154 resultados para Catalisadores : Complexos de paládio


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Introduction: Tuberculosis (TB) is a granulomatous disease caused by Mycobacterium tuberculosis. The genus Mycobacteriumhas two different complexes: M. tuberculosis Complex and M. avium Complex. This is a global health epidemic and remains a major global health problem, besides, the clinical severity of TB is significantly higher in transplanted patients. The detection of these mycobacteria complexes in transplanted patients, by molecular methods, is fundamental for quick treatment of patients and can contribute for rapid and accuracy of diagnosis. Objective: To detect mycobacteria DNA of M. tuberculosis and M. avium Complexes in formalin fixed paraffin-embedded samples (FFPE) of two patients groups: non transplanted and transplanted. Materials and Methods: The study includes 40 FFPE biopsies separated in four groups: NTP – presence of epithelioid granuloma and positive ZN, non-transplanted patients – 9 samples; NTN - presence of epithelioid granuloma and negative ZN, non-transplanted patients – 10 samples; TP – positive ZN, transplanted patients – 9 samples; TN – negative ZN, transplanted patients – 7 samples. Sections were cut for DNA extraction. Samples were submitted to PCR for amplification of: a) β-actin, b) IS6110 insertion and c) IS1245 insertion. DNA evaluation was made by spectrophotometry and efficiency and PCR analysis was made by agarose gels under UV light. Results: In all samples processed, 97.1% were positive for human β-actin gene. In22.2% of NTP group were found the IS6110 insertion sequencebut the IS1245 wasn´t. In the NTN group was not found any sequence. In theTP group, 11.1% of the samples were positive for IS6110 and also 11,1% werepositive for IS1245. In the TN group, 14.3% of the samples were positive forIS6110 and for IS1245, 14.3% was also positive. Conclusion: Although factors such as DNA degradation after formalin fixation and paraffin embedding, were possible to detect DNA from the human gene ...

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Nos últimos anos, a biotecnologia tem proporcionado avanços no desenvolvimento de biomateriais a serem utilizados como dispositivos médicos. Dentre esses dispositivos, podemos destacar aqueles que promovem a reparação tecidual e, também, a liberação controlada de fármacos. Partindo deste propósito, lentes de contato à base de celulose bacteriana (CB) foram desenvolvidas, visando o efeito curativo e reparativo para doenças da superfície ocular. A adição de ciclodextrinas (CDs) e fármacos, como o ciprofloxacino (CP) e o diclofenaco de sódio (DS) á essas lentes proporcionando propriedades terapêuticas e de liberação controlada dos fármacos. No entanto, para certificar-se do uso seguro desses compósitos pela indústria de dispositivos médicos, é necessário verificar se não possuem atividade citotóxica, genotóxica e/ou mutagênica. Sendo assim, a proposta do presente trabalho foi o desenvolvimento de uma adição eficaz de fármacos às lentes de contato de CB, para que sejam liberados de maneira controlada e, também, a verificação de sua biossegurança. Para essa verificação foram realizados ensaios in vitro de citotoxicidade (ensaio com o kit XTT e Sobrevivência Clonogênica), genotoxicidade (ensaio Cometa) e mutagenicidade (teste do Micronúcleo) com os respectivos. Após a análise estatística dos resultados foi possível verificar que o material CB-CP apresentou efeito citotóxico e os materiais CB-DS e CB-CD-DS apresentaram efeito citotóxico, genotóxico e mutagênico. Essa informação é fundamental para uma futura utilização segura desse biomaterial pela indústria de dispositivos médicos ...

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Solid-state compounds of general formula LnL3.nH2O, in wich Ln represents lanthanum, lanthanides and yttrium, L is ketoprofen, and n = 0,5 (Pr, Sm, Tb), 1 (La, Eu, Dy, Ho, Er, Tm, Lu) e 1,5 (Ce, Nd, Gd, Yb, Y) were synthesized. Simultaneous thermogravimetry-differential analysis (TG-DTA), differential scanning calorimetry (DSC), X-ray powder diffractometry, infrared spectroscopy and EDTA complexometry were employed to characterize these compounds. The TG-DTA and DSC curves provided information concerning the thermal behaviour and thermal decomposition of synthesized compounds. The experimental and theoretical infrared spectroscopic data suggested that ketoprofen acts as a bidentate ligand towards trivalent lanthanides and yttrium (III)

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This Final Paper had as it main goal to make a thermoanalytical study of lighter trivalent lanthanides (Lanthanum, Cerium, Praseodymium, Neodymium, Samarium and Europium) with the Ibuprofen ligand (nonsteroidal anti-inflammatory) that have a general formula LnL3.nH2O, on solid state, where Ln are the Lanthanides, L is the Ibuprofen ligand and n = number of water molecules of hydration that went from 1,0 to all the compounds. In order to characterize this compounds, it has been used the thermoanalytical techniques TG-DTA (thermogravimetry - Diferential Thermal Analysis) and DSC (Diferential Scanning Calorimetry), Fourier transformed infrared spectroscopy (FTIR) and complexometric titration with EDTA. Through the TG-DTA technique, it has been possible to set the thermal stability of the compounds, the number of thermal decomposition steps and temperatures that ocurred that also provided stoichiometry to the synthesized compounds. The DSC technique has shown the enthalpy of dehydration of the samarium and europium compounds, it was not possible to see it in the other compounds due to a endothermic peak on the DSC curve not being formed. In the case of neodymium, a thermal event ocurred, in which it could be a oxidative decarboxylation right after the dehydration. The infrared was utilised to study the carboxilate groups streches, and so, suggest a ligand metals compound coordination, that to this present paper has been a bidentade bridged coordenation. At last, the complexometric tritation was used to very the ammount of metal present in each compound, and so, verify if the proposed stoichiometry was according to the theory

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This research presents a study of structural complexes formed between the bothropstoxin BthTX-I and PrTX-I and phospholipase A2 (PLA2s) inhibitor 12- methoxy-4-methyl-voachalotine (MMV) of Tabernamontana catharinensis and rosmarinic acid (Cv-RA) of Rosmarinus officinalis. For both were used cocrystallization experiments with the toxins and inhibitors. In the future, the experimental three-dimensional structures of these complexes will be obtained through the technique of crystallography. Simultaneously, construction occurred in silico of inhibitors Cv-RA and MMV and attempts of docking between these binders and protein structures. These theoretical data provide important information regarding the nature of interactions between inhibitors, toxins and structural changes induced by the protein molecule inhibitors. Therefore, the acquisition of this information has great value to identification, characterization and development (drug design) of compounds with great potential for biotechnology and drug use. Considering further and more relevant aplications, these compounds could be used to control symptoms in many cases of ophidic poisoning and also in combating of pathological processes (degenerative inflammatory diseases and auto-immune diseases, Alzheimer's disease, schizophrenia, among others) whose causes are related to molecules belonging to the same groups of proteins which are classified bothropstoxin that were studied in this project

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This paper proposes to assess the lability of complexes of uranium in soil using the technique of Diffusion in Thin Films by concentration gradients (DGT) in the vicinity the Osamu Utsumi mine, located in the municipality of Caldas - MG. The technique is based on the 1ª Fick Law, where metal complexes diffuse through a gel, which in turn is adsorbed on a resin. The metals adsorbed are recovered by an analytical technique and quantified by optical emission spectrometer with plasma inductively coupled (ICP-OES) or mass spectrometry with inductively coupled plasma (ICP-MS)

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O câncer, manifestação originada pelo crescimento descontrolado de células, afeta milhões de indivíduos. Os macrófagos são as primeiras células a serem ativadas para participar de uma resposta imunológica propriamente dita, são células capazes de secretar mais de cem produtos biologicamente ativos, entre esses, espécies reativas de nitrogênio e citocinas que atuam no contexto da resposta imunológica e/ou inflamatória. Sabendo que compostos de paládio (II) podem apresentar potenciais atividades antitumorais, neste trabalho foram testado os compostos de fórmula geral PdI2 (tdmPz) e Pd (SCN)2 (tdmPz), quanto a atividade anti-inflamatória e antitumoral. Como droga padrão das reações realizadas foi utilizada a cisplatina. Foi determinada a ação destes compostos frente ao sistema imunológico através, de ensaios de determinação de índice de citotoxicidade mediano (IC50) pela técnica de MTT, óxido nítrico (NO) e determinação das citocinas pró-inflamatórias TNF- e IL-12. Além disso, foi determinada a atividade antitumoral dos compostos frente à célula tumoral de Ehrlich. Os resultados não mostraram produção de NO e das citocinas IL-12 e TNF- pelos macrófagos peritoneais de animais normais e animais portadores do tumor de Ehrlich na sua forma sólida. No que se referem à atividade tóxica dos compostos testados, estes mostraram efeito citotóxico sobre os macrófagos e sobre a linhagem tumoral testada. Sendo assim mesmo não havendo a produção de mediadores próinflamatórios, os compostos apresentaram uma considerável citotoxicidade frente às células tumorais de Ehrlich e macrófagos peritoneais

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The Mangabal Sul and Mangabal Norte mafic-ultramafic complexes are interpreted as intrusive stratiform bodies in the Goiás Magmatic Arc during the Brazilian cycle, being economically important for harboring significant amounts of nickel and copper sulfides. The main lithotypes of the complexes are gabbronorites, olivine gabbronorites, pyroxenites and peridotites, with variated degrees of deformation, recrystallization and metamorphism superimposed, with metamorphic peak of amphibolite to granulite facies evidenced mainly by the occurrence of coronitic olivine in metamafic rocks and the occurrence of syn-kinematic retrometamorphism associated with the development of the main foliation Sn. The Sn foliation planes show NE-SW preferential direction, consistent with the foliation direction of VIII the enclosing gneisses and schists, also concordant with the general elongation of mafic and ultramafic bodies displayed on map. The sulfide phase presents textures that indicate remobilization, associated with the occurrence of significant amounts of rutile within the ore which reinforces this idea. Along with the sulfides, the occurrence of expressive quantities of titanium oxides such as ilmenite and rutile, make the area more economically attractive. It can be suggested that the Mangabal Norte and Mangabal Sul complexes are contemporary, have the same genetic affinity and suffered the same deformational and metamorphic processes, evidenced by their structural and petrological similarities

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Blood is one of the most important and powerful elements in magical and religious symbology. As it is an essential substance for animal survival, it does not sound strange that through the ages blood has been given many significant symbolic values (both positive and negative). It can provide life, protection and prosperity in the same proportion that it can cause calamities, destruction and even death. In Opus agriculturae, a farming treatise written by Palladius (V AD), more specifically in Book I, Chapter 35, the author presents prescriptions to protect farms against scourges and climatic phenomena. He mentions some procedures in which blood is a fundamental component in the success of the prescriptions. The aim of the present article is to identify any magic power associated with these practices, their specific symbolic value, especially when related to menstrual blood and to other feminine elements with some magical or religious value. An evaluation is also made of the extent of these symbologies in the context of other practices and situations linked not only to agricultural magic, but to religion and to the ritual practices of other communities, whether in ancient times or not.

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This work analyses the chapter 35, book I, of the agricultural treatise Opus agriculturæ, written by Rutilius Taurus Aemilianus Palladius (V C.E.). In that chapter the author presents some recipes, called remedia, to protect the farm and the garden against weeds and weather phenomena, as blight and fogs. The magical practices are identified according to both the fundamental principles of magic (similarity, contiguity, contrariety), which rule magical thought, and some elements of magical symbology. As the author seems not to distinguish magic and science, for he brings together both kinds of recipes, the analysis of some remedia emphasizes a specific study on the materials and substances employed in those recipes and their value in Science today. This leads to the discussions of how magical thought works, what are the limits (if they actually exist) between Magic and Science and between Magic and Religion. This work covers the subjects above and it has the following pourposes: demonstrate the characteristics that authorize the remedia described by Palladius to be classified as folk magic; identify the relations between this kind of practice with more complex forms of magic, as with religion, with science; demonstrate the contribution of the folk magic in ancient Rome farming for the formulation of more apropriated criteria of evaluating magic in comparison to religious and scientific thought.

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Blood is one of the most important and powerful elements in magical and religious symbology. As it is an essential substance for animal survival, it does not sound strange that through the ages blood has been given many significant symbolic values (both positive and negative). It can provide life, protection and prosperity in the same proportion that it can cause calamities, destruction and even death. In Opus agriculturae, a farming treatise written by Palladius (V AD), more specifically in Book I, Chapter 35, the author presents prescriptions to protect farms against scourges and climatic phenomena. He mentions some procedures in which blood is a fundamental component in the success of the prescriptions. The aim of the present article is to identify any magic power associated with these practices, their specific symbolic value, especially when related to menstrual blood and to other feminine elements with some magical or religious value. An evaluation is also made of the extent of these symbologies in the context of other practices and situations linked not only to agricultural magic, but to religion and to the ritual practices of other communities, whether in ancient times or not.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)