Preparative droplet counter-current chromatography for the separation of the new nor-seco-triterpene and pentacyclic triterpenoids from Qualea parviflora

The methanolic extract of the bark of the medicinal plant Qualea parviflora (Vochysiaceae) contains new nor-seco-triterpene and pentacyclic triterpenoids. They were separated in a preparative scale using droplet counter-current chromatography. The optimum solvent used was composed of a mixture of CHCl3/MeOH/H2O (43:37:20, v/v) in the descending mode and led to a successful separation of the new compound 28-nor-17, 22-seco-2 alpha, 3 beta, 19, 22, 23-pentahydroxy-Delta(12)-olecinane, besides the known triterpenoids bellericagenin B, bellericaside B and arjunglucoside I. Identification was performed by ESI-MS, H-1 NMR and C-13 NMR analyses.

Droplet deposition during spray and leaf pH in aquatic weed control

A diversidade morfológica da superfície foliar existente entre as espécies de plantas e a presença de estruturas foliares como tricomas, estômatos, cutícula e ceras podem exercer grande influência na aderência e deposição das gotas de pulverização, assim como na absorção do herbicida. Desta forma, o objetivo do presente trabalho foi estudar, em quatro espécies de plantas daninhas aquáticas (Enhydra anagallis, Eichhornia crassipes, Heteranthera reniformis e Typha subulata), a área de molhamento de gotas de pulverização nas superfícies foliares adaxial e abaxial, bem como o pH foliar. O experimento foi realizado em Botucatu-SP. As plantas foram cultivadas em caixas d'água sob condições de campo, e quando atingiram seu pleno desenvolvimento (antes do florescimento), foram realizadas as avaliações de pH foliar e da área de molhamento de gotas de pulverização. As tensões superficiais das gotas aplicadas (0,5 mL), apresentadas pelas soluções de glyphosate aplicado isolado a 5,0% v v-1 (Rodeo 480 g L-1 e.a. produto comercial), glyphosate + Aterbane BR (5,0% + 0,5% v v-1), glyphosate + Silwet L-77 (5,0% + 0,05% v v-1), além das soluções com os adjuvantes isolados, Aterbane BR (0,5% v v-1) e Silwet L-77 (0,05% v v-1) foram respectivamente 72,1; 28,7; 23,3; 37,3 e 22,1 mN m-1. As médias obtidas de pH foliar variaram entre 5,50 e 7,50, destacando-se a espécie E. anagallis com valores de 6,68 e 7,02 para as faces adaxial e abaxial, respectivamente. Dentre as plantas daninhas aquáticas avaliadas T. subulata foi a espécie que apresentou as maiores médias de área de molhamento nas faces adaxial e abaxial da folha, proporcionada pelas as soluções de glyphosate + Aterbane BR, glyphosate + Silwet L-77 e Silwet L-77, com valores de 12,99-7,03; 20,04-17,95 e 31,81-25,91 mm², respectivamente.

Droplet counter-current chromatography of indole alkaloids from Tabernaemontana hilariana

This paper reports the separation of the indole alkaloids from the benzene extract of the root barks of Tabernaemontana hilariana (Apocynaceae). The crude alkaloid fraction was fractionated by droplet counter-current chromatography using a low polarity mixture (hexane:ethyl acetate:ethanol:water). Nine indole alkaloids (3-hydroxycoronaridine, coronaridine, voacangine, 3-(2-oxopropyl) coronaridine, voacangine hydroxyindolenine, ibogamine, voacangine pseudoindoxyl, coronaridine pseudoindoxyl and tabernanthine) were identified using thin laver chromatography gas chromatography coupled with mass spectrometry and nuclear magnetic resonance spectroscopy. Copyright (C) 1999 John Wiley & Sons, Ltd.

Self-bound droplet of Bose and Fermi atoms in one dimension: Collective properties in mean-field and Tonks-Girardeau regimes

We investigate a dilute mixture of bosons and spin-polarized fermions in one dimension. With an attractive Bose-Fermi scattering length the ground state is a self-bound droplet, i.e., a Bose-Fermi bright soliton where the Bose and Fermi clouds are superimposed. We find that the quantum fluctuations stabilize the Bose-Fermi soliton such that the one-dimensional bright soliton exists for any finite attractive Bose-Fermi scattering length. We study density profile and collective excitations of the atomic bright soliton showing that they depend on the bosonic regime involved: mean-field or Tonks-Girardeau.

Colorimetric determination of sulfur dioxide in air using a droplet collector of malachite green solution

The measurement of sulfur dioxide in air at the parts-per-billion level is described. The experimental arrangement consists of two optical fibers placed on opposite sides of a liquid droplet of malachite green solution. After light has been passed through the droplet, the transmitted light is measured by a referenced photodetection arrangement. The light used in this absorption process is from a monochromatic source (lambda(max) 625 nm). This arrangement permits the variation of color in the droplet to be measured. The sulfur dioxide in the sample is collected by the droplet; it reacts with malachite green resulting in a colorless dye. The decoloration of the solution is proportional to the concentration of sulfur dioxide sampled. The signal depends on the sample flow rate. The present technique is simple, inexpensive, and permits a fast and near real time measurement while consuming very little reagent, (C) 1999 Academic Press.

Analytical chemistry in a liquid film/droplet

The measurement of nitrogen dioxide at the parts-perbillion level is described. The experimental arrangement consists of two optical fibers placed on opposite sides of and in contact with a liquid film (14-57 μL in volume) supported on a U-shaped wire guide and two tubular conduits (one of which constitutes the means for the delivery of the liquid), light from a green (555 nm) light-emitting diode enters the liquid film, composed of Griess-Saltzman reagent. The transmitted light is measured by a referenced photodetection arrangement. Sample gas flows past the droplet at a low flow rate (typically 0.10-0.25 L/min). The response is proportional to the sampling period and the analyte concentration. The limit of detection for this nonoptimized arrangement is estimated to be <10 ppb by volume for a 5 min sample. Some unusual characteristics are observed. The initial absorbance, when most of the analyte/reaction product is still near the surface, is higher than that when the content of the droplet is fully mixed. The signal depends on the sample flow rate in a nonmonotonic fashion, first increasing and then decreasing with increasing sampling rate; the specific chemistry involved in the collection and determination of NO2 may be responsible.

Separation of flavonoids and naphthopyrones from four Brazilian Paepalanthus species by droplet countercurrent chromatography

A general procedure was developed for the simultaneous separation of flavonoids and naphthopyrones from the polar extracts of the capitula from Brazilian everlasting plants is described. The ethanolic extracts of several species from the Paepalanthus genus (Eriocaulaceae) were fractionated by droplet countercurrent chromatography followed by column chromatography on pvp and sephadex LH-20. The isolated compounds were identified by spectrometric analysis and comparison with literature data. This approach led to the isolation of 9-O-β-D-glucopyranosylpaepalantine (1), 9-O-β-D-glucopyranosyl (1→6)allopyranosylpaepalantine (2), along with the flavonoids 6-methoxykaempferol (3), 3-O-β-D-glucopyranosyl-6-methoxykaempferol (4), patuletin (5), 3-Oβ-D-rutinosylpatuletin (6), 7-O-β-D-glucopyranosylquercetagetin (7), 5,7,4'-trihydroxy-6,3'-dimethoxyflavone (8) and 5,7,4'-trihydroxy-6,3'-dimethoxyflavonol (9).

Droplet formation in cold asymmetric nuclear matter in the quark-meson-coupling model

The quark-meson-coupling model is used to study droplet formation from the liquid-gas phase transition in cold asymmetric nuclear matter. The critical density and proton fraction for the phase transition are determined in the mean field approximation. Droplet properties are calculated in the Thomas-Fermi approximation. The electromagnetic field is explicitly included and its effects on droplet properties are studied. The results are compared with the ones obtained with the NL1 parametrization of the non-linear Walecka model. © 2000 Elsevier Science B.V.

Colorimetric determination of ambient ozone using indigo blue droplet

A simple and sensitive method based on a liquid droplet is described for the measurement of atmospheric ozone. A 30 μL drop of indigo blue solution is suspended in a flowing-air sampling stream. The ozone collected reacts with the indigo solution resulting in its decolorization. The colorimetric sensor is composed of two optical fibers and the source of monochromatic light was a red LED (625 nm). The calibration curve was constructed with ozone standard concentrations ranging from 37 - 123 ppbv. The detection limit achieved was 7.3 ppbv. The method considered here showed itself to be easy to apply with a fast response and a total analysis time of only 5 minutes.

Bilateral lower second molar impaction in teenagers: An emergent problem

The frequency of simultaneously impacted second and third molars in teenagers is increasing and becoming a common occurrence in adolescent oral surgery practice. The traditional treatment is the removal of the third molar by conventional access but repositioning of the surgical flap to the distal face of the first molar can predispose to complications such as pericoronitis and delayed healing of the attached gingiva. We present a case in which we use the germectomy approach to remove the impacted third molar for the eruption of the second molar through a vestibular incision. This incision offers excellent bone exposure and exit route for the third molar without disturbing the gingiva attached architecture on the distal face of the first molar providing good healing environment.

Dipolar droplet bound in a trapped Bose-Einstein condensate

We study the statics and dynamics of a dipolar Bose-Einstein condensate (BEC) droplet bound by interspecies contact interaction in a trapped nondipolar BEC. Our findings are demonstrated in terms of stability plots of a dipolar 164Dy droplet bound in a trapped nondipolar 87Rb BEC with a variable number of 164Dy atoms and interspecies scattering length. A trapped nondipolar BEC of a fixed number of atoms can bind only a dipolar droplet containing fewer atoms than a critical number for the interspecies scattering length between two critical values. The shape and size (statics) as well as the small breathing oscillation (dynamics) of the dipolar BEC droplet are studied using numerical and variational solutions of a mean-field model. We also suggest an experimental procedure for achieving such a 164Dy droplet by relaxing the trap on the 164Dy BEC in a trapped binary 87Rb-164Dy mixture. © 2013 American Physical Society.

Surfactant-cosurfactant interactions and process parameters involved in the formulation of stable and small droplet-sized benznidazole-loaded soybean O/W emulsions

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Distribución volumétrica, simetría del chorro y diámetro de gotas de las boquillas TF-VS2

El conocimiento de las características de las diferentes boquillas de pulverización tiene una importancia imprescindible para la adecuada y correcta recomendación de su uso. El objetivo de este trabajo fue determinar el espaciamiento máximo en la barra horizontal de pulverización, la simetría del chorro y el diámetro de gotas en boquillas de pulverización TF-VS2. El trabajo fue realizado en mesa de deposición, en donde fueron utilizadas las presiones de 100, 200 y 300 kPa en las alturas de trabajo de 40 y 50 cm. La simetría del chorro fue determinada en función de dos metodologías (empírica y trigonométrica). También se determinó el tamaño de las gotas, utilizando el método de difracción de rayos laser, en función de dos caldos de pulverización constituidos por agua y por agua con adyuvante en las tres presiones de trabajo ya descritas. Los espaciamientos máximos entre las boquillas en la barra de pulverización no pueden rebasar los 70 y 82 cm, admitiéndose el CV de un 10% para las alturas de 40 y de 50 cm respectivamente. La mayor presión proporcionó el menor diámetro mediano volumétrico (DMV) y la peor uniformidad de gotas, además del mayor porcentaje de gotas susceptibles a deriva, así como también aumentó la simetría entre los chorros.

Distribuição volumétrica e diâmetro de gotas de pontas de pulverização de energia hidráulica para controle de corda-de-viola

As pontas de pulverização são responsáveis pela formação das gotas, e cada ponta apresenta características próprias relacionadas ao espectro de gotas e perfil de deposição, específicas para determinados alvos. Este trabalho teve o objetivo de caracterizar o diâmetro e a uniformidade das gotas e o perfil de distribuição volumétrica das pontas de pulverização AI 110015 e TTI 110015, bem como seu efeito sobre a mortalidade de corda-de-viola, com herbicida pré-emergente, associado ou não a adjuvantes. Avaliou-se o número de plantas emergidas e os pesos secos da parte aérea e radicular das plantas. Os perfis de distribuição volumétrica para a altura de 40 cm foram avaliados em mesa de deposição. A partir dos perfis de distribuição, simulou-se o padrão de deposição ao longo da barra de pulverização. O espectro do diâmetro de gotas foi determinado em analisador de tamanho de partículas por difração de luz laser . O herbicida diuron + hexazinona foi eficiente no controle em préemergência de corda-de-viola, podendo ser utilizado polimetil siloxano organomodificado ou óleo mineral como adjuvantes, associados às pontas de pulverização AI 110015 ou TTI 110015. O uso de adjuvantes proporcionou aumento no diâmetro mediano volumétrico e redução na porcentagem de gotas com diâmetro inferior a 100 µm. O espaçamento sugerido entre os bicos na barra de pulverização foi de 70 cm para o modelo AI 110015 e 80 cm para o modelo TTI 110015.