Indicator-free electrochemical DNA hybridization biosensor

A new electrochemical hybridization biosensor protocol without an external indicator is described. The biosensor format involves the immobilization of inosine-substituted (guanine-free) probe onto the carbon paste transducer, and a direct chronopotentiometric detection of the duplex formation by the appearance of the guanine oxidation peak of the target. Such a use of the intrinsic DNA electrochemical response for monitoring hybridization events offers several advantages (over the common use of external indicators), including the appearance of a new peak, a Aat background, or simplicity. A 4 min short hybridization period allows a detection limit around 120 ng/ml. Performance characteristics of the sensor are described along with future prospects. (C) 1998 Elsevier B.V. B.V. All rights reserved.

The amphibians of São Paulo State, Brazil amphibians of São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aortic aneurysm surgery: problems and innovations

Um longo caminho foi percorrido desde as primeiras intervenções cirúrgicas feitas para o tratamento do aneurisma de aorta abdominal. Ao longo deste tempo, várias inovações foram criadas, no sentido de reduzir a invasividade dos procedimentos e melhorar sua segurança e durabilidade. Nesta revisão, são discutidos os principais e recentes avanços em intervenções sobre aneurismas aórticos, incluindo a restauração aórtica endovascular, a cirurgia aórtica videolaparoscópica, as técnicas híbridas convencionais e endovasculares, as técnicas combinadas videolaparoscópicas e endovasculares, bem como as perspectivas futuras, tanto para aorta torácica como abdominal. Diante de tantas transformações e evolução, o cirurgião vascular moderno terá que ter sua mente aberta para as novidades e desenvolver capacitação ampla com diferentes técnicas para proporcionar a melhor opção terapêutica para seus pacientes.

Otimização de controladores robustos de sistemas dinâmicos sujeitos a falhas estruturais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Percepção e perspectivas dos adolescentes sobre a TV digital

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Para além dos Contos de Fadas: o ideal e o real no pensamento das mulheres sobre o casamento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Explanations and Representations of Violence by School-age Brazilian Youths

The violence staged by young people has often been subjected to scientific analysis. The way youths speak, in their role as aggressors or as victims, is examined to determine how they experience violence in a number of different spheres. Repeated group interviews are used to analyze how violence is explained and depicted within the family, at school and in the neighbourhood by two groups of young people (14-17 years old) attending the same school on the outskirts of Rio Claro, Sao Paulo, Brazil. One of the groups involved is identified by the school as violent, and the other, as non-violent. Discourse analysis leads to two conclusions. First, the different contexts of violence infuse a mistrust of institutions, the environment and personal relationships into the subjects' experience, forming a fabric that clouds future prospects. Second, the group of youths identified as violent have a more simplistic, pessimistic view of reality: They see the world in black and white, and they lay no stock in the possibility that violence can be avoided. Consequently, they use violence and understand violence as a defensive strategy that gives one identity. On the other hand, the group identified as nonviolent feels it possible to intervene in situations with nonviolent tools like words. For the young subjects, violence is a context that they assume; it cancels their ability to identify rules and institutions, but it does not generate an effective interaction strategy. Violence causes their social microcontext (in which action prevails over meaning or meaning equals action) to assume overblown dimensions. Any intervention strategy must take into account this indissoluble unity between meaning and action.

Brazilian genetic database of chromosome X

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aproveitamento de resíduos na composição de painel sarrafeado amarrado: um estudo econômico

The manufacture of plywood panel already has about eighty years in Brazil. In general, plywood panels differ by their nature, composition and manufacturing; the most common MDF, OSB, particle and the joist. Some of these products can be generated fromindustrial waste, as the panelblockboard, you can use in their training waste destop boards (Battens), from industrial processes or processing of primary wood. This work aims to present the detailed study of the economic viability of utilization of industrial solid waste timber through the acquisition of a machine amarradeira of joists in a wood processing unit in city Itapeva-SP. We studied the application of wastein the composition of kernels blockboard panel tied its commercial acceptance and future prospects market. We noticed great economic advantage in investing in the study, compared with the transformation process of the joists in biomass energy, although the investment decision involves not only financial matters but also the acceptance market the product to be manufactured

Relações Públicas nas agências de comunicação: uma análise dos discursos dos mercados

Public Relations is an area of communication that has many definitions given by both professional associations and by many authors. Academic and professional discussions on a precise definition of the area are recurrent. Considering this issue, it was decided to study the relationship between public relations and communications agencies, assuming that this is a market segment that includes the public relations. This paper analyzes the discourses of communication agencies and how the Public Relations are handled by them. For this, it was used theories about the history of public relations as well as definitions and concepts of its main authors. Furthermore, we present the characteristics of communication agencies, the contexts associated with its emergence in Brazil and the future prospects of this market segment in the opinion of the professionals who work in it. The study about the discourses of communications agencies was based on French Discourse Analysis, using books from important authors of the area, such as José Fiorin and Helna Brandão. The discourses analyzed were those present on websites of three communications agencies in the state of Sao Paulo. It was noted that not always the participation of public relations in communication agencies is highlighted, although the services of these agencies are based on techniques and typical functions of Public Relations. Above all, what the discourses are seeking to highlight are the experience and expertise in organizational communication owned by its staff

Uma revisão sobre o uso de medicamentos naturais para tratamento de tumores

This study objective was to review a selection of publications on the treatment of benign and malignant tumors with the use of natural medicines obtained from terrestrial and marine flora, both for use in chemotherapy and photodynamic therapy. There are many medications already being used for this purpose and many are still being tested in vivo and in vitro, but all with great future prospects for inclusion in the market

Dendrimers as potential platform in nanotechnology-based drug delivery systems

The dendrimers of poly (amidoamine) (PAMAM) are nanoparticles which have proven succeed in transporting drugs due to high solubility, low toxicity and ability to control drugs release. Studies have explored the biological potential of dendrimers such as to transport genes, development of vaccines, antiviral, antibacterial and anticancer therapies. This review of literature on the PAMAM dendrimers discusses the architecture and general construction of dendrimers and intrinsic properties of the PAMAM. This study also describes how the PAMAM interact with many drugs and the potential of these macromolecules as well as drug nanocarriers in transdermal routes of administration, ocular, respiratory, oral and intravenous administration. Dendrimers promises good future prospects for the biomedicine.

Conflito de interesses ambientais: o passado, o presente e o futuro da gestão sustentávelno município de Botucatu (SP)

Entrepreneurs responsible for the housing project “Vale do Sol III”, (claim of deployment), proposed a technique, in order to resolve conflicts of interest arising in respect Vale do Sol I residents (already implemented). Conflicts taken place in accordance with environmental problems that originated (past) in poor drainage of rainwater from Água Fria stream. This work, based on study case method with deductive approach, analyzed the environmental aspects of the area in order to contribute to the process of sustainable management of medium-sized cities (present), as well as analyzing the prospects for adequate outcome environmental demand (future) . In terms of technical and methodological procedures, was used geoprocessing softwares for the purpose of supporting analysis. It was concluded that, technique proposed at Vale do Sol III enterprise, don’t have necessary, sufficient and satisfactory solution in order to, at least, mitigate the problems of area sustainability. In terms of future prospects, it was concluded that, good sustainable environmental management, with preventive measures, from the point of view of mitigating the environmental and urban conflicts, and corrective measures.

O ensino de sistemática e taxonomia biológica no ensino médio da rede estadual no município de São Carlos - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

As temporalidades que caracterizam a agricultura familiar do município de Marmeleiro (PR)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)