Atividade antiangiogênica da forma recombinante da proteína 21 de Trypanosoma cruzi

Chagas disease, caused by the parasite Trypanosoma cruzi, is the cause of Chronic chagasic cardiomyopathy (CCC). The prospection of innovative therapeutic agents against CCC is a major task. The recombinant form of 21 (rP21), a secreted T. cruzi protein involved in host cell invasion and on progression of chronic inflammatory processes have been studied as a potential novel therapeutic target. Our present work aimed to verify and investigate the impact of rP21 in the formation of blood vessels in vitro and in vivo. First, tEnd cells were treated with different concentrations of rP21 or bacterial extract and viability and cellular adhesion were evaluated by MTT and angiogenesis inhibition by Matrigel tube formation assay and murine model. To verify the proteolytic activity of rP21 on extracellular matrix (ECM) components, fibrinogen, matrigel and fibronectin was incubated with rP21 or not. In addition, we performed proliferation assays and cell cycle analysis. Furthermore, the accumulation and distribution of F-actin was determined by Phalloidin staining using ImageJ software. Finally, tEnd cells were incubated with rP21 and the mRNA levels were analyzed by real-time PCR. Our results showed that rP21 did not alter cell viability and adhesion, but strongly inhibited vessel formation in vitro and in vivo. Tube formation assay showed that angiogenesis inhibition was dependent of the CXCR4-rP21 binding. In addition to these results, we observed that the rP21 was able to inhibit cell proliferation and promoted a significant reduction in the number of 4n cells (G2/M phase). Moreover, we found that rP21 significantly increased F-actin levels and this protein was able to modulate expression of genes related to angiogenesis and actin cytoskeleton. However, rP21 showed no significant activity on the matrix components. In this sense, we conclude that the rP21-endothelial cells (ECs) interaction via CXCR4 promotes inhibition of vessel formation through a cascade of intracellular events, such as inhibition of ECs proliferation and modulation of the expression of molecules associated with angiogenic processes and actin cytoskeleton.

Estratificação de solos em camadas horizontais utilizando evolução diferencial

This work's objective is the development of a methodology to represent an unknown soil through a stratified horizontal multilayer soil model, from which the engineer may carry out eletrical grounding projects with high precision. The methodology uses the experimental electrical apparent resistivity curve, obtained through measurements on the ground, using a 4-wire earth ground resistance tester kit, along with calculations involving the measured resistance. This curve is then compared with the theoretical electrical apparent resistivity curve, obtained through calculations over a horizontally strati ed soil, whose parameters are conjectured. This soil model parameters, such as the number of layers, in addition to the resistivity and the thickness of each layer, are optimized by Differential Evolution method, with enhanced performance through parallel computing, in order to both apparent resistivity curves get close enough, and it is possible to represent the unknown soil through the multilayer horizontal soil model fitted with optimized parameters. In order to assist the Differential Evolution method, in case of a stagnation during an arbitrary amount of generations, an optimization process unstuck methodology is proposed, to expand the search space and test new combinations, allowing the algorithm to nd a better solution and/or leave the local minima. It is further proposed an error improvement methodology, in order to smooth the error peaks between the apparent resistivity curves, by giving opportunities for other more uniform solutions to excel, in order to improve the whole algorithm precision, minimizing the maximum error. Methodologies to verify the polynomial approximation of the soil characteristic function and the theoretical apparent resistivity calculations are also proposed by including middle points among the approximated ones in the verification. Finally, a statistical evaluation prodecure is presented, in order to enable the classication of soil samples. The soil stratification methodology is used in a control group, formed by horizontally stratified soils. By using statistical inference, one may calculate the amount of soils that, within an error margin, does not follow the horizontal multilayer model.

Wam based space efficient Prolog implementation in Lisp

This thesis proposes the implementation of a space efficient Prolog implementation based on the work of David H. D. Warren and Hassan Aït-Kaci. The Common Lisp is the framework used to the construction of the Prolog system, it was chosen both to provide a space efficient environment and a rich programming language in the sense that it supply the user with abstractions and new ways of thinking. The resulting system is a new syntax to the initial language that runs on top of the SBCL Common Lisp implementation and can abstract away or exploit the underlying system.

A concepção de trabalho escolar a partir de Maurício Tragtenberg: fios e tramas de debate na (des)politização dos sujeitos sociais

This thesis, part of the research line "Work, Society and Education" analyzes, in a dialectical perspective, in the light of Tragtenberg´s studies, the conception of subject of schoolwork, considering the organization and the complexity of this context and inviting the investigative gaze. Our hypothesis is that the workers of education have, in the schoolwork, a fragile politicization field and overcoming the installed model, accepting themselves as executers with self-organizing difficulties, distanced from the effective participation, autonomy and self-management. We consider studies on the logic of the work, believing that the understanding of schoolwork is constitute into the current societal model, in whom its bureaucratic and hierarchical matrix, linked to articulated / articulators of the dominant socio-economic class interests. Scholars such as Braverman, Frigotto and Tragtenberg, among others, are important contributions to this reflection. We have analyzed the logic of the work also in dialogue with pedagogies differentiated, among them, the Libertarian defended by Tragtenberg, aiming to understand the conceptions and practices of the subjects involved. We accomplished a bibliographical critical analysis, in a dialectical character, focusing on the categories: work, schoolwork, control, autonomy and self-management, in order to understand the complexity of the praxis in study, dialoguing with the pillars categories historicity, contradiction and totality, which transversalize the developed analysis. The consideration of these concepts allows us to uncover the dominant bureaucratic structure and its possible overcoming fields. We believe contribute to problematizing and proposers studies, reflections and discussions, strengthening debates, deconstructing naturalizations and contributing to the political process of the subjects.