Diagnóstico sorológico e molecular de agentes transmitidos por artrópodes em aves carnívoras

Pós-graduação em Medicina Veterinária - FCAV

Comparação entre métodos diagnósticos da tuberculose em bovinos abatidos em matadouros-frigoríficos do Estado de São Paulo

Pós-graduação em Medicina Veterinária - FCAV

Avaliação da pressão arterial sistêmica em cães obesos: comparação entre os métodos oscilométrico e doppler ultrassônico

Obesity is the most common nutritional problem in dogs and it can cause various harmful effects on animal health. However, the effect of this condition on systolic blood pressure (SBP) in obese dogs is controversial. The indirect method of measuring PAS is the most commonly used in veterinary medicine for the diagnosis of systemic hypertension, because it is more practical and easy to access. There is little scientific information about the comparison of the two non- invasive methods of measurement of blood pressure in obese dogs. Therefore, the objective of this study was to evaluate the SBP in obese dogs by comparing both indirect methods of measuring blood pressure, with oscillometric and doppler vascular in order to verify the differences in blood pressure values, but also the best method to assess the SBP dogs in this body score condition. The study complain blood pressure measurement of 50 dogs, with were divided in obese dogs with mean body condition score (BCS) of 8.42 +/- 0.50 (n = 25) and dogs with ideal BCS of 4.56 +/- 0.51 (n = 25). At comparison of blood pressure values, SBP values obtained by doppler method (152 +/- 16mmHg) were higher than the oscillometric (136 +/- 11mmHg). Correspondence analysis determined by multivariate statistical analysis showed correlation between body condition and the procedure of the SBP. These findings suggest that the indirect method doppler can better reflect the SBP in obese dogs.

Influência do método de avaliação no diagnóstico da dor muscular de início tardio após corrida em declive

Several methods are used towards delayed onset muscle soreness appraisal. This study's goal was to investigate, amongst three commonly adopted methods, which one would be the most effective (the one that shows higher values) in the quantification of this phenomenon. 10 male subjects, of age 22.8 ± 3.1 years old, weight 80 ± 12.4 kg, height 174 ± 0.07 cm, without recent experience with strength training (lower limbs) and/or running took place in this study. All subjects carried out a muscle damage induction protocol, which consisted of 30 minute downhill running (-16% or -9.09º) at 80% speed compared to their maximum oxygen consumption. Muscle damage determinants such as isometric peak torque, knee joint range of motion and circumference of the medial portion of the thight were measured before, during, 24, 48, 72 and 96 hours after downhill running. The subjective pain perception was measured simultaneously with the other determinants through three different tests: sitting on and getting up of a chair; climbing and descending from a 45cm step; and self thigh palpation. After going through all subjective pain perception tests, the subjects filled out a visual analog scale with their perception of pain. Muscle damage changes over time were compared through variance analysis (ANOVA) one way for repeated measures. Subjective pain perception values obtained in all three different tests were compared through two way ANOVAs for repeated numbers. The significance level adopted in this study was z ≤ 0.05. The results showed that the step test was the on which better evaluated the delayed onset muscle soreness. No significant differences were found through the ADM and CIR recovery markers. Downhil running determined reduced of PTI (~22.4%). Significant links between pain were obtained for both subjective pain perception tests starting at 24 and 48 hours, where the highest registered average happened in the step test after 48h, with high...

Diagnóstico ambiental em área com vazamento de combustíveis por meio da integração de dados geofísicos e geoquímicos no município de São Manuel - SP

The contamination of the physical environment occurs as a result of the lack of planning and inadequate management of hazardous products. Due to the difficulty of the characterization and study of soil and groundwater, the contamination has been long neglected. The characterization of these areas by research techniques through direct methods such as physical and chemical analysis, are widely used in the diagnosis and monitoring. However, despite presenting quantitative results, this method is not representative in spatial terms. The purpose of this work is to bring geophysical complementation with the diagnosis, bringing the spatial element for the detection of the contamination plume. In the study area, there was a railway accident in 1999 in which three wagons fell and spilled 240,000 L of diesel onto the soil. The main objective is to assess the presence of contaminants in the residual phase and dissolved in the subsurface, using the technique of Electrical Tomography (ETR), understand the effects of natural attenuation process in the degradation of hydrocarbons and changes in electrical properties in soil and groundwater. The results allowed the definition of three distinct patterns of resistivity region with presence of dissolved phase, set in a historical chemical analyzes: low values (> 60Ω.m) associated with the presence of hydrocarbons in an advanced stage of degradation, intermediate values (260Ω.me 511Ω .m) indicative of coexistence of residual hydrocarbons and dissolved phase, and high values (> 1000Ω.m), which reveal the prevalence of residual phase with incipient degradation, possibly in high toxicity concentrations for effective action of biodegradation processes. The diagnostic of areas with potential accumulation of residual phase can subsidize the planning of remediation techniques and promote the complete decontamination of the area

Positividade do imunomarcador FOXP3 em células T reguladoras em lesões de micose fungoide em fases clínicas iniciais e em dermatoses inflamatórias como fator auxiliar para o diagnóstico diferencial

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Perfil epidemiológico dos participantes do Programa de diagnóstico e prevenção do câncer de boca em Mato Grosso

To determine the profile of the patients who participated in the program of oral cancer diagnosis and prevention in Mato Grosso, from 2008 to 2009. Materials and Methods: The medical records of the treated patients were examined. Results: 1.293 records were analyzed, with a predominance of female patients (60%) and a greater participation of patients from 10 to 19 years of age (20%). Reports of pre-existing illnesses amounted to 302 recorded cases. In addition, 292 variations of normality could be also identified. During the period, 25 participants were recommended for exfoliative cytology and 133 for biopsy, as they presented oral lesions that raised the suspicion of malignancy. Conclusions: Campaigns to raise awareness of and prevent oral cancer are very important and should be extended to the entire population of the state of Mato Grosso, either by means of the local University Center (UNIVAG), or by other agencies and health institutions.

Importância da avaliação hematológica e sorológica (dot-blot ELISA) no diagnóstico de erliquiose em cães

This study compared the hematological and serological analysis of diagnosis of canine ehrlichiosis. The survey of Ehrlichia canis was performed through the evaluation of blood smears from 150 dogs. The serological test was performed on 12 samples selected by the platelet count (less than 170,000 platelets / uL). Serologic testing was performed with the Imunocomb kit - Dot-blot-ELISA. No cytoplasmatic inclusion characteristic of morula of E. canis was found in blood smears. In serologic testing, eight samples were positive for Ehrlichia canis, concluding that thrombocytopenia is an important hematological finding of ehrlichiosis diagnosis and the detection of Ehrlichia canis morulae is uncommon. The serological evaluation Dot-blot ELISA is an accurate and brief diagnosis method of canine ehrlichiosis, been the most appropriate to be used in veterinary practice routine.

Displasia neuronal intestinal: análise de critérios morfológicos e comparação de métodos diagnósticos

Pós-graduação em Patologia - FMB

Diagnóstico laboratorial de Mycobacterium spp. em Botucatu e região, utilizando a técnica Reação em Cadeia da Polimerase (PCR) em material biológico e avaliação de condições associadas

Pós-graduação em Saúde Coletiva - FMB

Comparação de métodos para extração de dna na reação em cadeia da polimerase para detecção de Salmonella enterica sorovar enteritidis em produtos avícolas

The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.

Características do processo de diagnóstico de hanseníase no atendimento primário e secundário

Contexto e objetivo: A hanseníase caracteriza-se por ter diagnóstico eminentemente clínico, principalmente através da constatação de hipo ou anestesia das lesões, ou seja, sem necessariamente o uso de exames adicionais, como baciloscopia e biópsia, utilizados para classificação clínica da doença. Buscou-se definir se há tendência à desvalorização da avaliação clínica em favor do excesso de exames complementares, investigar a adequação do registro nos atendimentos da atenção básica e compará-los com o obtido em serviço de média complexidade. Desenho e local: Estudo transversal, de base populacional, utilizando registros de prontuários, realizado em dois âmbitos de atendimentos de saúde da cidade de Campinas (SP): os Centros de Saúde e a assistência dermatológica especializada de hospital de ensino. Métodos: Os registros foram investigados por meio da aplicação de checklist, contendo itens indispensáveis ao atendimento a hansenianos. Associações foram testadas pela prova de Goodman e ao nível de 5% de significância. Resultados: Foram avaliados 33 prontuários oriundos dos centros de saúde e 36 do hospital, observou-se polarização entre registros adequados e ausentes, evidenciando a presença de efeito tudo ou nada nos atendimentos; de 26 variáveis, apenas em 5 (19,23%) obteve-se predomínio da avaliação do hospital sobre os centros de saúde; evidenciou-se dependência de exames complementares, solicitados em 67 dos 69 atendimentos (97,10%), para diagnóstico da doença. Conclusão: Confirmou-se, neste estudo, déficit da avaliação médica e indicação inadequada de exames adicionais. Por associar-se com excesso de solicitação de exames, a observação clínica mostrou-se incompleta, comprometendo a capacidade diagnóstica dos atendimentos a hansenianos.

Prevalência de bexiga hiperativa e avaliação do impacto desse diagnóstico em mulheres de diferentes faixas etárias

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ensaio clínico randomizado duplo-cego do perfil inflamatório e do nível de estresse oxidativo em indivíduos com diagnóstico polissonográfico de SAOS antes e após o uso de CPAP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)