Recovery of mutans streptococci on MSB, SB-20 and SB-20M agar media

Objective: The recovery of mutans streptococci in saliva and dental biofilm samples depends, in part, on the culture medium used. In this study, we compared (i) the culture media Sucrose-Bacitracin agar (SB-20), Modified SB-20 (SB-20M) and Mitis Salivarius Bacitracin agar (MSB) in the count of colony forming units (cfu) of mutans streptococci and (ii) in the morphological and biochemical differentiation between Streptococcus mutans and Streptococcus sobrinus. Design: Samples of non-stimulated saliva from 20 children were plated on SB-20, SB-20M and MSB, and incubated in microaerophilia at 37 °C for 72 h. Identification of microorganisms was based on analysis of colony morphology under stereomicroscopy. The biochemical identification of colonies was done by biochemical tests using sugar fermentation, resistance to bacitracin and hydrogen peroxide production. Results: There was no significant difference (p > 0.05) in the number of cfu of mutans streptococci recovered on SB-20 and SB-20M agar. Comparing the media, SB-20 and SB-20M yielded a larger number of mutans streptococci colonies (p < 0.05) and were more effective than MSB in the identification of S. sobrinus (p < 0.05), but not of S. mutans (p > 0.05). Conclusion: There was no significant difference between SB-20 and SB-20M culture media in the count of mutans streptococci, demonstrating that the replacement of sucrose by coarse granular cane sugar did not alter the efficacy of the medium. Compared with MSB, SB-20 and SB-20M allowed counting a larger number of mutans streptococci colonies and a more effective morphological identification of S. sobrinus. © 2012 Elsevier Ltd.

Report of cerebellar hypoplasia in three calves

Hereditary or acquired cerebellar hypoplasia (CH) is commonly diagnosed in Holstein, Guernsey, Shorthorn and Jersey cattle. Bovine viral diarrhea (BVD) has been associated to acquired CH due to viral infection during the second trimester of pregnancy. Stricken calf usually shows ataxia, hypermetria, opisthotonus, intentional tremor and wide-based stance when in standing position. Three newborn calves were referred to the FCAV/Unesp Veterinary Teaching Hospital because of neurological distress. The clinical presentation, similar in all cases, indicated CH. Two weeks later, clinical signs did not improve and euthanasia was performed. Macroscopic examination revealed a gelatinous serosanguineous fluid over the brain surface and within the cervical spinal canal. Histologically the cerebellum had disorganization of the internal granular layer and moderate disappearance of Purkinje cells. The observed clinical signs and nervous tissue lesions were consistent with congenital cerebellar syndrome, possibly associated to viral infection during fetal development. Despite CH has been assumed to be related to BVD, blue tongue and Akabane viruses, only the BVD etiology has been already identified in Brazil.

Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production

The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C - Control (nontreated), A - Artin M gel, and V - Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. © 2013 by the Wound Healing Society.

Disseminação de Rickettsia rickettsii nas glândulas salivares e ovários de fêmeas de Rhipicephalus sanguineus (Latreille 1806) (Acari:Ixodidae) por meio da aplicação de técnicas morfológicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morfometria do cerebelo de ratos machos UChA e UChB submetidos a separação materna neonatal (consumidores voluntários de etano)

Pós-graduação em Biologia Geral e Aplicada - IBB

Estudo de propriedades anelásticas de MgB2

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito da hidrólise enzimática seguida da moagem em moinho de bolas sobre as características estruturais e físico-químicas do amido de madioquinha-salsa

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação do desempenho de um reator anaeróbico compartimentado no tratamento de soro de queijo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Liberação das enzimas LDH e PepX e evolução da maturação de queijo parmesão adicionado de culturas autolíticas de Lactobacilus helveticus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características físico-químicas e estruturais de amidos nativos e suas dextrinas Naegeli

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da hidrólise enzimática do amido de mandioquinha-salsa (Arracacia xanthorrhiza): efeito do tamanho dos grânulos

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Métodos analíticos, numéricos e experimentais para o cálculo de ondas de impacto em meios líquidos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Morfologia, ultraestrutura e morfometria do tegumento da paca (Cuniculus paca)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da rugosidade e ultra-estrutura superficial do esmalte dental humano submetido a diferentes métodos profiláticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos de tratamentos hidrotérmicos nas propriedades viscográficas e amidos resistentes em féculas de mandioca

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)