1 resultado para silencing suppressors
em Instituto Politécnico de Bragança
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Aberystwyth University Repository - Reino Unido (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- Adam Mickiewicz University Repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (24)
- Andina Digital - Repositorio UASB-Digital - Universidade Andina Simón Bolívar (1)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (23)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (19)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (20)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (10)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (10)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (72)
- Brock University, Canada (9)
- CaltechTHESIS (4)
- Cambridge University Engineering Department Publications Database (12)
- CentAUR: Central Archive University of Reading - UK (19)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (22)
- Cochin University of Science & Technology (CUSAT), India (1)
- Collection Of Biostatistics Research Archive (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (4)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (36)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Archives@Colby (1)
- Digital Commons - Michigan Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (10)
- DigitalCommons@The Texas Medical Center (62)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (2)
- DRUM (Digital Repository at the University of Maryland) (5)
- Duke University (13)
- Düsseldorfer Dokumenten- und Publikationsservice (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (8)
- FUNDAJ - Fundação Joaquim Nabuco (3)
- Glasgow Theses Service (3)
- Harvard University (1)
- Helda - Digital Repository of University of Helsinki (20)
- Indian Institute of Science - Bangalore - Índia (32)
- Instituto Gulbenkian de Ciência (3)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico do Porto, Portugal (1)
- National Center for Biotechnology Information - NCBI (87)
- Nottingham eTheses (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (2)
- Portal de Revistas Científicas Complutenses - Espanha (4)
- QSpace: Queen's University - Canada (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (105)
- Queensland University of Technology - ePrints Archive (121)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (3)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (1)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (1)
- Repositorio Institucional de la Universidad de Málaga (2)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (61)
- Research Open Access Repository of the University of East London. (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (4)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- School of Medicine, Washington University, United States (2)
- Universidad de Alicante (1)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (11)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (4)
- Universidade Federal do Pará (5)
- Universidade Federal do Rio Grande do Norte (UFRN) (4)
- Universidade Metodista de São Paulo (2)
- Universita di Parma (2)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (9)
- Université de Lausanne, Switzerland (13)
- Université de Montréal (3)
- Université de Montréal, Canada (29)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (2)
- University of Queensland eSpace - Australia (19)
- University of Washington (1)
Resumo:
Ink Disease is considered one of the most important causes of the decline of chestnut orchards. The break in yield of Castanea sativa Mill is caused by two species: Phytophthora cinnamomi and Phytophthora cambivora, being the first one the foremost pathogen of ink disease in Portugal. P. cinnamomi is one of the most aggressive and widespread plant pathogen with nearly 1,000 host species. This oomycete causes enormous economic losses and it is responsible for the decline of many plant species in Europe and worldwide. Up to now no efficient treatments are available to fight these pathogens. Because of the importance of chestnut at economical and ecological levels, especially in Portugal, it becomes essential to explore the molecular mechanisms that determine the interaction between Phytophthora species and host plants through the study of proteins GIP (glucanase inhibitor protein) and NPP1 (necrosis-inducing Phytophthora protein 1) produced by P. cinnamomi during the infection. The technique of RNA interference was used to knockdown the gip gene of P. cinnamomi. Transformants obtained with the silenced gene have been used to infect C. sativa, in order to determine the effect of gene silencing on the plant phenotype. To know more about the function of GIP and NPP1 involved in the mechanism of infection, the ORF’s of gip and npp1 genes have been cloned to the pTOR-eGFP vector for a future observation of P. cinnamomi transformants with fluorescent microscopy and determination of the subcellular localization. Moreover the prediction by bioinformatics tools indicates that both GIP and NPP1 proteins are secreted. The results allow to predict the secretory destination of both GIP and NPP1 proteins and confirm RNAi as a potential alternative biological tool in the control and management of P. cinnamomi. Keywords: