Alcohol use disorders and psychiatric diseases in Colombia

Background: An accurate understanding of co-occurrence and comorbidity of alcohol use disorders (AUD) in Colombia is crucial for public health. Objective: A secondary analysis was conducted, using a 2003/2004 government´s population database to determine the lifetime associations between AUD and other mental and addictive disorders in people of Colombia aged 18-65 years. Methods: Several statistical analysis were performed: testing prevalence difference in mental disorders by whether the individual had an AUD; a stratified analysis by gender and logistic regression analyses accounting for differences in demographic, socio-economic, behavioral and self-reported health status variables. Results: People with AUD comprised 9% of the population, of which 88% were males and on average 37 years old. They were more likely to be males, be working, and be current smokers; and less likely to be at home or retired. The population with AUD had greater chance to comply with criteria for all disorders but minor depressive disorder, post-traumatic stress disorder, nicotine dependence, and oppositional defiant disorder. Conclusion: This study demonstrates a high prevalence of mental disorders in the adult population with AUD in Colombia. The findings highlight the importance of comorbidity as a sign of disease severity and impact on public health and supports the need for training of more professionals and developing appropriate interventions and services.

ALCOHOL AS A SOCIAL MEDIATOR AMONG UNIVERSITY STUDENTS

Objective: To identify the prevalence of alcohol consumption in Psychology students of a higher education institution in the city of Montes Claros, MG. Methods: Quantitative crosssectional descriptive research conducted from September to October 2014. The population consisted of 116 Psychology students from the city of Montes Claros, MG. Data were collected using the Alcohol Use Disorders Identification Test (AUDIT), the Inventário de Expectativas e Crenças Pessoais Acerca do Álcool – IECPA (Inventory of Expectations and Personal Beliefs about Alcohol), the Alcohol, Smoking and Substance Involvement Screening Test (ASSIST) and the Escala de Satisfação com o Suporte Social – ESSS (Social Support Satisfaction Scale). Descriptive analysis of data was performed using SPSS 19.0. Results: The sample had a predominance of female gender (82.75%, n=96), pardos (65.51%, n=76) and single (60.34%, n=70) individuals. Regarding the AUDIT risk classification, it was found that 49.13% (n=57) of the respondents were in the level 4, considered alcohol dependence. They reported occasional use of alcohol, smoking and other substances, which refer to ASSIST level 1 classification, with 94.82% (n=110). Regarding the IECPA, 87.06% (n=101) of the individuals were classified as level 1, with low vulnerability to the effects of alcohol. As to the ESSS, 68.10% (n=79) of the students showed high social support. Conclusion: Regarding the sample studied, it was found a high prevalence of dependence on alcohol and other legal and illegal drugs.

Anti-apoptotic effects of phyllanthin against alcoholinduced liver cell death

Purpose: To evaluate the anti-apoptotic effect of phyllanthin on alcohol-induced liver cell death in HepG2 cells alone and in co-culture with human monocytic (THP-1) differentiated macrophage cells. Methods: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were pretreated with 1, 5 and 10 μM phyllanthin for 24 h followed by 1300 mM alcohol for HepG2 cells and 2000 mM alcohol for the co-cultured cells. Thereafter, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) changes, apoptotic cell death and caspase-3/7 activities were assessed. Results: Alcohol exposure significantly increased intracellular ROS generation (p < 0.001), decreased MMP changes (p < 0.001), increased the number of apoptotic and necrotic cells (p < 0.001) and also induced higher caspase-3/7 activity (p < 0.001) in the co-culture with THP-1 differentiated macrophage cells than in HepG2 cells alone. Pretreatment of HepG2 cells and co-cultured cells with phyllanthin for 24 h prior to alcohol exposure significantly decreased intracellular production of ROS (p < 0.001) and also increased the change in MMP (p < 0.001) as well as caused a decrease in the number of apoptotic and necrotic cells (p < 0.001), but inhibited caspase-3/7 activity (p < 0.001). Conclusion: The results indicate that phyllanthin treatment may have a significant therapeutic effect on alcohol-related liver diseases.