Indicator-free electrochemical DNA hybridization biosensor
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
30/11/1998
|
Resumo |
A new electrochemical hybridization biosensor protocol without an external indicator is described. The biosensor format involves the immobilization of inosine-substituted (guanine-free) probe onto the carbon paste transducer, and a direct chronopotentiometric detection of the duplex formation by the appearance of the guanine oxidation peak of the target. Such a use of the intrinsic DNA electrochemical response for monitoring hybridization events offers several advantages (over the common use of external indicators), including the appearance of a new peak, a Aat background, or simplicity. A 4 min short hybridization period allows a detection limit around 120 ng/ml. Performance characteristics of the sensor are described along with future prospects. (C) 1998 Elsevier B.V. B.V. All rights reserved. |
Formato |
197-203 |
Identificador |
http://dx.doi.org/10.1016/S0003-2670(98)00503-0 Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 375, n. 3, p. 197-203, 1998. 0003-2670 http://hdl.handle.net/11449/8701 10.1016/S0003-2670(98)00503-0 WOS:000077069300001 |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
Analytica Chimica Acta |
Direitos |
closedAccess |
Palavras-Chave | #DNA hybridization #biosensors #guanine #inosine #carbon paste electrodes |
Tipo |
info:eu-repo/semantics/article |