源于G.I.Taylor工作的现代经典物理学

一位科学家的工作提供了一门完整课程的素材，涉及从流体动力学稳定性、湍流到流体电动力学、微生物的运动．

Starting Nozzle Flow Simulation Using K-G Two-Equation Turbulence Model

The starting process of two-dimensional nozzle flows has been simulated with Euler, laminar and k - g two-equation turbulence Navier-Stokes equations. The flow solver is based on a combination of LUSGS subiteration implicit method and five spatial discretized schemes, which are Roe, HLLE, MHLLE upwind schemes and AUSM+, AUSMPW schemes. In the paper, special attention is for the flow differences of the nozzle starting process obtained from different governing equations and different schemes. Two nozzle flows, previously investigated experimentally and numerically by other researchers, are chosen as our examples. The calculated results indicate the carbuncle phenomenon and unphysical oscillations appear more or less near a wall or behind strong shock wave except using HLLE scheme, and these unphysical phenomena become more seriously with the increase of Mach number. Comparing the turbulence calculation, inviscid solution cannot simulate the wall flow separation and the laminar solution shows some different flow characteristics in the regions of flow separation and near wall.

Histoire de Portugal : contenant les enterprises, navigations, & gestes memorables des Portugallois, tant en la cõqueste des Indes Orientales par eux descouvertes, qu'és guerres d'Afrique & autres exploits, depuis l'an mil quatre cens nonãte six, jusques à l'an mil cinq cens septante huit, sous Emmanuel premier, Jean troisieme, & Sebastian premier du nom / Pour Antoine Chuppin, ed. ; comprinse en vingt livres, dont les douze premiers sont traduits du latin de Ierosme Osorius, Euesque de Sylues en Algarve, les huit suivans prins de Lopez de Castagnede & d'autres historiensNouvellement mise en françois, par S.G.S. avec un discours du fruit qu'on peut recueillir de la lecture de cette histoire, & ample indice des matieres principales y contenues

Contém os empreendimentos, as navegações e os gestos memoráveis dos portugueses, inclusive em suas colônias.

Lagerversuch von frischem bzw. tief gefrorenen gelagerten kalt geräuchertem Atlantischem Lachs (Salmo salar) in 200-g-Fertigpackungen mit sensorischer Beurteilung in Abhängigkeit von der Lagerzeit

After 4 months frozen storage at –18 °C cold smoked Atlantic salmon in consumer packages can hardly be differentiated from the freshly smoked product by sensory assessment by an expert panel and cannot be differentiated by consumers.

Role of G protein coupled inwardly rectifier K+ channels (GIRK) on the neurobiology depression

353 págs.

液氮温区G-M制冷机的数值分析及新型结构制冷机的研制

G-M制冷机是回热式的小型低温制冷机，它利用绝热放气膨胀原理（又称为西蒙膨胀法）获得低温，具有振动小、运行稳定、寿命长、操作方便等特点，在对效率、重量、尺寸等没有太高要求的场合应用非常广泛。在八十年代末，G-M制冷机已经突破了液氦温度，非常适合在液氦温区为超导器件或电子元件提供冷量，在低温真空泵、MRI超导磁体冷却系统、SQUID再冷凝系统等方面有良好的应用前景，推广其应用已属当务之急。本文围绕着探索氦温区G-M制冷机工作机理、提高低温蓄冷器性能及新型结构G-M制冷机的研制等方面，进行了系统地理论分析和纳归纳，以及初步地实验研究：一. 首次比较完善地建立了液氦温区G-M型制冷机整机数值模拟方法，数值模拟方法给出了制冷机内工质参数瞬态分布及动态变化曲线，为分析制冷机独特的循环特性提供了直观的依据，为探讨运行及结构参数对制冷性能的影响机理提供了强有力的工具。模型中考虑了制冷机中的阻力、实际进排气角、物性变化及蓄冷器内空隙率的存在等多种实际因素：解决了一、二级耦合及部件交界处物性变化不连续对计算影响等问题；采取网格非均匀化、牛顿迭代法以及负反馈原理等措施，提高了计算精度和收敛性。在微机上成功地模拟了液氦温区G-M制冷机的工作过程，程序采用模块化编程，具有一定通用性。二. 运用液氦温区整套机数值模拟方法，计算了制冷机内工质参数（氦流温度、压力、流速等）周期性变化和空间分布，采用一级蓄冷器与低温蓄冷器工作特性对比法，从整机内工质参数动态变化规律分析的角度，验证了液氦温区G-M制冷机工质氦的循环主要分为两部分：常规循环、低温循环。并详细地讨论了运行参数（频率、工作压力）及一级制冷机结构参数对整机内工数动态变化的影响规律以及制冷机性能的影响机理。三. 首次建立了较完善的液氦温区多层混合填料型低温蓄冷器的数值模拟方法，运用数值模拟方法，首次详细地研究了常用填料的不同组合、一定组合下填料比例以及蓄冷器结构对制冷机性能的影响机理，提出了不同填料的最佳节组合及一定组合下填料最佳比例的确定和低温蓄冷器结构和填料优化的原则，为合理有效地设计高性能液氦温区低温蓄冷器提供了依据。四. 提出并设计、加工了一种新型结构的液氦温区双级G-M型制冷机，该机结构在国内外属于首创。其具有以下主要结构特点：一、二级分别独立驱动；一、二级之间通过热桥连接；二级蓄冷器外置于汽缸等。同时建立了新型结构液氦温区双级G-M型制冷机实验系统，为今后整机性能和内部动态过程的研究奠定了基础。五. 新型结构双级G-M型制冷机二级单机动转频率为0.6Hz时，制冷温度为13.6K，且在20K时有4.4W的制冷量；制冷机已达到液氦温区，f = 0.4Hz时，最低温度为4.6K；f = 1Hz，制冷温度为10K时，制冷量大于6W，上述结果目前均未见有文献报道。在新型G-M制冷机上，初步进行了低温蓄冷器性能测试实验及运行参数对制冷机性能影响的实验研究。

La gestión de las actividades musicales del G-9 en contextos de educación fornal y no formal

479 p.

Comparative growth and survival of fry of Clarias gariepinus (B), Clarias anguillaris (L), Heterobranchus bidorsalis (G), Heteroclarias, Clariabranchus and Clarias hybrids under outdoor nursery management system

Hybrids of Clariid catfishes; C. gariepinus (Netherlands), C. anguillaris, H. bidorsalis and their parental species were monitored for 8 weeks in 2 x 2 x 1m outdoor concrete tanks. The fry were fed NIFFR diet (40% crude protein) twice daily, 7 days of the week. Growth and survival records were taken weekly. The male HEB X female CLG hybrid showed an overall highest performance in growth rate while the lowest was recorded in male CLA X female CLG hybrid. The male HEB X female CLG hybrid grew at a faster rate than its reciprocal hybrid. In view or their growth rate, it is possible that the growth and survival rates or H. bidorsalis especially at the fry to fingerling stage could be improved through hybridization. The hybrid have potential as commercial food fish

Impact assessment of Niger State fisheries legislation on fisheries conservation in Edozhigi local government area (L.G.A.) of Niger State

A preliminary survey was conducted among the fishermen in five selected villages in Edozhigi L.G.A. of Niger State. One hundred and fifty fishermen were randomly selected and interviewed to find out the impact of Niger State fisheries legislation on fisheries conservation resources in the area. The analysis of data collected using descriptive statistics indicated that undersized mesh of gill nets, beach seines and traps are being used unabated. Also, fenced barriers across the entrance of flood plain ponds and Ex-bow lakes from the main stream are in the area. The fisheries rules and regulations implementers are rarely seen or not seen at all in the area. The decreasing nature of fish catches was detected. It is observed that government policy on fish conversation is neglected due to inadequate or lack of funding for meaningful extension and implementation of the fisheries rules and regulations

Sequence specific complexation of b DNA at sites containing g,c base pairs

A series of eight related analogs of distamycin A has been synthesized. Footprinting and affinity cleaving reveal that only two of the analogs, pyridine-2- car box amide-netropsin (2-Py N) and 1-methylimidazole-2-carboxamide-netrops in (2-ImN), bind to DNA with a specificity different from that of the parent compound. A new class of sites, represented by a TGACT sequence, is a strong site for 2-PyN binding, and the major recognition site for 2-ImN on DNA. Both compounds recognize the G•C bp specifically, although A's and T's in the site may be interchanged without penalty. Additional A•T bp outside the binding site increase the binding affinity. The compounds bind in the minor groove of the DNA sequence, but protect both grooves from dimethylsulfate. The binding evidence suggests that 2-PyN or 2-ImN binding induces a DNA conformational change.

In order to understand this sequence specific complexation better, the Ackers quantitative footprinting method for measuring individual site affinity constants has been extended to small molecules. MPE•Fe(II) cleavage reactions over a 10^5 range of free ligand concentrations are analyzed by gel electrophoresis. The decrease in cleavage is calculated by densitometry of a gel autoradiogram. The apparent fraction of DNA bound is then calculated from the amount of cleavage protection. The data is fitted to a theoretical curve using non-linear least squares techniques. Affinity constants at four individual sites are determined simultaneously. The distamycin A analog binds solely at A•T rich sites. Affinities range from 10^(6)- 10^(7)M^(-1) The data for parent compound D fit closely to a monomeric binding curve. 2-PyN binds both A•T sites and the TGTCA site with an apparent affinity constant of 10^(5) M^(-1). 2-ImN binds A•T sites with affinities less than 5 x 10^(4) M^(-1). The affinity of 2-ImN for the TGTCA site does not change significantly from the 2-PyN value. At the TGTCA site, the experimental data fit a dimeric binding curve better than a monomeric curve. Both 2-PyN and 2-ImN have substantially lower DNA affinities than closely related compounds.

In order to probe the requirements of this new binding site, fourteen other derivatives have been synthesized and tested. All compounds that recognize the TGTCA site have a heterocyclic aromatic nitrogen ortho to the N or C-terminal amide of the netropsin subunit. Specificity is strongly affected by the overall length of the small molecule. Only compounds that consist of at least three aromatic rings linked by amides exhibit TGTCA site binding. Specificity is only weakly altered by substitution on the pyridine ring, which correlates best with steric factors. A model is proposed for TGTCA site binding that has as its key feature hydrogen bonding to both G's by the small molecule. The specificity is determined by the sequence dependence of the distance between G's.

One derivative of 2-PyN exhibits pH dependent sequence specificity. At low pH, 4-dimethylaminopyridine-2-carboxamide-netropsin binds tightly to A•T sites. At high pH, 4-Me_(2)NPyN binds most tightly to the TGTCA site. In aqueous solution, this compound protonates at the pyridine nitrogen at pH 6. Thus presence of the protonated form correlates with A•T specificity.

The binding site of a class of eukaryotic transcriptional activators typified by yeast protein GCN4 and the mammalian oncogene Jun contains a strong 2-ImN binding site. Specificity requirements for the protein and small molecule are similar. GCN4 and 2-lmN bind simultaneously to the same binding site. GCN4 alters the cleavage pattern of 2-ImN-EDTA derivative at only one of its binding sites. The details of the interaction suggest that GCN4 alters the conformation of an AAAAAAA sequence adjacent to its binding site. The presence of a yeast counterpart to Jun partially blocks 2-lmN binding. The differences do not appear to be caused by direct interactions between 2-lmN and the proteins, but by induced conformational changes in the DNA protein complex. It is likely that the observed differences in complexation are involved in the varying sequence specificity of these proteins.