7 resultados para RECOMBINANTS
em Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho"
Selection of the best source of carbon for production of recombinants enzymes in liquid fermentation
Resumo:
Heterokaryons were obtained by hyphal anastomosis in Trichoderma pseudokoningii using strains with morphologic and double auxotrophic genetic markers. Stable haploid and unstable hyperhaploid recombinants were detected in monosporic colonies derived from heterokaryons and selected in appropriate media or by an enrichment filtration technique. Diploids were not observed and the recombinant frequencies were higher than those found in fungi with a typical parasexual cycle. The mechanism of recombinant emergence may involve a transient diploid formation followed by chromosomal losses at the hyphal stages, resulting in haploid and hyperhaploid recombinants. The possibility of intrachromosomal rearrangement with the involvement of cytoplasmatic DNA as a heterokaryotic transformation system cannot be excluded.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Pós-graduação em Genética - IBILCE
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Pós-graduação em Ciências Farmacêuticas - FCFAR
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)