147 resultados para Transposable elements


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Augmented Reality (AR) systems which use optical tracking with fiducial marker for registration have had an important role in popularizing this technology, since only a personal computer with a conventional webcam is required. However, in most these applications, the virtual elements are shown only in the foreground a real element does not occlude a virtual one. The method presented enables AR environments based on fiducial markers to support mutual occlusion between a real element and many virtual ones, according to the elements position (depth) in the environment. © 2012 IEEE.

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This work studied the influence of the rare earth (Ce3+ and Ce4+) elements concentration in polysiloxane flints deposited on copper by dip-coating process, and evaluated their resistance in a 3.5 wt.% NaCl medium. Classical electrochemistry techniques were used as open circuit potential, polarization curves and electrochemical impedance spectroscopy. The results revealed that by adding low concentration of Ce4+ ions, the coating prevents the electrolyte uptake any longer retarding the substrate degradation consequently. ©The Electrochemical Society.

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Fish belonging to the genus Hypostomus are known for exhibiting a striking diversity in its karyotype structure, however the knowledge concerning the distribution patterns of heterochromatin and location of repetitive DNA sequences in the karyotypes is still limited. Aiming a better understanding of the chromosomal organization in this group, we analyzed three sympatric species of Hypostomus collected in the Hortelã stream, a component of the Paranapanema River basin, Botucatu/SP/Brazil. The analyses involved the cytogenetic characterization and chromosomal mapping of repetitive sequences and intra/interspecific comparisons using sequences of the cytochrome C oxidase subunit I. The results revealed that H. ancistroides presents a karyotype with 2n = 68 chromosomes, H. strigaticeps 2n = 72 chromosomes, and H. nigromaculatus 2n = 76 chromosomes. In addition to differences found in the diploid number, it was also observed variations in karyotypic formulae, amount of constitutive heterochromatin, and location of nucleolus organizer regions. The cytogenetic mapping of 5S and 18S rDNA, as well as of the H3 histone gene, disclosed a differential dispersion process among the three species. In some cases the Rex1 transposable element showed to be co-located with 5S rDNA sites. The molecular analyses support the cytogenetic data and represent an additional tool for the characterization of the analyzed species. The results evidenced that chromosomal variations are not restricted to differences in diploid number or karyotypic macrostructure in the genus Hypostomus, indicating that events such as transposition of heterochromatin and rDNA segments may participate in the differentiation process occurred in these species. © 2013 Springer Science+Business Media Dordrecht.

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O experimento foi realizado na área experimental do Departamento de Engenharia Rural da Faculdade de Ciências Agronômicas – Unesp/Botucatu, Estado de São Paulo, em duas estufas dispostas em diferentes orientações geográficas, Leste/Oeste e Norte/Sul. A alface (Lactuva sativa L.) cv. Elisa foi cultivada em ambas estufas, sendo semeada em 05/05/99, transplantada em 29/05/99 e colhida em 31/06/99. Utilizou-se tensiômetros para monitorar o potencial de água no solo para realizar o manejo do sistema de irrigação por gotejamento. Microevaporímetros eqüidistantes de 3 m e colocados em 3 alturas, 0.50, 1.00 e 1.50 m, termohigrógrafos e tanques Classe “A” foram instalados nas duas estufas. Através de análise geoestatística, não se observou dependência espacial nem variabilidade espacial da evaporação nas duas estufas. Entretanto, a altura dos evaporímetros apresentou diferenças significativas: a evaporação à altura de 1.50 foi menor que nas outras duas.As médias de temperatura, umidade relativa e déficit de pressão de vapor do arnão diferiram estatisticamente entre as estufas e o ambiente externo. Os valores médios de evaporação de água no tanque Classe A instalado fora das estufas diferiram estatisticamente quando comparados com os instalados no interior das estufas, porém, entre as orientações não se constatou diferença significativa. Pôde-se verificar que não houve diferença significativa das características agronômicas da alface em ambas orientações estudadas. No entanto, houve diferença significativa para essas características entre os canteiros no interior das mesmas, havendo variância espacial para os dados de matéria fresca apenas na estufa N/S.

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In this paper we present a set of generic results on Hamiltonian non-linear dynamics. We show the necessary conditions for a Hamiltonian system to present a non-twist scenario and from that we introduce the isochronous resonances. The generality of these resonances is shown from the Hamiltonian given by the Birkhof-Gustavson normal form, which can be considered a toy model, and from an optic system governed by the non-linear map of the annular billiard. We also define a special kind of transport barrier called robust torus. The meanders and shearless curves are also presented and we show the most robust shearless barrier associated with the rotation numbers.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Some changes in the application of the numeric trapezoidal integration are analyzed for applications considering pi circuits. It is considered numeric and computational proceedings for improving the numeric results obtained with associations of pi circuits. In numeric integration solutions of the linear systems, it is common to represent these associations of pi circuits by only one matrix. This representation introduces undesirable numeric oscillations in simulations of the dynamics of wave propagation in electrical systems. The proposed changes improve the results of application of cascades of pi circuits associated to the trapezoidal integration, avoiding that the numerical oscillations, or Gibb's oscillations, have high values and are slowly damped. For the carried out simulations, different number of pi circuits and voltage sources are checked, confirming the reduction of the influence of the numeric oscillations on the obtained results. (C) 2014 Elsevier B.V. All rights reserved.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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This paper presents a new technique to model interfaces by means of degenerated solid finite elements, i.e., elements with a very high aspect ratio, with the smallest dimension corresponding to the thickness of the interfaces. It is shown that, as the aspect ratio increases, the element strains also increase, approaching the kinematics of the strong discontinuity. A tensile damage constitutive relation between strains and stresses is proposed to describe the nonlinear behavior of the interfaces associated with crack opening. To represent crack propagation, couples of triangular interface elements are introduced in between all regular (bulk) elements of the original mesh. With this technique the analyses can be performed integrally in the context of the continuum mechanics and complex crack patterns involving multiple cracks can be simulated without the need of tracking algorithms. Numerical tests are performed to show the applicability of the proposed technique, studding also aspects related to mesh objectivity.

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This work tries to outline the theoretical background established by archival science manuals, based mainly on discourse analysis as a key discipline to understand which the differences are and points of conceptual commonality in archival science traditions studied that to some extent, are additional. As archival science is a product of historically constructed knowledge, the context of conceptual production ultimately reflects in the different schools of thought and in the methodological approach. The manuals discussed here were selected by their canon and their wide dissemination in the archival science field, and are: the Manual for the arrangement and description of archives of the Association of Dutch Archivists (1898), and Jenkinson's A manual of archive administration including the problems of war archives and archive making. We considered how in these manuals two key concepts are discussed as for organization methodology in archival science: description and classification, establishing, from these concepts, which are the intertwined discourses in these texts and relating them to their historical aspects.

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In this paper, natural frequencies were analyzed (axial, torsional and flexural) and frequency response of a vertical rotor with a hard disk at the edge through the classical modal and complex analysis. The equation that rules the movement was obtained through the Lagrangian formulation. The model considered the effects of bending, torsion and axial deformation of the shaft, besides the gravitational and gyroscopic effects. The finite element method was used to discretize the structure into hollow cylindrical elements with 12 degrees of freedom. Mass, stiffness and gyroscopic matrices were explained consistently. The classical modal analysis, usually applied to stationary structures, does not consider an important characteristic of rotating machinery which are the methods of forward and backward whirl. Initially, through the traditional modal analysis, axial and torsional natural frequencies were obtained in a static shaft, since they do not suffer the influence of gyroscopic effects. Later research was performed by complex modal analysis. This type of tool, based on the use of complex coordinates to describe the dynamic behavior of rotating shaft, allows the decomposition of the system in two submodes, backward and forward. Thus, it is possible to clearly visualize that the orbit and direction of the precessional motion around the line of the rotating shaft is not deformed. A finite element program was developed using MATLAB (TM) and numerical simulations were performed to validate this model. Natural frequencies and directional frequency forced response (dFRF) were obtained using the complex modal analysis for a simple vertical rotor and also for a typical drill string used in the construction of oil wells.

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Cancer is a multistep process that begins with the transformation of normal epithelial cells and continues with tumor growth, stromal invasion and metastasis. The remodeling of the peritumoral environment is decisive for the onset of tumor invasiveness. This event is dependent on epithelial–stromal interactions, degradation of extracellular matrix components and reorganization of fibrillar components. Our research group has studied in a new proposed rodent model the participation of cellular and molecular components in the prostate microenvironment that contributes to cancer progression. Our group adopted the gerbil Meriones unguiculatus as an alternative experimental model for prostate cancer study. This model has presented significant responses to hormonal treatments and to development of spontaneous and induced neoplasias. The data obtained indicate reorganization of type I collagen fibers and reticular fibers, synthesis of new components such as tenascin and proteoglycans, degradation of basement membrane components and elastic fibers and increased expression of metalloproteinases. Fibroblasts that border the region, apparently participate in the stromal reaction. The roles of each of these events, as well as some signaling molecules, participants of neoplastic progression and factors that promote genetic reprogramming during epithelial–stromal transition are also discussed.