153 resultados para Catch and Release


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Objetivou-se, com esse trabalho, avaliar a taxa de decomposição e a velocidade de liberação de macronutrientes e Si da fitomassa do consórcio crotalária mais milheto, em função do tempo após manejo, sem e com fragmentação. O experimento foi conduzido em condições de campo, em Botucatu-SP. O delineamento experimental foi de blocos casualizados, com quatro repetições, em esquema fatorial constituído por dois manejos da fitomassa da parte aérea (sem e com fragmentação mecânica) e seis épocas de coleta [0; 18; 32; 46; 74 e 91 dias após manejo (DAM)]. Foram determinadas a massa de matéria seca, teor de macronutrientes e Si e quantidade dos nutrientes remanescentes. Os dados foram submetidos à análise de variância, as médias dos tratamentos do fator manejo comparadas pelo teste de t (LSD) a 5% e do fator épocas de coletas ajustados a equações matemáticas. A fragmentação da fitomassa aumenta a taxa de decomposição e a liberação de N, P, Ca e S. O K é rapidamente disponibilizado, restando 91 DAM, em média, 1,5% da quantidade total acumulada. Aos 91 DAM pelo menos 80% de todos os macronutrientes já haviam sido liberados ao solo. A máxima liberação diária dos macronutrientes ocorre entre 0 a 18 DAM. A taxa de liberação de Si é constante e ocorre aumento do teor do elemento na fitomassa com o tempo devido à acentuada liberação de C. O Si é o elemento liberado mais lentamente ao solo, restando ainda 91 DAM, em média, 85% da quantidade total acumulada na fitomassa.

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This study describes the histology and histochemistry of the male reproductive system in Callinectes ornatus, comparing juvenile and adult developmental stages. We also analyzed changes in the gonadosomatic (GSI) and hepatosomatic (HSI) indices, and the weights of the testis and vas deferens during the development. The results showed that all stages, beginning with the juvenile (JUV), through developing (DEV) and mature (MAT) adult males of C. ornatus produce sperm and spermatophores. During development, testicular lobes showed the same characteristics of production and release of sperm into the seminiferous duct. The vas deferens showed little histological and histochemical change in the epithelium in juvenile and adult males. The differences consisted of the larger amount of secretion in MAT males compared to JUV and DEV ones. The chemical composition of the seminal fluid was similar, but MAT males produced a more homogeneous secretion. Morphological and physiological maturation are not synchronized in C. ornatus, since JUV males produced spermatophores similar to those in DEV and MAT males. However, these JUV are not yet able to reproduce, since they still have the abdomen attached to the cephalothoracic sternum. The increase of the GSI during development was significant for MAT males, and is related to the production of sufficient volume of seminal fluid to form the sperm plug in the female seminal receptacle. The HSI decreased from DEV to MAT adult stages, indicating that reserves from the hepatopancreas are used to develop the reproductive system after the pubertal molt.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Ciência e Tecnologia de Materiais - FC

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Apocynin, a methoxy-catechol originally extracted from the root of Picrorhiza kurroa, has been used as an inhibitor of the NADPH oxidase complex in phagocytic and nonphagocytic cells. Its mechanism of inhibition is linked to their prior activation through the action of peroxidases leading to oxidation of the dimeric product, diapocynin. In this study, dipocinina was synthesized and investigated its effect as an inhibitor of activation NADPH oxidase in neutrophils (PMN) and peripheral blood mononuclear cells (PBMC). The synthesis of diapocinina was performed by oxidation of apocinina by potassium persulphate in the midst of water for 5 minutes at room temperature. The precipitate was filtered and washed with water and methanol. Diapocinina was characterized by mass spectrometry. PMN and PBMC were obtained from peripheral blood of healthy donors and purified for gelatin sedimentation, or centrifugation with Histopaque ®, the red cells were lysed with ice water or ammonium chloride. Diapocinina or apocinina were incubated with opsonized zymosan, activation of PMNs and release of superoxide anion, these monitored by chemiluminescent assay dependent lucigenina. We found that diapocinina inhibitor was no better than the apocinina in PMN. However, diapocinina was more efficient than apocinina as an inhibitor of NADPH oxidase in PBMC. In conclusion, whereas PBMC are relatively poor compared with peroxidases PMN, our results are consistent with the need for oxidation apocinina for its effect as an inhibitor of NADPH oxidase

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