinguished in the recent history of England for their talents, virtues, or achievements.

Richard Arkwright.--Edmund Burke.--Robert Burns.--Lord Byron.--George Canning.--Earl of Chatham.--Dr. Adam Clarke.--Lord Clive.--Captain Cook.--William Cowper.--Rev. George Crabbe.--Sir Humphrey Davy.--Lord Eldon.--Lord Erskine.--Charles James Fox.--Benjamin Franklin.--Oliver Goldsmith.--Henry Grattan.--Earl Grey.--Warren Hastings.--Bishop Heber.--John Howard.--Dr. Jenner.--Sir William Jones.--Sir James Mackintosh.--Rev. Henry Martyn, B.D.--Sir John Moore, K.B.--Lord Nelson.--William Pitt.--Sir Samuel Romilly.--Sir Walter Scott.--Richard Brinsley Sheridan.--John Smeaton.--James Watt.--Marquis of Wellesley.--William Wilberforce.--Sir David Wilkie.--Duke of Wellington.

An introduction to the elements of algebra, designed for the use of those who are acquainted only with the first principles of arithmetic.

"The selections ... are from the first English edition."--Advertisement.

Wildlife and America : contributions to an understanding of American wildlife and its conservation /

Cosponsored by U.S. Fish and Wildlife Service, Forest Service, and the National Oceanic and Atmospheric Administration.

Transient production of recombinant proteins by chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents

We have developed a simple and robust transient expression system utilizing the 25 kDa branched cationic polymer polyethylenimine (PEI) as a vehicle to deliver plasmid DNA into suspension-adapted Chinese hamster ovary cells synchronized in G2/M phase of the cell cycle by anti-mitotic microtubule disrupting agents. The PEI-mediated transfection process was optimized with respect to PEI nitrogen to DNA phosphate molar ratio and the plasmid DNA mass to cell ratio using a reporter construct encoding firefly luciferase. Optimal production of luciferase was observed at a PEI N to DNA P ratio of 10:1 and 5 mug DNA 10(6) cells(-1). To manipulate transgene expression at mitosis, we arrested cells in G2/M phase of the cell cycle using the microtubule depolymerizing agent nocodazole. Using secreted human alkaline phosphatase (SEAP) and enhanced green fluorescent protein (eGFP) as reporters we showed that continued inclusion of nocodazole in cell culture medium significantly increased both transfection efficiency and reporter protein production. In the presence of nocodazole, greater than 90% of cells were eGFP positive 24 h post-transfection and qSEAP was increased almost fivefold, doubling total SEAP production. Under optimal conditions for PEI-mediated transfection, transient production of a recombinant chimeric IgG(4) encoded on a single vector was enhanced twofold by nocodazole, a final yield of approximately 5 mug mL(-1) achieved at an initial viable cell density of 1 x 10(6) cells mL(-1). The glycosylation of the recombinant antibody at Asn(297) was not significantly affected by nocodazole during transient production by this method. (C) 2004 Wiley Periodicals, Inc.

On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by CHO cells

Monoclonal antibodies (Mab) are heterotetramers consisting of an equimolar ratio of heavy chain (HC) and light chain (LC) polypeptides. Accordingly, most recombinant Mab expression systems utilize an equimolar ratio of heavy chain (he) to light chain (lc) genes encoded on either one or two plasmids. However, there is no evidence to suggest that this gene ratio is optimal for stable or transient production of recombinant Mab. In this study we have determined the optimal ratio of hc:lc genes for production of a recombinant IgG(4) Mab, cB72.3, by Chinese hamster ovary (CHO) cells using both empirical and mathematical modeling approaches. Polyethyleneimine-mediated transient expression of cB72.3 at varying ratios of hc:lc genes encoded on separate plasmids yielded an optimal Mab titer at a hc:lc gene ratio of 3:2; a conclusion confirmed by separate mathematical modeling of the Mab folding and assembly process using transient expression data. On the basis of this information, we hypothesized that utilization of he genes at low hc:lc gene ratios is more efficient. To confirm this, cB72.3 Mab was transiently produced by CHO cells at constant he and varying lc gene dose. Under these conditions, Mab yield was increased with a concomitant increase in lc gene dose. To determine if the above findings also apply to stably transfected CHO cells producing recombinant Mab, we compared the intra- and extracellular ratios of HC and LC polypeptides for three GS-CHO cells lines transfected with a 1:1 ratio of hc:lc genes and selected for stable expression of the same recombinant Mab, cB72.3. Intra- and extracellular HC:LC polypeptide ratios ranged from 1:2 to 1:5, less than that observed on transient expression of the same Mab in parental CHO cells using the same vector. In conclusion, our data suggest that the optimal ratio of hc:lc genes used for transient and stable expression of Mab differ. In the case of the latter, we infer that optimal Mab production by stably transfected cells represents a compromise between HC abundance limiting productivity and the requirement for excess LC to render Mab folding and assembly more efficient.

Participation in the RACS sentinel node biopsy versus axillary clearance trial

Background: The Royal Australasian College of Surgeons (RACS) SNAC trial is a randomized controlled trial of sentinel node biopsy (SNB) versus axillary clearance (AC). It opened in May 2001 and is recruiting rapidly with good acceptance by consumers. Methods: A study of eligibility and treatment choices was conducted between November 2001 and September 2002 for women presenting with early breast cancer to 10 centres participating in the trial. Results: More than half of the 622 women (54%) were ineligible for trial entry because they had large (> 3 cm) or multicentric cancers. Participation was offered to 92% of eligible women and was taken up by 63%. The commonest reason for not participating was the desire to choose treatment rather than have it randomly allocated. Despite this there is a great acceptance of clinical trials because very few women (4% of those eligible) gave 'lack of interest in clinical trials' as the reason for non-participation. Few women who declined trial participation chose to have SNB alone (4.5% of those eligible). Conclusion: Sentinel node biopsy may become the standard of care for managing small breast cancers, but a significant number of patients will still require or choose axillary dissection. Results from large randomized trials are needed to determine the relative benefits and harms of SNB compared with AC. Surgeons must carefully discuss options for management with their patients.

Adherence to antiepileptic medicines in children:a multiple-methods assessment involving dried blood spot sampling

Purpose - To evaluate adherence to prescribed antiepileptic drugs (AEDs) in children with epilepsy using a combination of adherence-assessment methods. Methods - A total of 100 children with epilepsy (≤17 years old) were recruited. Medication adherence was determined via parental and child self-reporting (≥9 years old), medication refill data from general practitioner (GP) prescribing records, and via AED concentrations in dried blood spot (DBS) samples obtained from children at the clinic and via self- or parental-led sampling in children's own homes. The latter were assessed using population pharmacokinetic modeling. Patients were deemed nonadherent if any of these measures were indicative of nonadherence with the prescribed treatment. In addition, beliefs about medicines, parental confidence in seizure management, and the presence of depressed mood in parents were evaluated to examine their association with nonadherence in the participating children. Key Findings - The overall rate of nonadherence in children with epilepsy was 33%. Logistic regression analysis indicated that children with generalized epilepsy (vs. focal epilepsy) were more likely (odds ratio [OR] 4.7, 95% confidence interval [CI] 1.37–15.81) to be classified as nonadherent as were children whose parents have depressed mood (OR 3.6, 95% CI 1.16–11.41). Significance - This is the first study to apply the novel methodology of determining adherence via AED concentrations in clinic and home DBS samples. The present findings show that the latter, with further development, could be a useful approach to adherence assessment when combined with other measures including parent and child self-reporting. Seizure type and parental depressed mood were strongly predictive of nonadherence.