Principais marcadores utilizados na avaliação da qualidade do sêmen de animais domésticos

Methods of semen cryopreservation allow changes in spermatic cells, such as damage in plasma and acrossomal membrane and modifications in mitochondrial function due to a disorder in the lipidic bilayer. For effective oocyte fertilization, spermatozoa require functional competent membranes, and intact organelles, acrosome and DNA. However, most laboratory methods used to evaluate semen quality are not highly correlated with fertilizing capacity. The discovery of a variety of fluorochromes and compounds conjugated to fluorescent probes has enabled an accurate assessment of the viability, integrity and function of spermatozoa. Among the most used probes that label the various compartments of the sperm cell there are the membrane impermeable fluorescent dyes to test the membrane integrity, as well as acylated dyes that pass the intact membrane. For the acrossomal integrity the most commonly used method is lectins labeled by a fluorescent probe. The acrosome reaction and spermatic capacitation is detected by the evaluation of membrane architecture and disorder of lipids in plasma membrane. Mitochondrial function can be determined using markers for their aerobic activity. The DNA status of spermatozoa has been determined using the metachromatic properties of Acridine Orange, and the DNA fragmentation can also be assessed by TUNEL assay. Finally, DNA condensation is analyzed using a single cell DNA gel electrophoresis assay that indicates DNA compactation. This monograph aims to compile the various tests used to detect damaged spermatozoa under cryopreservation methods, searching for improve the predictive value of semen analysis with the intention of a successful conception

Fibrolytic enzyme production of Myceliophthora thermophila M.7.7. using inexpensive carbon sources and mineral nutrients

This study investigated the effect of inexpensive carbon and nitrogen sources on enzyme production by Myceliophthora thermophila M.7.7 in solid-state fermentation. Three kinds of lignocellulosic waste (corn straw, sugarcane bagasse and sugarcane straw) and six nitrogen sources (urea, calcium nitrate, analytical ammonium sulphate, yeast extract, agricultural fertilizer NPK 20-05-20 and fertilizing grade ammonium sulphate) were tested. Some physical-chermical parameters of the fermentation, such as temperature, initial pH and moisture content of the substrate on enzyme production, were evoluated. The maximum activities of xylanase (446.9 U/ml) endoglucanase (94.7 U/ml) and beta-glucosidase (2.8 U/ml) were observed in a mixture of corn straw and wheat bran (1:1 w/w) as the carbon source using fertilizer grade ammonium sulphate as the nitrogen source. This production occurred for an incubation period of 96 h, at 40°C, with initial moisture content of 70% and pH 5.0. These results have significant interest since they could be used for the future production of enzymes in a low-cost industrial process.

Crescimento e produção do maracujazeiro amarelo, sob diferentes fontes e doses de fósforo em cobertura

Brazil is the largest world producer of yellow passion fruit, a fruit crop which is exigent in nutrients, although little studied in relation to phosphorus fertilizing, fundamental on plant establishment. An experiment was carried out during the period of March/2010 to April/2011 in Remigio County, Paraiba State, Brazil, in order to evaluate the vegetative and productive attributes of yellow passion fruit plants under different sources and doses of phosphorus. Treatments were distributed in randomized blocks in tree replications and twelve plants by plot using the factorial design 2 x 5 referring the sources of superphosphate triple and superphosphate simple at levels P2O5 of 0, 12, 18, 24 and 30 g cave(-1). The following variables were recorded: stem diameter, number of productive branches, number of fruits per plant, fruit mass and fruit yield. The sources and doses of phosphorus affected growth and fruit production of yellow passion plants. The triple superphosphate is the most phosphorus efficient source for growth and fruit production of yellow passion fruit. The triple superphosphate dose which promoted the maximum yield of yellow passion fruit is 42.86 g.

Effect of antioxidants during bovine in vitro fertilization procedures on spermatozoa and embryo development

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fluxo de gases de efeito estufa na implantação de sistema silvipastoril em pastejo com ovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da adição de butil-hidroxitolueno nos meios de refrigeração e congelação sobre a viabilidade espermática de equinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de um sistema florestal de curta rotação de Eucalyptus spp. em função da desbrota e adubação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da centrifugação e filtragem do sêmen bovino sobre a criopreservação espermática

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)