Effect of larval density and feeding sequence on meagre (Argyrosomus regius Asso, 1801) larval rearing

[EN] Meagre, has been proposed as a candidate for marine finfish diversification on commercial aquaculture (Quémèner, 2002, Mateos, 2007). Despite of the elevated on growing potential, the most important bottleneck of this specie is related to the limited production of fry. Larval rearing of this species, is performed mainly adapting seabream culture techniques with different success (Roo et al., 2007) However, since limited information about the optimal feeding sequences and nutritional requirements of meagre is available, more research is needed on larval rearing protocols and nutrition. Present results (elevated larval growth rate, high survival, short rotifers period) are very promising for a successful implementation at industrial scale, which helps to solve the continues lack of fry of this specie in the Mediterranean and Canary islands.

Use of different commercial Artemia sp. Enrichements in Octopus Vulgaris, paralarval rearing at initial planctonic phase: effects on growth, survival and fatty acid composition of the paralarvae

[EN] Octopus "paralarvae", are planktonic, swim actively and have high metabolic rates, requiring large quantities of live prey of adequate motility and nutritional quality ( Iglesias et al., 2000; Navarro and Villanueva, 2000, 2003). During the planktonic phase, they undergo strong morphological changes, after which the octopuses start settling to the bottom. The potential of Octopus vulgaris as candiadate for diversification of marine aquacultures are mainly due to its high food conversion rate and fast growth.( Iglesias et al 2006). Despite the research effort taken until now, paralarval rearing of O. vulgaris still suffers high mortalities which limited the industrial culture of this species. The main problems in the paralarval rearing stages are the high mortality rates and poor growth. These are attributed to the lack of standardized culture techniques and nutritional deficiencies in the diet of paralarvae, especially in n-3 highly unsaturated fatty acids (n-3 HUFA). The objective if this experience was to test different commercial live prey enrichment to improve nutritional quality of the artemia.

Effect of rearing technique over skeletal deformities incidence and osteological development in red porgy Pagrus pagrus (Linnaeus, 1758) larvae

[EN]Red porgy is a candidate species for marine aquaculture diversification.The objective of the present study was to describe the osteological development and the occurrence of skeletal deformities in Pagrus pagrus larvae in relation to the intensification of the rearing system. Fish samples were periodically collected along the development from hatching to juveniles (95days after hatching). Osteological development and the presence of skeleton abnormalities were evaluated. X-ray studies revealed a high number of fish (Semi-intensive:38.8%; Intensive:46.5 %) with skeletal deformities. No significant interaction was found on the incidence of lordosis and fused vertebrae with the rearing tecnique. However, cranial abnormalities and kyphosis incidences were significantly higher in intensive system cultured red porgy. Also, the position of fused vertebrae in this fish was located mainly in the caudal area instead of pre-hemal area for semi-intensive system reared red porgy. Present results, suggest a relationship among feeding sequence, osteological development and deformity incidence and location in red porgy larvae

Effect of rearing system intensiveness on biological features, culture performance and larval quality of meagre (Argyrosomus regius Asso, 1801) larvae

[EN]Meagre, Argyrosomus regius A., is a new species for aquaculture in south Atlantic and Mediterranean regions, that can reach a mean fresh weight of 8.02±2.51g. at 95dah. However, hatchery techniques must be improved to optimize culture performance and larval quality. Eggs of meagre were cultured under intensive (75 indv.l-1 in 2m3 tanks) and semi-intensive system (7.5 indv.l-1 in 40m3 tanks) to evaluate the effect of the intensification on biological features, stress resistance and skeletal deformities. At 30dah, despite in semi-intensive system reared larvae a higher total length (19.08± 2.3mm vs 16.00±1.54mm), dry body weight (13.09± 2.43mg vs 6.46±0.52mg), and survival after the activity test (75.0± 13.8% vs 53.3±11.5%) was found, the use of intensive systems were also very suitable and cost-effective for larval rearing of this species

Sea bream (Sparus aurata) intensive larval rearing : effects of microalgae and phages addition on the performance of the larvae

Máster Oficial en Cultivos Marinos. Trabajo presentado como requisito parcial para la obtención del Título de Máster Oficial en Cultivos Marinos, otorgado por la Universidad de Las Palmas de Gran Canaria (ULPGC), el Instituto Canario de Ciencias Marinas (ICCM), y el Centro Internacional de Altos Estudios Agronómicos Mediterráneos de Zaragoza (CIHEAM)

Identification, Abundance and Rearing of Mysids of Gran Canaria: aplication to laboratory and oceanographic respiratory metabolism studies

Programa de doctorado en Oceanografía

Effect of rearing techniques over, survival growth and skeletal abnormalities development in red porgy (Pagrus Pagrus) larvae

[EN] Red porgy is one of the most interested new species for Spanish and other Mediterranean countries. Although no industrial procedures for fry production has been yet developed. The aim of this work was to develop an industrial scale larval rearing protocols testing the viability of two different rearing techniques (semi-intensive vs intensive) in pilot scale facilities. The second objective was to obtain information about the contribution of rearing system to the apparition of morphological abnormalities such as lordosis, opercular deformities and upper/lower jaws shortening which are considered as quality descriptors in commercial marine fish fry production and seem to be related with larval culture conditions in early larval stages. For that purpose, two different larval rearing systems semi-intensive and intensive were compared using the same live feed enrichments. Biochemical composition of larvae, preys and commercial products was analysed. At 50 days post hatching six hundred fish per treatment was individually studied under stereoscope and abnormalities frequency recorded. At 95 days post hatching fry were soft X ray monitored as well. Survival and abnormalities frequency were similar between treatments although a better growth in terms of total length was obtained in the semi-intensive system.

Engorde de pulpo (Octopus vulgaris) alimentado exclusivamente con boga (Boops boops) de descarte de la acuicultura

[EN] The use of low value fish (Bogue, Boops boops), accidentally reared in seabream culture cages, as a sole food in octopus farming was tested in two different experiments located in Canary Islands. In a first experiment two different initial sizes were compared: 1,5 kg & 2,3 kg, both at a density of 11 kg/m3. Initial density was increased in a second experiments to 16 kg/m3 testing two lower initial weights: 1,0 kg & 0,8 kg. Bogue as a single feed was found to be good enough to sustain a good growth in octopus cage rearing. Best growth in terms of specific growth rate and average daily growth was obtained with the lowest grow size, 0,8 kg.

Effect of sex ratio on growth and survival in Octopus vulgaris (Cuvier, 1797) reared in floating cages

[EN]Octopus vulgaris is a potential candidate to diversify European aquaculture for its rapid growth and high market prices (Vaz Pires et al. 2004). One factor affecting industrial development of octopus culture is sexual maturation under rearing conditions. Octopus females can lose up to 30-60% of their initial body weight during egg-laying (Iglesias et al., 2000) and die after the paralarvae hatch (Guerra,1992), while a correlation between males death and spermatic sac depletion has being recently reported by Estefanell et al. (2010b). The present experiment discusses the effect of three different sex ratios on growth, sexual maturation and survival in O. Vulgaris. Conclusions: Discarded bogue from fish farms could be used as alternative diet for the final stage of O. vulgaris ongrowing ; Male segregation would maximize biomass increment ; Under the conditions described, sex ratios close to 1:1 produced higher biomass increment than 4:1

Efecto de los misidáceos en la calidad de la puesta en el caballito de mar, Hippocampus hippocampus (Linnaeus, 1758)

[EN]The importance of a suitable feeding in reproduction and spawning quality of teleost fish has been recognized as one of the major ?bottlenecks? in new aquaculture species like seahorses. Mysidacea species has been described as one of the main food for temperate seahorse species (Hippocampus hippocampus and H. guttulatus) in the wild. On the other hand, Artemia has been employed usually as marine food for rearing fish, including seahorses. The aim of this work is to study the effect of two different live preys (Artemia vs Mysis) in spawning quality of H. hippocampus broodstock. The animals were fed two times per day, six times per week. Spawning episodes and larvae quality was recorded. Seahorse fed on mysis showed significantly better results (p<0.05) than Artemia treatment, regarding spawning events, number of offspring?s and size. This fact showed the high potential of mysis as live prey for seahorses or other ornamental species.

Engorde de pulpo común Octopus vulgaris en jaulas en la comunidad Canaria [Material gráfico] [Recurso electrónico] : efecto de la dieta y la densidad inicial

Octopus vulgaris on-growing in floating cages is a promising activity implemented in Spain at industrial level, with productions of 16-32 tons/year from 1998. Nevertheless, some aspects of the culture system need to be evaluated to warrantee its profitability. In the present work absolute growth rate (AGR, g/day) and mortality (%) under two initial rearing densities, 10 and 17 kg/m3, were compared under two feeding regimes over 15 weeks. One diet was composed by bogue, supplied as ?discarded? species from local fish farms. The other diet was based on a 40-60% discarded bogue-crab Portunus pelagicus. Half of the reared octopuses were PIT-tagged and two sampling points were established along the experimental period. Regardless of dietary treatment, up to the 11th week, growth was 19 and 13 g./day for the low and high rearing density. On the other hand, up to the 11th week mortality was higher in the control diet fed group (30%), reaching 74-84% by the end of the experiment regardless of rearing density and dietary treatment, which could suggest some nutritional imbalance of the tested diets.

Evaluación de 2 sistemas de cultivo en pulpos macho Octopus vulgaris: efecto de la talla inicial y de la dieta

[EN]Octopus vulgaris on-growing in floating cages has shown promising results. Nevertheless, some aspects of the culture system need to be evaluated to warrantee its profitability. In the present work individual and group rearing, in PVC net compartments and floating cages respectively, are compared under two dietary treatments. One diet is composed by bogue, supplied as ?discarded? species from local fish farms, and the other is based on a 40-60% discarded bogue-crab Portunus pelagicus. Besides, the effect of initial size is also evaluated. All octopuses were PIT-tagged and the experiment lasted 2 months. Absolute growth rate (AGR, g./day) and mortality (%) were calculated. Control diet generated higher growth rates and lower mortality which suggests different crab requirements at higher rearing temperatures. High mortality recorded in individual rearing could be related with high initial rearing density and temperatures.

Evaluación de 2 sistemas de cultivo en pulpo común Octopus vulgaris en jaulas en la comunidad Canaria

Octopus vulgaris on-growing in floating cages is a promising activity implemented in Spain at industrial level, with productions of 16-32 tons/year from 1998. Nevertheless, some aspects of the culture system need to be evaluated to warrantee its profitability. In the present study two rearing systems and two dietary treatments were evaluated. Individual and group rearing, in PVC net compartments and floating cages respectively, were compared under two dietary treatments. One diet was composed by bogue, supplied as ?discarded? species from local fish farms, and the other was based on a 40-60% discarded bogue-crab Portunus pelagicus. All octopuses were PIT-tagged and the experiment lasted two months. Animals were sampled once throughout the experimental period and absolute growth rate (AGR, g./day) and mortality (%) were calculated. AGR of group rearing was above 30 g./day, however individual rearing showed 100% survival so biomass increment was higher. On the other hand, males grew more than females regardless of dietary treatment.

Efecto de la densidad larvaria y secuencial alimentaria en el cultivo larvario de corvina (Argyrosomus regius Asso, 1801) durante el primer mes de vida

Effect of larval density and feeding sequence on meagre (Argyrosomus regius Asso, 1801) larval rearing during the first month of life. In the present work two comparative studies of the effect of larval density and feeding sequence were performed. For such, two initial larval densities, 50 larvae.l-1 and 100 larvae.l-1 were established. In each density three feeding sequences were tested, applying different combinations of rotifers (Brachionus sp.) and Artemia sp. at different larval ages. After 30dah, standard length, dry weight and final survival were determined. Final survival was affected by initial larval density and feeding sequence. Lower density promotes better growth in dry weight and standard length for all the feeding sequences tested. In addition, feeding sequences affects larval growth and survival. Best result in survival (62,81±4,77%) were obtained with high larval density and T2 feeding sequence.