Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production


Autoria(s): Kim, Yeon J.; Carvalho, Fernanda C.; Souza, João A.C.; Gonçalves, Pedro C.G.; Nogueira, Andressa V.B.; Spolidório, Luis Carlos; Roque-Barreira, Maria C.; Cirelli, Joni Augusto
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/05/2013

Resumo

The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C - Control (nontreated), A - Artin M gel, and V - Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. © 2013 by the Wound Healing Society.

Formato

456-463

Identificador

http://dx.doi.org/10.1111/wrr.12041

Wound Repair and Regeneration, v. 21, n. 3, p. 456-463, 2013.

1067-1927

1524-475X

http://hdl.handle.net/11449/75268

10.1111/wrr.12041

WOS:000318444000014

2-s2.0-84877579994

Idioma(s)

eng

Relação

Wound Repair and Regeneration

Direitos

closedAccess

Palavras-Chave #collagen fiber #interleukin 1 #interleukin 6 #lectin #transforming growth factor beta #vasculotropin #animal cell #animal tissue #cell proliferation #cheek mucosa #collagen synthesis #controlled study #enzyme linked immunosorbent assay #epithelization #fibroblast culture #gel #histology #immunohistochemistry #in vivo study #male #mouth lesion #nonhuman #priority journal #protein expression #rat #secretion (process) #surgical wound #wound healing
Tipo

info:eu-repo/semantics/article