Microencapsulação por gelificação iônica e interação eletrostática do corante de buriti (Mauritia flexuosa L. f.)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Removal of glyphosate herbicide from water using biopolymer membranes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Marine-derived fungi: diversity of enzymes and biotechnological applications

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of mucoadhesive polymers on the in vitro performance of insulin-loaded silica nanoparticles: Interactions with mucin and biomembrane models

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Combining xanthan and chitosan membranes to multipotent mesenchymal stromal cells as bioactive dressings for dermo-epidermal wounds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bacterial cellulose biocomposites for guided tissue regeneration

Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of chondroitin sulfate and hyaluronic acid (1% w/w) to the culture medium before the bacteria is inoculated. Besides, biomimetic precipitation of calcium phosphate of biological interest from simulated body fluid on bacterial cellulose was studied. Chondroitin sulfate and hyaluronic acid influences in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR), XRD (X-ray diffraction) and scanning electron microscopy (SEM). FTIR analysis showed interaction between bacterial cellulose nanobiocomposites and calcium phosphate and XRD demonstrated amorphous calcium phosphate and calcium chloride on bacterial cellulose nanobiocomposites. SEM images confirmed incorporation of calcium phosphate in bacterial cellulose nanobiocomposites surface with different calcium phosphate particles morphology.

Surface engineering of silica nanoparticles for oral insulin delivery: characterization and cell toxicity studies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bone morphogenetic proteins: promising molecules for bone healing, bioengineering, and regenerative medicine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Synthesis and evaluation of novel prenylated chalcone derivatives as anti-leishmanial and anti-trypanosomal compounds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration

Bacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites.

Compatibilidade entre alginato e gesso ortodônticos: capacidade de umedecimento sob influência da desinfecção do molde

The dental trade has offered dental impression and dental stone for orthodontic use ensuring accurate models. The compatibility between these materials is defined by the wetting of the model surfaces by the mixture water/stone poured over it and the influenced by the method of disinfection of model and disinfectant solution used. It was evaluated the influence of spray disinfection with sodium hypochlorite 1% on the wettability of two commercial alginate (Jeltrate ® - Dentsply and Orthoprint ® - Zhermack) at two commercial type III gypsum (Rio ® - ME and AOBussoli Orthogesso Orthogesso ®-SA). Twenty models were fabricated for each type of alginate, which were divided into two groups (water and sodium hypochlorite), receiving respectively water and sodium hypochlorite 1% spray. Each group of models was then further divided into two subgroups, and on their surface were poured 2 ml of type III gypsum (Gesso Rio® or Orthogesso®). Reached the final setting of the gypsum specimens were sectioned vertically and medially, settled water with sandpaper No. 400 and mounted on suitable device for reading (in the right and left) of the contact angle Carl Zeiss microscope (precision, 001). The results were submitted to ANOVA and founded statistical significance for solutions used. It was concluded that sodium hypochlorite spray improved wettability of alginates studied.

Efeito da desinfecção e do tempo de armazenagem na molhabilidade de moldes de alginato

The cares at the manipulation of the alginate (condensation, disinfection of the impression and the time elapsed until the leak of the plaster) deserve special attention considering the great amount of distortions occurring every time the impression is not fill of plaster in a brief space of time and appropriate storage conditions. Besides its adaptation to the plaster suffers influence of the events happened after the impression is removed of the mouth. The purpose of this research was to evaluate the wetting capacity of three brands of alginate (Jeltrate™,Hydrogum™ e Orthoprint™) by the plaster type III (Rio™) under the influence of disinfection by sodium hypochlorite 1% sprays and the time of storage of 15 minutes,30 minutes, 1 hour, 6, 12 and 24 hours. There were made 60 impressions of each brand of alginate divided in two groups (water and sodium hypochlorite 1%) rearranged after the application of the sodium hypochlorite spray according to the storage time (15 or 30 minutes and 1,6,12, or 24 hours). On the surface of the impressions 2ml of plaster were flowed, proportioned and condensed in agreement with manufacturer‘s instructions. After the final setting expansion the casts were sectioned vertically and medially, regularized at the cut surface (emery paper 400) and setted for reading the contact angle at the microscope Carl Zeiss. The obtained results, submitted to statistical treatment (ANOVA) revealed significant differences when compared the employed solutions (water and sodium hypochlorite 1%) and the time of storage. The sodium hypochlorite 1% exhibited the smallest contact angles and the times of storage of 15 minutes and 6 hours the smallest and larger angles, respectively. It can be concluded that the alginate impressions exhibited larger adaptation to the plaster when disinfected by hypochlorite of sodium 1% and stocked by 15 minutes.

Combining xanthan and chitosan membranes to multipotent mesenchymal stromal cells as bioactive dressings for dermo-epidermal wounds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos do LRP6 e Frizzled6 na diferenciação endotelial de DPSC

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Incorporação de nanoemulsões de óleos essenciais de melaleuca, copaíba e limão em filmes de alginato de sódio para utilização como curativo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)