Detecção molecular do rearranjo Line-1 /c-Myc em tumores venéreos transmissíveis caninos espôntaneos

Transmissible venereal tumor (TVT) is a neoplasia that develops naturally in dogs. It can be easily transplanted, which demonstrates its ability to spread from animal to animal. Since the Linr-1/c-MYC rearrangement in TVT cells had not been studied at the Veterinary Hospital of the Veterinary School, Unesp in Botucatu, SP, this study aimed to detect this genetic alteration specific to this kind of tumor by means of the polymerase chain reaction (PCR). Twenty dogs with cytological diagnosis of TVT were used. Samples of neoplastic cells were collected to determine the presence of the Line-1/c-MYC marker. The rearrangement characterized by 340bp amplicons did not vary, in agreement with previous studies using the same methodology. This contributed to a more precise identification of persistent tumor cells in cases in which gross or microscopical detection was not possible.

Localizador de faltas por ondas viajantes em linhas de transmissão

It is important to ensure smoothly operation of a transmission line, sending energy to the consumption centers with minimal interruptions and safer. With this necessity, the fault location techniques have grown, in order to mitigate the impact of the fault and its correction can be more quickly and accurately. This paper presents a comparison between two different techniques of fault location, the fault location by impedance method, which uses the line parameters, the impedance per distance, voltage and current signals, and the other is the traveling wave method, which uses only a very fast analysis of time, synchronization and length between the line ends, do not using voltage and current values, but the temporal perception of the fault. Using travelling waves fault detection makes the previous knowledge of line parameters obsolete, which helps a lot network technicians and engineers with a quick and easy analysis. The methods are simulated in ATP Draw software, so both algorithms can be evaluated for their effectively and accuracy