An animal model for the study of Chamomilla in stress and depression: pilot study

The behavioral and hematological effects of treatment with Chamomilla 6cH in mice subjected to experimental stress are described. Swiss mice were randomly divided into pairs, one animal was inoculated with Ehrlich's tumor, the other was treated daily with Chamomilla 6cH or control or received no treatment. After 7 days, the animals were observed in an open-field arena and blood samples taken. Mice who cohabitated with a sick cage-mate showed a decrease in their general activity, but those treated with Chamomilla 6cH were less severely affected (p = 0.0426). No hematologicall changes were observed.In a second experiment, the forced swimming test was applied to mice pre-treated with Chamomilla 6cH, controls were: water, 10% ethanol or amitriptyline. Only the amitriptyline and ethanol treated groups showed significant excitatory behavior (p = 0.0020), Chamomilla 6cH treated animals' scores intermediate between water control and ethanol or amitriptyline. A decrease in the leukocyte count was observed in the amitriptyline and Chamomilla 6cH treated groups (p = 0.039). These data suggest that treatment with Chamomilla 6cH is related to the recovery of basal behavioral conditions in mice subjected to stressful conditions.

Prevention of postnatal distress or depression : an evaluation of an intervention at preparation for parenthood classes.

Determined the effectiveness of a psychosocial intervention, provided to expectant couples in routine antenatal classes, on the postpartum psychosocial adjustment of women and men. Preparation for Parenthood programs were randomly allocated to one of three conditions: usual service ('control'), experimental ('empathy'), or non-specific control ('baby-play'). The latter condition controlled for the non-specific effects of the intervention, these being: the provision of an extra class; asking couples to consider the early postpartum weeks; and receiving booster information after the antenatal class, and again shortly after the birth. Women and men were categorised into three levels of self-esteem, as measured antenatally: low, medium and high. 268 participants were recruited antenatally. Interview data and self-report information was collected from 202 of these women at 6 weeks postpartum, and 180 women at 6 months postpartum. The intervention consisted of a session focusing on psychosocial issues related to becoming first-time parents. Participants discussed possible postpartum concerns in separate gender groups for part of the session, and then discussed these issues with their partners

Stress and depression in informal caregivers of patients with bipolar affective disorder

This study aims to establish the stress and depression´s prevalence in informal primary caregivers of patients with bipolar affective disorder of the Clínica de Nuestra Señora de la Paz (Bogotá, Colombia). The sample consisted of 40 informal primary caregivers who were tested by several tools: a survey filter, a sociodemographic record, the Beck Depression Inventory (BDI) and the Daily Stress Questionnaire. Results indicate that there is much more presence of depression than of daily stress in the sample. 

Supplementation with fruit and vegetable soups and beverages increases plasma carotenoid concentrations but does not alter markers of oxidative stress or cardiovascular risk factors

This study was aimed at determining whether an increase of 5 portions of fruits and vegetables in the form of soups and beverages has a beneficial effect on markers of oxidative stress and cardiovascular disease risk factors. The study was a single blind, randomized, controlled, crossover dietary intervention study. After a 2-wk run-in period with fish oil supplementation, which continued throughout the dietary intervention to increase oxidative stress, the volunteers consumed carotenoid-rich or control vegetable soups and beverages for 4 wk. After a 10-wk wash-out period, the volunteers repeated the above protocol, consuming the other intervention foods. Both test and control interventions significantly increased the % energy from carbohydrates and decreased dietary protein and vitamin B-12 intakes. Compared with the control treatment, consumption of the carotenoid-rich soups and beverages increased dietary carotenoids, vitamin C, alpha-tocopherol, potassium, and folate, and the plasma concentrations of alpha-carotene (362%), beta-carotene (250%) and lycopene (31%) (P < 0.01) and decreased the plasma homocysteine concentration by 8.8% (P < 0.01). The reduction in plasma homocysteine correlated weakly with the increase in dietary folate during the test intervention (r = -0.35, P = 0.04). The plasma antioxidant status and markers of oxidative stress were not affected by treatment. Consumption of fruit and vegetable soups and beverages makes a useful contribution to meeting dietary recommendations for fruit and vegetable consumption.

Effect of heat stress or lipopolysaccharide (E-coli) injection on HSP70 levels in the liver and brain of adrenalectomized rats

Hsp70 content (ng Hsp70 mu g total protein(-1)) in the liver and brain of control and adrenalectomized male rats was investigated by Western Blotting after heat stress (40 degrees C) or endotoxin-induced fever (E. coli lipopolysaccharide injection). The increase in rectal temperature was higher after heat stress than after LPS injection, Heat stress affected Hsp70 content of the liver, but not of the brain; however adrenalectomy did not influence any parameter. These results suggest that, under these circumstances, there is no relationship between the hypothalamic-pituitary-adrenal axis and Hsp70 synthesis in liver and brain. (C) 2000 Elsevier B.V. Ltd. All rights reserved.

Interpersonal Trauma, Posttraumatic Stress and Depression

Dating aggression is common among emerging adults, and women who experience aggression from a dating partner are at risk for elevated depression and posttraumatic stress (Dutton et al., 2006). Although some women end their relationships as a result of aggression, other women remain committed to their partner, and aggression tends to escalate over time. The current study explored the role that depression and posttraumatic stress play in ending aggressive dating relationships as well as changes in these symptoms after ending such a relationship. The current study also sought to identify factors predictive of individual differences in emerging adults' commitment to their aggressive dating relationships. A sample of 148 emerging adult women currently in an aggressive dating relationship completed questionnaires about themselves and their relationship; measures of rejection sensitivity, self-worth, and romantic relational style were included as predictors of the Investment Model variables (e.g., investment, satisfaction, quality of alternatives, and commitment; Rusbult, 1980). Two assessments were completed six months apart. Neither depression nor posttraumatic stress predicted ending an aggressive relationship. However, ending an aggressive relationship was associated with experiencing less physical aggression, which mediated reductions in posttraumatic stress. A more avoidant romantic style indirectly predicted commitment through relationship satisfaction and investment. Both commitment and rejection sensitivity significantly predicted continuing an aggressive relationship six months later.

Lost in translation? The potential psychobiotic Lactobacillus rhamnosus (JB-1) fails to modulate stress or cognitive performance in healthy male subjects

Background: Preclinical studies have identified certain probiotics as psychobiotics a live microorganisms with a potential mental health benefit. Lactobacillus rhamnosus (JB-1) has been shown to reduce stress-related behaviour, corticosterone release and alter central expression of GABA receptors in an anxious mouse strain. However, it is unclear if this single putative psychobiotic strain has psychotropic activity in humans. Consequently, we aimed to examine if these promising preclinical findings could be translated to healthy human volunteers. Objectives: To determine the impact of L. rhamnosus on stress-related behaviours, physiology, inflammatory response, cognitive performance and brain activity patterns in healthy male participants. An 8 week, randomized, placebo-controlled, cross-over design was employed. Twenty-nine healthy male volunteers participated. Participants completed self-report stress measures, cognitive assessments and resting electroencephalography (EEG). Plasma IL10, IL1β, IL6, IL8 and TNFα levels and whole blood Toll-like 4 (TLR-4) agonist-induced cytokine release were determined by multiplex ELISA. Salivary cortisol was determined by ELISA and subjective stress measures were assessed before, during and after a socially evaluated cold pressor test (SECPT). Results: There was no overall effect of probiotic treatment on measures of mood, anxiety, stress or sleep quality and no significant effect of probiotic over placebo on subjective stress measures, or the HPA response to the SECPT. Visuospatial memory performance, attention switching, rapid visual information processing, emotion recognition and associated EEG measures did not show improvement over placebo. No significant anti-inflammatory effects were seen as assessed by basal and stimulated cytokine levels. Conclusions: L. rhamnosus was not superior to placebo in modifying stress-related measures, HPA response, inflammation or cognitive performance in healthy male participants. These findings highlight the challenges associated with moving promising preclinical studies, conducted in an anxious mouse strain, to healthy human participants. Future interventional studies investigating the effect of this psychobiotic in populations with stress-related disorders are required.

Anxiety, stress and depression in young adults with type 1 Diabetes

Introdução: A vida dos jovens adultos com diabetes tipo 1 (DM1) tem muitas exigências e as consequências psicológicas da adesão contínua aos aspetos do tratamento pode afetar a qualidade de vida. Objetivos: Conhecer o suporte social, satisfação com a vida, ansiedade, stresse e depressão nos jovens adultos com DM1. Material e Métodos: Estudo quantitativo realizado com 278 jovens adultos com DM1 (18 - 35 anos). Resultados: Os jovens consideram ter bom suporte social. A média de satisfação com a vida é 6,6 ±1,7 (escala 0-10). A maior parte dos jovens não apresenta estados persistentes de ansiedade e de excitação e tensão (stresse), pelo que têm resistência à frustração e desilusão. A maioria dos jovens não apresenta sintomas de depressão, revelando auto-estima, sentimentos positivos, motivação, entusiasmo e perceção da probabilidade de alcançar objetivos de vida que sejam significativos. A análise fatorial das escalas de ansiedade, stresse e depressão permitiu encontrar 3 fatores que explicam 50% da variância total: stresse (36%), ansiedade (8%), depressão (6%). Conclusões: Os jovens adultos com DM1 têm bom suporte social e satisfação com a vida. A maior parte dos jovens não revela sintomas de ansiedade, stresse e depressão. O suporte social e a satisfação com a vida poderão contribuir para uma boa saúde mental.

OS BENEFÍCIOS DO MÉTODO CANGURU PARA OS PAIS DE RECÉM-NASCIDOS PREMATUROS INTERNADOS NUMA UCIN

Introdução O nascimento de um bebé prematuro pode ter efeitos adversos na saúde mental dos pais. As dificuldades emocionais são transversais a todos os pais durante o internamento do bebé numa UCIN (Diaz, Fernandes & Correia (2014). Este internamento é um acontecimento stressante, pois a separação pais/bebé prejudica o processo de adaptação à parentalidade. Atualmente nas UCIN's é colocado em prática o método canguru, com relatos de benefícios (Morelius, Ortenstrand, Theodorsson & Frostell, 2014), mas estas evidências não estão suficientemente evidenciadas. Objetivos O método canguru é uma intervenção que consiste no contacto pele-a-pele entre a mãe ou pai, e o recém-nascido, promovendo o contacto e ligação. Embora utilizado em algumas unidades, carece de maior generalização sendo que a síntese de evidências acerca dos benefícios potenciará esta intervenção. Neste contexto, o objetivo central desta investigação consistiu em sintetizar os principais benefícios do método canguru para os pais de recém-nascidos prematuros internados numa UCIN. Metodologia Foi efetuada uma revisão integrativa da literatura. Para tal, foi realizada pesquisa nas bases de dados online: Medline With Full Text; Cinahl plus with full text; MedicLatina; Academic Search Complete. A expressão de pesquisa selecionada foi a seguinte: parent* stress OR depression or anxiety AND kangaroo care AND premature OR preterm. Através da estratégia PICOD, elaborámos uma questão de pesquisa e definimos os critérios de inclusão e exclusão. Após, leitura de títulos, abstract, e leitura integral, constituíram a amostra final nove artigos, com os quais respondemos à questão de investigação. Resultados Há evidências de que o método canguru tem benefícios para a díade e para o casal em todo o processo de adaptação à parentalidade e à ligação com o bebé, particularmente importante também por ser uma situação de risco. De acordo com a análise dos artigos que constituíram a mostra registamos como significativo que: Em três estudos esta experiência foi vivenciada como experiência calmante e positiva, que favorece a ligação mãe-filho. Noutro estudo, as mães manifestaram sentimentos de utilidade nos cuidados ao filho. Noutro estudo especialmente as mães expressaram um aumento de confiança, ajudando ao conhecimento das particularidades do bebé. Outros estudos concluíram da redução da incidência da depressão pós-parto ou uma melhoria da depressão pós-parto, se instalada. Noutro estudo foi verificado um efeito positivo nos problemas conjugais quando aplicado por ambos os elementos do casal e um maior entendimento e suporte entre os mesmos. Conclusões Podemos concluir que é positivo o uso do método canguru nas unidades de cuidados intensivos neonatais com bebés prematuros. Há evidências de que pode atenuar as dificuldades sentidas pelos pais durante o internamento, promovendo maior confiança e adaptação ao seu papel. A redução do stress parental, particularmente da ansiedade materna, torna de facto mais ajustada a presença, envolvimento e parceria neste contexto. Este quadro de bebé de risco potencia a depressão pós-parto mas esta pode melhorar com o uso deste método. Em suma é uma intervenção promotora da vinculação fazendo emergir nos pais um sentimento de maior felicidade.

Os benefícios do método canguru para os pais de recém-nascidos prematuros, internados numa UCIN

Introdução O nascimento de um bebé prematuro pode ter efeitos adversos na saúde mental dos pais. As dificuldades emocionais são transversais a todos os pais durante o internamento do bebé numa UCIN (Diaz, Fernandes & Correia (2014). Este internamento é um acontecimento stressante, pois a separação pais/bebé prejudica o processo de adaptação à parentalidade. Atualmente nas UCIN's é colocado em prática o método canguru, com relatos de benefícios (Morelius, Ortenstrand, Theodorsson & Frostell, 2014), mas estas evidências não estão suficientemente evidenciadas. Objetivos O método canguru é uma intervenção que consiste no contacto pele-a-pele entre a mãe ou pai, e o recém-nascido, promovendo o contacto e ligação. Embora utilizado em algumas unidades, carece de maior generalização sendo que a síntese de evidências acerca dos benefícios potenciará esta intervenção. Neste contexto, o objetivo central desta investigação consistiu em sintetizar os principais benefícios do método canguru para os pais de recém-nascidos prematuros internados numa UCIN. Metodologia Foi efetuada uma revisão integrativa da literatura. Para tal, foi realizada pesquisa nas bases de dados online: Medline With Full Text; Cinahl plus with full text; MedicLatina; Academic Search Complete. A expressão de pesquisa selecionada foi a seguinte: parent* stress OR depression or anxiety AND kangaroo care AND premature OR preterm. Através da estratégia PICOD, elaborámos uma questão de pesquisa e definimos os critérios de inclusão e exclusão. Após, leitura de títulos, abstract, e leitura integral, constituíram a amostra final nove artigos, com os quais respondemos à questão de investigação. Resultados Há evidências de que o método canguru tem benefícios para a díade e para o casal em todo o processo de adaptação à parentalidade e à ligação com o bebé, particularmente importante também por ser uma situação de risco. De acordo com a análise dos artigos que constituíram a mostra registamos como significativo que: Em três estudos esta experiência foi vivenciada como experiência calmante e positiva, que favorece a ligação mãe-filho. Noutro estudo, as mães manifestaram sentimentos de utilidade nos cuidados ao filho. Noutro estudo especialmente as mães expressaram um aumento de confiança, ajudando ao conhecimento das particularidades do bebé. Outros estudos concluíram da redução da incidência da depressão pós-parto ou uma melhoria da depressão pós-parto, se instalada. Noutro estudo foi verificado um efeito positivo nos problemas conjugais quando aplicado por ambos os elementos do casal e um maior entendimento e suporte entre os mesmos. Conclusões Podemos concluir que é positivo o uso do método canguru nas unidades de cuidados intensivos neonatais com bebés prematuros. Há evidências de que pode atenuar as dificuldades sentidas pelos pais durante o internamento, promovendo maior confiança e adaptação ao seu papel. A redução do stress parental, particularmente da ansiedade materna, torna de facto mais ajustada a presença, envolvimento e parceria neste contexto. Este quadro de bebé de risco potencia a depressão pós-parto mas esta pode melhorar com o uso deste método. Em suma é uma intervenção promotora da vinculação fazendo emergir nos pais um sentimento de maior felicidade.

What explains the association between socioeconomic status and depression among Vietnamese adults?

Background Depression is a major public health problem worldwide and is currently ranked second to heart disease for years lost due to disability. For many decades, international research has found that depressive symptoms occur more frequently among low socioeconomic (SES) individuals than their more-advantaged peers. However, the reasons as to why those of low socioeconomic groups suffer more depressive symptoms are not well understood. Studies investigating the prevalence of depression and its association with SES emanate largely from developed countries, with little research among developing countries. In particular, there is a serious dearth of research on depression and no investigation of its association with SES in Vietnam. The aims of the research presented in this Thesis are to: estimate the prevalence of depressive symptoms among Vietnamese adults, examine the nature and extent of the association between SES and depression and to elucidate causal pathways linking SES to depressive symptoms Methods The research was conducted between September 2008 and November 2009 in Hue city in central Vietnam and used a combination of qualitative (in-depth interviews) and quantitative (survey) data collection methods. The qualitative study contributed to the development of the theoretical model and to the refinement of culturally-appropriate data collection instruments for the quantitative study. The main survey comprised a cross-sectional population–based survey with randomised cluster sampling. A sample of 1976 respondents aged between 25-55 years from ten randomly-selected residential zones (quarters) of Hue city completed the questionnaire (response rate 95.5%). Measures SES was classified using three indicators: education, occupation and income. The Center for Epidemiologic Studies-Depression (CES-D) scale was used to measure depressive symptoms (range0-51, mean=11.0, SD=8.5). Three cut-off points for the CES-D scores were applied: ‘at risk for clinical depression’ (16 or above), ‘depressive symptoms’ (above 21) and ‘depression’ (above 25). Six psychosocial indicators: life time trauma, chronic stress, recent life events, social support, self esteem, and mastery were hypothesized to mediate the association between SES and depressive symptoms. Analyses The prevalence of depressive symptoms were analysed using bivariate analyses. The multivariable analytic phase comprised of ordinary least squares regression, in accordance with Baron and Kenny’s three-step framework for mediation modeling. All analyses were adjusted for a range of confounders, including age, marital status, smoking, drinking and chronic diseases and the mediation models were stratified by gender. Results Among these Vietnamese adults, 24.3% were at or above the cut-off for being ‘at risk for clinical depression’, 11.9% were classified as having depressive symptoms and 6.8% were categorised as having depression. SES was inversely related to depressive symptoms: the least educated those with low occupational status or with the lowest incomes reported more depressive symptoms. Socioeconomicallydisadvantaged individuals were more likely to report experiencing stress (life time trauma, chronic stress or recent life events), perceived less social support and reported fewer personal resources (self esteem and mastery) than their moreadvantaged counterparts. These psychosocial resources were all significantly associated with depressive symptoms independent of SES. Each psychosocial factor showed a significant mediating effect on the association between SES and depressive symptoms. This was found for all measures of SES, and for males and females. In particular, personal resources (mastery, self esteem) and chronic stress accounted for a substantial proportion of the variation in depressive symptoms between socioeconomic groups. Social support and recent life events contributed modestly to socioeconomic differences in depressive symptoms, whereas lifetime trauma contributed the least to these inequalities. Conclusion This is the first known study in Vietnam or any developing country to systematically examine the extent to which psychosocial factors mediate the relationship between SES and depression. The study contributes new evidence regarding the burden of depression in Vietnam. The findings have practical relevance for advocacy, for mental health promotion and health-care services, and point to the need for programs that focus on building a sense of personal mastery and self esteem. More broadly, the work presented in this Thesis contributes to the international scientific literature on the social determinants of depression.

Stress enables synaptic depression in CA1 synapses by acute and chronic morphine: Possible mechanisms for corticosterone on opiate addiction

The hippocampus, being sensitive to stress and glucocorticoids, plays significant roles in certain types of learning and memory. Therefore, the hippocampus is probably involved in the increasing drug use, drug seeking, and relapse caused by stress. We have studied the effect of stress with morphine on synaptic plasticity in the CA1 region of the hippocampus in vivo and on a delayed-escape paradigm of the Morris water maze. Our results reveal that acute stress enables long-term depression (LTD) induction by low-frequency stimulation (LFS) but acute morphine causes synaptic potentiation. Remarkably, exposure to an acute stressor reverses the effect of morphine from synaptic potentiation ( similar to 20%) to synaptic depression ( similar to 40%), precluding further LTD induction by LFS. The synaptic depression caused by stress with morphine is blocked either by the glucocorticoid receptor antagonist RU38486 or by the NMDA-receptor antagonist D-APV. Chronic morphine attenuates the ability of acute morphine to cause synaptic potentiation, and stress to enable LTD induction, but not the ability of stress in tandem with morphine to cause synaptic depression. Furthermore, corticosterone with morphine during the initial phase of drug use promotes later delayed-escape behavior, as indicated by the morphine-reinforced longer latencies to escape, leading to persistent morphine-seeking after withdrawal. These results suggest that hippocampal synaptic plasticity may play a significant role in the effects of stress or glucocorticoids on opiate addiction.

Opioid Withdrawal for 4 Days Prevents Synaptic Depression Induced by Low Dose of Morphine or Naloxone in Rat Hippocampal CA1 Area In Vivo

The formation of memory is believed to depend on experience- or activity-dependent synaptic plasticity, which is exquisitely sensitive to psychological stress since inescapable stress impairs long-term potentiation (LTP) but facilitates long-term depression (LTD). Our recent studies demonstrated that 4 days of opioid withdrawal enables maximal extents of both hippocampal LTP and drug-reinforced behavior; while elevated-platform stress enables these phenomena at 18 h of opioid withdrawal. Here, we examined the effects of low dose of morphine (0.5 mg kg(-1), i.p.) or the opioid receptor antagonist naloxone (1 mg kg(-1), i.p.) on synaptic efficacy in the hippocampal CA1 region of anesthetized rats. A form of synaptic depression was induced by low dose of morphine or naloxone in rats after 18 h but not 4 days of opioid withdrawal. This synaptic depression was dependent on both N-methyl-D-aspartate receptor and synaptic activity, similar to the hippocampal long-term depression induced by low frequency stimulation. Elevated-platform stress given 2 h before experiment prevented the synaptic depression at 18 h of opioid withdrawal; in contrast, the glucocorticoid receptor (GR) antagonist RU38486 treatment (20 mg kg(-1), s.c., twice per day for first 3 days of withdrawal), or a high dose of morphine reexposure (15 mg kg(-1), s.c., 12 h before experiment), enabled the synaptic depression on 4 days of opioid withdrawal. This temporal shift of synaptic depression by stress or GR blockade supplements our previous findings of potentially correlated temporal shifts of LTP induction and drug-reinforced behavior during opioid withdrawal. Our results therefore support the idea that stress experience during opioid withdrawal may modify hippocampal synaptic plasticity and play important roles in drug-associated memory. (C) 2009 Wiley-Liss, Inc.