Phylogenetic analyses and toxigenic profiles of Fusarium equiseti and Fusarium acuminatum isolated from cereals from Southern Europe

Fusarium equiseti and Fusarium acuminatum are toxigenic species that contaminate cereal crops from diverse climatic regions. They are common in Spanish cereals. The information available on their phylogenetics and toxigenic profiles is, however, insufficient to assist risk evaluation. In this work, phylogenetic analyses were performed using partial sequences of the translation elongation factor gene (EF-1a) of F. equiseti and F. acuminatum strains isolated from barley and wheat from Spain and other countries. The Northern and Southern European F. equiseti strains largely separated into two phylogenetically distinct clusters. This suggests the existence of two distinct populations within this species, explaining its presence in these regions of markedly different climate. Production of type A and B trichothecenes by the Spanish strains, examined in wheat cultures using a multitoxin analytical method, indicated that F. equiseti could produce deoxynivalenol and nivalenol and other trichothecenes, at concentrations that might represent a significant risk of toxin contamination for Southern European cereals. F. acuminatum showed low intraspecific genetic variability and 58% of the strains could produce deoxynivalenol at low level. Neither species was found to produce T-2 or HT-2 toxins. The present results provide important phylogenetic and toxigenic information essential for the accurate prediction of toxigenic risk.

Growth rate and TRI5 gene expression profiles of Fusarium equiseti strains isolated from Spanish cereals cultivated on wheat and barley media at different environmental conditions

Fusarium equiseti is a toxigenic species that often contaminates ce real crops from diverse climatic regions such as Northern and Southern Europe. Previous results suggested the existence of two distinct populations within this species with differences in toxin pro file which largely corresponded to North and South Europe (Spain). In this work, growth rate profiles of 4 F. equiseti strains isolated from different cereals and distinct Spanish regions were determined on wheat and barley based media at a range of temperatures (15, 20, 25, 30, 35 and 40 °C) and water potentialregimens(−0.7,−2.8,−7.0,and −9.8MPa,correspondingto 0.99,0.98,0.95 and 0.93aw values).Growth was observed at all temperatures except at 40 °C, and at all the solute potential values except at−9.8 MPa when combined with 15 °C. Optimal growth was observed at 20– 30 °C and −0.7/−2.8 MPa. The effect of these factors on trichothecene biosynthesis was examined on a F. equiseti strain using a newly developed real time RT-PCR protocol to quantify TRI5 gene expression at 15, 25 and 35 °C and −0.7, −2.8, − 7.0 and −9.8 MPa on wheat and barley based media. Induction of TRI5 expression was detected between 25 and 35 °C and −0.7 and − 2.8 MPa, with maximum values at 35 °C and −2.8 MPa being higher in barley than in wheat medium. These results appeared to be consistent with a population well adapted to the present climatic conditions and predicted scenarios for Southern Europe and suggested some differences depending on the cereal considered. These are also discussed in relation to other Fusarium species co-occurring in cereals grown in this region and to their significance for prediction and control strategies of toxigenic risk in future scenarios of climate change for this region.

Amino acid availability in ruminants of cereals and cereal co-products

Microbial corrected in situ estimatesof the ruminal undegraded fraction (RU) and intestinal effectivedigestibility (IED) of amino acids (AA), except tryptophan, of rye, wheat and corn grains, wheat bran, wheat and barley distilled dried grains and corn gluten feed were measured on three rumen- and duodenum-cannulated wethers using 15N-labelling techniques and considering ruminal rates of particle comminution and outflow.

Chroniques de Espanya [Texto impreso] : fins aci no divulgades : que tracta dels nobles e invictissims Reys dels Gots y gestes de aquells y dels Contes de Barcelona e Reys de Arago...

Copia digital: Biblioteca Valenciana, 2011

Coloqui nou en que Tito Bufalampolla y Sento el Formal conten la Maestransa que es feu ... este any 1789 en Valencia, y la Procesò de gracies ... ab motiu de haver pujàt al Trono de Espanya ... Don Carlos Quart ... : [Texto impreso] quarta y ultima part

Hay un ejemplar encuadernado con: Coloqui nou (XVIII/F-70).

Les defenses militars de la Guerra d'Espanya a Santa Pola

El capítulo inventaria y estudia los blocaos de defensa, los búnkeres y los asentamiento antiaéreos construidos por el ejército republicano hacia 1937-38 en el entorno de las playas desde el Tamarit al Carabassí y el cabo de Santa Pola, en los términos de Santa Pola y Elche (Alicante). Se trata de una primera aproximación para la puesta en valor de un patrimonio de arquitectura militar del siglo XX (de autoría anónima) que está degradándose y permanece olvidado, cuando no silenciado. Su recuperación es importante como monumento (documento) para que la historia no se vuelva a repetir. Se trata de un trabajo de inventariado, catalogación y valoración de piezas de arquitectura militar del siglo XX, como testigos de una época que no debe pasar al olvido. Se trata de un patrimonio que se inserta en las nueva sensibilidad ante el patrimonio surgido en la segunda mitad del s. XX: aquella que ensalza 'la vergüenza' y 'la calamidad' frente al heroísmo. Salvaguardar la 'memoria' para que los graves acontecimientos no se repitan.