Ultrastructural study of spermiogenesis and the spermatozoon of the proteocephalidean cestode Barsonella lafoni de Chambrier et al., 2009, a parasite of the catfish Clarias gariepinus (Burchell, 1822) (Siluriformes, Clariidae)

Spermiogenesis in the proteocephalidean cestode Barsonella lafoni de Chambrier et al., 2009 shows typical characteristics of the type I spermiogenesis. These include the formation of distal cytoplasmic protrusions forming the differentiation zones, lined by cortical microtubules and containing two centrioles. An electron-dense material is present in the apical region of the differentiation zone during the early stages of spermiogenesis. Each centriole is associated to a striated rootlet, being separated by an intercentriolar body. Two free and unequal flagella originate from the centrioles and develop on the lateral sides of the differentiation zone. A median cytoplasmic process is formed between the flagella. Later these flagella rotate, become parallel to the median cytoplasmic process and finally fuse proximodistally with the latter. It is interesting to note that both flagellar growth and rotation are asynchronous. Later, the nucleus enlarges and penetrates into the spermatid body. Finally, the ring of arching membranes is strangled and the young spermatozoon is detached from the residual cytoplasm. The mature spermatozoon presents two axonemes of the 9 +"1" trepaxonematan pattern, crested body, parallel nucleus and cortical microtubules, and glycogen granules. Thus, it corresponds to the type II spermatozoon, described in almost all Proteocephalidea. The anterior extremity of the gamete is characterized by the presence of an apical cone surrounded by the lateral projections of the crested body. An arc formed by some thick and parallel cortical microtubules appears at the level of the centriole. They surround the centriole and later the first axoneme. This arc of electron-dense microtubules disorganizes when the second axoneme appears, and then two parallel rows of thin cortical microtubules are observed. The posterior extremity of the male gamete exhibits some cortical microtubules. This type of posterior extremity has never been described in proteocephalidean cestodes. The ultrastructural features of the spermatozoon/spermiogenesis of the Proteocephalidea species are analyzed and compared.

Paleoceanography of sediment cores SO202-27-6 and MD01-2416

The glacial-to-Holocene evolution of subarctic Pacific surface water stratification and silicic acid (Si) dynamics is investigated based on new combined diatom oxygen (d18Odiat) and silicon (d30Sidiat) isotope records, along with new biogenic opal, subsurface foraminiferal d18O, alkenone-based sea surface temperature, sea ice, diatom, and core logging data from the NE Pacific. Our results suggest that d18Odiat values are primarily influenced by changes in freshwater discharge from the Cordilleran Ice Sheet (CIS), while corresponding d30Sidiat are primarily influenced by changes in Si supply to surface waters. Our data indicate enhanced glacial to mid Heinrich Stadial 1 (HS1) NE Pacific surface water stratification, generally limiting the Si supply to surface waters. However, we suggest that an increase in Si supply during early HS1, when surface waters were still stratified, is linked to increased North Pacific Intermediate Water formation. The coincidence between fresh surface waters during HS1 and enhanced ice-rafted debris sedimentation in the North Atlantic indicates a close link between CIS and Laurentide Ice Sheet dynamics and a dominant atmospheric control on CIS deglaciation. The Bølling/Allerød (B/A) is characterized by destratification in the subarctic Pacific and an increased supply of saline, Si-rich waters to surface waters. This change toward increased convection occurred prior to the Bølling warming and is likely triggered by a switch to sea ice-free conditions during late HS1. Our results furthermore indicate a decreased efficiency of the biological pump during late HS1 and the B/A (possibly also the Younger Dryas), suggesting that the subarctic Pacific has then been a source region of atmospheric CO2.

Age determination and model of sediment core RAPiD-15-4P

Greenland ice core records indicate that the last deglaciation (~7-21 ka) was punctuated by numerous abrupt climate reversals involving temperature changes of up to 5°C-10°C within decades. However, the cause behind many of these events is uncertain. A likely candidate may have been the input of deglacial meltwater, from the Laurentide ice sheet (LIS), to the high-latitude North Atlantic, which disrupted ocean circulation and triggered cooling. Yet the direct evidence of meltwater input for many of these events has so far remained undetected. In this study, we use the geochemistry (paired Mg/Ca-d18O) of planktonic foraminifera from a sediment core south of Iceland to reconstruct the input of freshwater to the northern North Atlantic during abrupt deglacial climate change. Our record can be placed on the same timescale as ice cores and therefore provides a direct comparison between the timing of freshwater input and climate variability. Meltwater events coincide with the onset of numerous cold intervals, including the Older Dryas (14.0 ka), two events during the Allerød (at ~13.1 and 13.6 ka), the Younger Dryas (12.9 ka), and the 8.2 ka event, supporting a causal link between these abrupt climate changes and meltwater input. During the Bølling-Allerød warm interval, we find that periods of warming are associated with an increased meltwater flux to the northern North Atlantic, which in turn induces abrupt cooling, a cessation in meltwater input, and eventual climate recovery. This implies that feedback between climate and meltwater input produced a highly variable climate. A comparison to published data sets suggests that this feedback likely included fluctuations in the southern margin of the LIS causing rerouting of LIS meltwater between southern and eastern drainage outlets, as proposed by Clark et al. (2001, doi:10.1126/science.1062517).

Age determination and Heinrich Events in sediment cores SU90-08 and KET80-03

The Mediterranean Sea hydrology at the time of the Heinrich formation in the North Atlantic Ocean was analyzed by comparing sea surface temperatures (SSTs) and oxygen isotope composition of seawater (dw) changes during the past 75 kyr in two marine cores. These were compared to the palynological variations derived in the Mediterranean Sea core. During the last glacial the two oceanic SST records show similar and synchronous patterns, with several long-term cooling periods, ending by abrupt SST increases. At the time of the Heinrich events, cold SSTs and low salinity prevailed in the Mediterranean Sea. The freshwater budget was similar to the modern one, permitting the presence of a mixed forest on the Mediterranean borderlands. The post-Heinrich periods are marked by a freshwater budget decrease, limiting oak and fir tree growth in the Mediterranean region. Increase of precipitation or reduction of evaporation is observed before the Heinrich episode, and is associated with a well-developed mixed Mediterranean forest.

Pedogeomorphology, geochronology and paleobotany of soil profiles obtained in the Kyichu River catchment, sourthern Tibet

Results of pedogeomorphological, geochronological and paleobotanical investigations are presented covering the last ca. 4,000 years. The study sites are located in the heavily degraded Kyichu River catchment around Lhasa at 3,600-4,600 m a.s.l. Repeatedly, colluvial sediments have been recorded overlying paleosols. These deposits can be divided into i) coarse-grained sediments with a high proportion of stones and boulders originating from alluvial fans and debris flows, ii) matrix supported sediments with some stones and boulders originating from mudflows or combined colluvial processes such as hillwash plus rock fall, and iii) fine-grained sediments originating from hill wash. The IRSL multi-level dating of profile QUG 1 points to a short-time colluvial sedimentation between 1.0 ± 0.1 and 0.8 ± 0.1 ka. In contrast, dated paleosols of profile GAR 1 (7,908 ± 99 and 3,668 ± 57 BP) encompass a first colluvial episode. Here, the upper colluvial sedimentation took place during several periods between 2.6 ± 0.3 and 0.4 ± 0.1 ka. For the first time in Tibet, a systematic extraction, determination and dating of charcoals from buried paleosols was conducted. The charcoals confirm the Late Holocene presence of juniper forests or woodlands in a now treeless, barren environment. A pollen diagram from Lhasa shows a distinct decline of pollen of the Jumperus-type around 4,140 ± 50 BP, which is interpreted as indicating a clearing of forests on the adjacent slopes. It is assumed that the environmental changes from forests to desertic rangelands since ca. 4,000 BP have been at least reinforced by humans.

Water chemistry and sediment age of lake Terrasovoje, Amery Oasis, East Antarctica

The late Quaternary palaeoenvironmental history of the southern Windmill Islands, East Antarctica, has been reconstructed using diatom assemblages from two long, well-dated sediment cores taken in two marine bays. The diatom assemblage of the lowest sediment layers suggests a warm climate with mostly open water conditions during the late Pleistocene. During the following glacial, the Windmill Islands were covered by grounded ice preventing any in situ bioproductivity. Following deglaciation, a sapropel with a well-preserved diatom assemblage was deposited from ~10?500 cal yr BP. Between ~10?500 and ~4000 cal yr BP, total organic carbon (Corg) and total diatom valve concentrations as well as the diatom species composition suggest relatively cool summer temperatures. Hydrological conditions in coastal bays were characterised by combined winter sea-ice and open water conditions. This extensive period of glacial retreat was followed by the Holocene optimum (~4000 to ~1000 cal yr BP), which occurred later in the southern Windmill Islands than in most other Antarctic coastal regions. Diatom assemblages in this period suggest ice-free conditions and meltwater-stratified waters in the marine bays during summer, which is also reflected in high proportions of freshwater diatoms in the sediments. The diatom assemblage in the upper sediments of both cores indicates Neoglacial cooling from ~1000 cal yr BP, which again led to seasonally persistent sea-ice on the bays. The Holocene optimum and cooling trends in the Windmill Islands did not occur contemporaneously with other Antarctic coastal regions, showing that the here presented record reflects partly local environmental conditions rather than global climatic trends.

Water chemistry, sediment radiocarbon age and diatom zonation at Peterson Inlet and Browning Bay, Antarctica

The late Quaternary palaeoenvironmental history of the southern Windmill Islands, East Antarctica, has been reconstructed using diatom assemblages from two long, well-dated sediment cores taken in two marine bays. The diatom assemblage of the lowest sediment layers suggests a warm climate with mostly open water conditions during the late Pleistocene. During the following glacial, the Windmill Islands were covered by grounded ice preventing any in situ bioproductivity. Following deglaciation, a sapropel with a well-preserved diatom assemblage was deposited from ~10500 cal yr BP. Between ~10500 and ~4000 cal yr BP, total organic carbon (Corg) and total diatom valve concentrations as well as the diatom species composition suggest relatively cool summer temperatures. Hydrological conditions in coastal bays were characterised by combined winter sea-ice and open water conditions. This extensive period of glacial retreat was followed by the Holocene optimum (~4000 to ~1000 cal yr BP), which occurred later in the southern Windmill Islands than in most other Antarctic coastal regions. Diatom assemblages in this period suggest ice-free conditions and meltwater-stratified waters in the marine bays during summer, which is also reflected in high proportions of freshwater diatoms in the sediments. The diatom assemblage in the upper sediments of both cores indicates Neoglacial cooling from ~1000 cal yr BP, which again led to seasonally persistent sea-ice on the bays. The Holocene optimum and cooling trends in the Windmill Islands did not occur contemporaneously with other Antarctic coastal regions, showing that the here presented record reflects partly local environmental conditions rather than global climatic trends.

Pedology, geochronology, palynology and macrofossils of soil profiles in the Altdarss area, german Baltic Sea coast

A new site with Lateglacial palaeosols covered by 0.8 - 2.4 m thick aeolian sands is presented. The buried soils were subjected to multidisciplinary analyses (pedology, micromorphology, geochronology, dendrology, palynology, macrofossils). The buried soil cover comprises a catena from relatively dry ('Nano'-Podzol, Arenosol) via moist (Histic Gleysol, Gleysol) to wet conditions (Histosol). Dry soils are similar to the so-called Usselo soil, as described from sites in NW Europe and central Poland. The buried soil surface covers ca. 3.4 km**2. Pollen analyses date this surface into the late Aller0d. Due to a possible contamination by younger carbon, radiocarbon dates are too young. OSL dates indicate that the covering by aeolian sands most probably occurred during the Younger Dryas. Botanical analyses enables the reconstruction of a vegetation pattern typical for the late Allerod. Large wooden remains of pine and birch were recorded.

Age determination and median grain size of sediment core EN31-PC2

Hemipelagic muds deposited during the past 5.3 cal kyr in the northern Gulf of Mexico (Orca Basin) contain seven intervals punctuated by relatively coarse siliciclastic grain-size peaks, planktonic faunal turnovers, and negative d13C excursions. We believe these episodes represent megaflood deposits reflecting historically unprecedented outfall of North American floodwater and terrigenous mud plumes into the gulf, resulting in collapse of the open-ocean pelagic ecosystem. The deposits record multidecadal episodes of high continental precipitation and large Mississippi River floods at ~4.7, 3.5, 3.0, 2.5, 2.0, 1.2, and 0.3 cal ka (500-1200-year recurrence interval). Variations in tropical plankton frequencies define submillenial warming intervals that culminate in these fluvial episodes. Strengthened tropical currents in the gulf at these times appear to have increased sea surface temperatures and associated flow of moist gulf air to the midwest. Terrestrial paleohydrologic records support the marine evidence for millennial-scale changes in recurrence of large midwest flood episodes.

High-resolution sedimentological and paleontological record of ODP Site 167-1019

Holocene and latest Pleistocene oceanographic conditions and the coastal climate of northern California have varied greatly, based upon high-resolution studies (ca. every 100 years) of diatoms, alkenones, pollen, CaCO3%, and total organic carbon at Ocean Drilling Program (ODP) Site 1019 (41.682°N, 124.930°W, 980 m water depth). Marine climate proxies (alkenone sea surface temperatures [SSTs] and CaCO3%) behaved remarkably like the Greenland Ice Sheet Project (GISP)-2 oxygen isotope record during the Bølling-Allerod, Younger Dryas (YD), and early part of the Holocene. During the YD, alkenone SSTs decreased by >3°C below mean Bølling-Allerod and Holocene SSTs. The early Holocene (ca. 11.6 to 8.2 ka) was a time of generally warm conditions and moderate CaCO3 content (generally >4%). The middle part of the Holocene (ca. 8.2 to 3.2 ka) was marked by alkenone SSTs that were consistently 1-2°C cooler than either the earlier or later parts of the Holocene, by greatly reduced numbers of the gyre-diatom Pseudoeunotia doliolus (<10%), and by a permanent drop in CaCO3% to <3%. Starting at ca. 5.2 ka, coastal redwood and alder began a steady rise, arguing for increasing effective moisture and the development of the north coast temperate rain forest. At ca. 3.2 ka, a permanent ca. 1°C increase in alkenone SST and a threefold increase in P. doliolus signaled a warming of fall and winter SSTs. Intensified (higher amplitude and more frequent) cycles of pine pollen alternating with increased alder and redwood pollen are evidence that rapid changes in effective moisture and seasonal temperature (enhanced El Niño-Southern Oscillation [ENSO] cycles) have characterized the Site 1019 record since about 3.5 ka.

Oxygen isotope and Mg/Ca record of planktonic foraminifera of sediment core MD01-2390

Changes in the local freshwater budget over the last 22,000 years have been estimated from a sediment core located in the southern South China Sea (SCS) using a combined approach of Mg/Ca and oxygen isotopes on the planktonic foraminifera Globigerinoides ruber (white) sensu stricto (s.s.). Core MD01-2390 (06°28,12N, 113°24,56E; water depth 1591 m) is located near the glacial paleo-river mouths of the Baram, Rajang and North Sunda/Molengraaff Rivers that drained the exposed Sunda Shelf. The delta18Oseawater record reveals lower average values (-0.96±0.18 per mil) during the Last Glacial Maximum (LGM) when compared with modern values (-0.54±0.18 per mil). Low salinity during the LGM is interpreted to reflect a higher freshwater contribution due to a greater proximity of the core site to the mouths of the Baram, Rajang and North Sunda/Molengraaff Rivers at that time. A general deglacial increasing trend in salinity due to the progressive landward displacement of the coastline during deglacial shelf flooding is punctuated by several short-term shifts towards higher and lower salinity that are likely related to abrupt changes in the intensity of the East Asian summer monsoon. Thus, the deglacial delta18Oseawater changes reflect the combined effects of sea-level-induced environmental changes on the shelf (e.g. phases of retreat and breakdown of the shelf drainage systems) and East Asian monsoon climate change. Lower salinity than at present during the Early Holocene may be attributed to an increase in summer monsoonal precipitation that is corroborated by previous marine and terrestrial studies that report a Preboreal-Early Holocene monsoon optimum in the Asian monsoon region.