Anti-apoptotic effects of phyllanthin against alcoholinduced liver cell death


Autoria(s): Sukketsiri, Wanida; Sawangjaroen, Kitja; Tanasawet, Supita
Cobertura

Origin of publication: Nigeria

Data(s)

07/06/2016

Resumo

Purpose: To evaluate the anti-apoptotic effect of phyllanthin on alcohol-induced liver cell death in HepG2 cells alone and in co-culture with human monocytic (THP-1) differentiated macrophage cells. Methods: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were pretreated with 1, 5 and 10 μM phyllanthin for 24 h followed by 1300 mM alcohol for HepG2 cells and 2000 mM alcohol for the co-cultured cells. Thereafter, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) changes, apoptotic cell death and caspase-3/7 activities were assessed. Results: Alcohol exposure significantly increased intracellular ROS generation (p < 0.001), decreased MMP changes (p < 0.001), increased the number of apoptotic and necrotic cells (p < 0.001) and also induced higher caspase-3/7 activity (p < 0.001) in the co-culture with THP-1 differentiated macrophage cells than in HepG2 cells alone. Pretreatment of HepG2 cells and co-cultured cells with phyllanthin for 24 h prior to alcohol exposure significantly decreased intracellular production of ROS (p < 0.001) and also increased the change in MMP (p < 0.001) as well as caused a decrease in the number of apoptotic and necrotic cells (p < 0.001), but inhibited caspase-3/7 activity (p < 0.001). Conclusion: The results indicate that phyllanthin treatment may have a significant therapeutic effect on alcohol-related liver diseases.

Formato

html

Identificador

http://www.bioline.org.br/abstract?id=pr16131

Idioma(s)

en

Publicador

Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria

Relação

http://www.tjpr.org; http://www.bioline.org.br/pr

Direitos

Copyright 2016 - Tropical Journal of Pharmaceutical Research

Fonte

Tropical Journal of Pharmaceutical Research (ISSN: 1596-5996) Vol 15 Num 5

Palavras-Chave #Hepatoprotective; Human monocytic cells; Apoptosis; Mitochondrial membrane potential; Liver diseases; Reactive oxygen species; Co-culture
Tipo

AA