Manejo mecânico e cultura de cobertura na entrelinha da seringueira (PB 235 e RRIM 701) e os atributos físicos de um latossolo vermelho no Planalto Paulista

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização mecânica e térmica da borracha natural formulada e vulcanizada dos clones: GT 1, IAN 873, PB 235 e RRIM 600

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra: A solvent gate for the active site of pH-sensitive luciferases

Beetle luciferases emit a wide range of bioluminescence colors, ranging from green to red. Firefly luciferases can shift the spectrum to red in response to pH and temperature changes, whereas click beetle and railroadworm luciferases do not. Despite many studies on firefly luciferases, the origin of pH-sensitivity is far from being understood. Through comparative site-directed mutagenesis and modeling studies, using the pH-sensitive luciferases (Macrolampis and Cratomorphus distinctus fireflies) and the pH-insensitive luciferases (Pyrearinus termitilluminans, Phrixotrix viviani and Phrixotrix hirtus) cloned by our group, here we show that substitutions dramatically affecting bioluminescence colors in both groups of luciferases are clustered in the loop between residues 223-235 (Photinus pyralis sequence). The substitutions at positions 227, 228 and 229 (P. pyralis sequence) cause dramatic redshift and temporal shift in both groups of luciferases, indicating their involvement in labile interactions. Modeling studies showed that the residues Y227 and N229 are buried in the protein core, fixing the loop to other structural elements participating at the bottom of the luciferin binding site. Changes in pH and temperature (in firefly luciferases), as well as point mutations in this loop, may disrupt the interactions of these structural elements exposing the active site and modulating bioluminescence colors. © 2007 The Authors.

Effect of supplementation frequency on intake, behavior and performance in beef steers grazing Marandu grass

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise energética do novo sistema de produção de maracujá amarelo na região de Marília-SP

The study aimed to evaluate energy indicators of a new production system of yellow passion fruit in Marilia-SP. Analyzed the inputs of the biological energy, fossil and industrial and output energy in the form of fruits produced per unit area as well as five energy indicators. The input energy amounted 155,810.13MJ ha(-1). Chemical fertilizers and pesticides accounted for 71% and 24% of indirect energy industry, respectively. The activity generated per cycle / ha, 587.700.00MJ, and cultural productivity 0.19MJ kg(-1). The efficiency culture was 3.77. The cultural energy net totaled 431,889.87MJ ha(-1). The energy efficiency (4.17) and energy balance (550,312.91MJ ha(-1)) was favorable. It was concluded that the direct energy consumption from fossil fuels, even though significant, yet permits the production of the fruit of an environmentally sustainable manner. Chemical fertilizers and pesticides were the most representative of indirect energy industry due to the intensification of fertilization as a means of disease prevention.

Jornal da UNESP, 2015, ano XXXI, n. 312

Jornal elaborado pela Assessoria de Comunicação e Imprensa da Reitoria da UNESP