A preliminary report on the effect of laser therapy on the healing of cutaneous surgical wounds as a consequence of an inversely proportional relationship between wavelength and intensity: Histological study in rats

Objective: The objective of the present investigation was to assess the histological effects of different wavelengths and intensities on the healing process of cutaneous wounds. Background Data: Tissue repair is a dynamic interactive process which involves mediators, cells and extra-cellular matrix. Several reports on the use of laser therapy have shown that the healing process is positively affected when the correct parameters are used. Methods: Eighteen standardized wounds were surgically created on the dorsum of male and female Wistar rats, which were subsequently divided into two experimental groups according to wavelength used λ.670 or λ685 nm) for lasertherapy (LLLT). Each group was divided into three subgroups of three animals according to the intensity of the applied irradiation (2,15, or 25 mW). Twelve animals were used as entreated controls and were not irradiated. The irradiation was carried out during seven consecutive days. The animals were sacrificed eight days after surgery. The specimens were removed, kept in 4% formaldehyde for 24 h, routinely prepared to wax, stained with H&E, and analyzed under light microscopy. Results: For both groups, light microscopy showed a substitution repair process; however, when LLLT was used, a positive biomodulatory effect was detectable, chiefly associated with shorter wavelength and low intensity. Conclusions: The results of the present study indicate that LLLT improved cutaneous wound repair and that the effect is a result of an inversely proportional relationship between wavelength and intensity, with treatment more effective when combining higher intensity with short wavelength or lower intensity with higher wavelength.

The effect of low intensity laser therapy on wound healing in Streptozotocin-induced diabetic rats

Diabetes Mellitus is a condition that results in a delay of the wound healing process, that is associated with an insufficient production of collagen, a decrease of the amount of collagen fibrils and deficient blood flow in the wound area. It is suggested that Low Intensity Laser Therapy acts by improving wound healing in normal organisms, accelerating tissue regeneration. The aim of this work was to investigate the biostimulatory effect of the HeNe laser irradiation, at 632.8 nm, on wound healing in 15 male rats suffering from diabetes induced by Streptozotocin, compared to 15 control diabetic animals. Irradiation parameters were: laser power of 15mW, exposition time of 17 s., irradiated area of 0.025 cm 2 and laser energy density of 10 J/cm 2. Full-thickness skin squared samples, with 5 mm of non-injured tissue around the wound, were obtained at 4, 7 and 15 days after wounding procedure (5 treated and 5 control animals each time). The histopathologic analysis performed by haematoxylin-eosin staining. Results suggested that the irradiation of diabetic rats was efficient for wound healing. Treated group presented better quality of the wound tissues by the macroscopic observation than control group and the microscopic analysis demonstrated that treated animals had better histopathologic evaluation than non treated.

Metal-insulator transition in Nd1-xEuxNiO 3 compounds

Polycrystalline Nd1-xEuxNiO3 (0≤x≤0.5) compounds were synthesized in order to investigate the character of the metal-insulator (MI) phase transition in this series. Samples were prepared through the sol-gel route and subjected to heat treatments at ∼1000 °C under oxygen pressures as high as 80bar. X-ray diffraction (XRD) and neutron powder diffraction (NPD), electrical resistivity ρ(T), and magnetization M(T) measurements were performed on these compounds. The NPD and XRD results indicated that the samples crystallize in an orthorhombic distorted perovskite structure, space group Pbnm. The analysis of the structural parameters revealed a sudden and small expansion of ∼0.2% of the unit cell volume when electronic localization occurs. This expansion was attributed to a small increase of ∼0.003 of the average Ni-O distance and a simultaneous decrease of ∼-0.5° of the Ni-O-Ni superexchange angle. The ρ(T) measurements revealed a MI transition occurring at temperatures ranging from TMI∼193 to 336K for samples with x ≤ 0 and 0.50, respectively. These measurements also show a large thermal hysteresis in NdNiO3 during heating and cooling processes, suggesting a first-order character of the phase transition at TMI. The width of this thermal hysteresis was found to decrease appreciably for the sample Nd 0.7Eu0.3NiO3. The results indicate that cation disorder associated with increasing substitution of Nd by Eu is responsible for changing the first-order character of the transition in NdNiO3. © 2006 IOP Publishing Ltd.

Depinning and vortex-glass-like transition in Nb with uncorrelated disorder

Studies of the third harmonic of the AC-susceptibility were conducted to detect the boundaries of the linear regime of the magnetic response of granular Nb samples. These studies reveal the extent of the region, on the phase diagram, where the magnetic response is linear, which corresponds to the disordered phase of Vortex Matter. The present work addresses the correlation between a linear response and experimental parameters such as the frequency and the amplitude of the excitation field. The order-disorder border has been extracted from the onset temperature of the third harmonic measured at low-frequencies and low-excitation fields in the presence of dc magnetic fields. © 2008 IOP Publishing Ltd.

Determination of broiler femur parameters at different growth phases

The objective of this experiment was to analyse macroscopically the femur and determine the biochemical values at 8, 22 and 42 days of age, producing basic results that can help to understand the pathogeny of the locomotor problems and the broiler physiologic growth. A total of 60 Cobb male broilers were distributed in three age groups (8, 22 and 42 days of age) of 20 birds. All macroscopic measurements, except the cranial compact layer increased (p<0.05) over the course of the ages. The cranial compact layer presented the biggest measure at 22 days of age. The ash percentage increased (p<0.05) until 22 days of age, but at 42 days of age this values decreased. Calcium and phosphorus percentage in ash increased (p<0.05) until 22 days of age and evidenced constancy from 22 to 42 days of age. The biomechanical adaptation capacity of femur was evidenced by the increase of the macroscopic measures over the course of the ages and the different behavior of cranial compact layer at 22 days of age suggested an adaptation attempt of the immature bone to muscle mass increase. The ash and mineral percentage confirmed that the relative growth occurs with more intensity until 21 days of age. The calcium and phosphorus percentage evidenced the physiologic balance acting on the deposition of these minerals in femur. © Asian Network for Scientific Information, 2011.

Kinetic parameters for thermal decomposition of supramolecular polymers derived from diclofenac-meglumine supramolecular adducts

Meglumine is an aminocarbohydrate able to form supramolecular adducts with organic acids. The recognition is based on hydrogen bonds and the structures resulting from the complexation have high solubility in water. This property has been exploited by the pharmaceutical industry in the improvement of existing drugs, and the successful example of this approach involves the poorly soluble non-steroidal anti-inflammatory drugs (NSAIDs). Investigation of the thermal behavior of adduct obtained from meglumine and the NSAID diclofenac revealed that a polymer-like material is formed from the self-assembly of diclofenac-meglumine adducts in the melt. This polymer showed a high molecular weight around 2.0×105kDa. The kinetic parameters for the thermal decomposition step of the polymer were determined by the Capela-Ribeiro non-linear isoconversional method. From data for the TG curves in nitrogen atmosphere and heating rates of 5, 10, 15 and 20°Cmin-1, the Eα and Bα terms could be determined, and consequently the pre-exponential factor, Aα, as well as the kinetic model, g(α). © 2012 Elsevier B.V.

Relationship between running intensity, muscle activation, and stride kinematics during an incremental protocol

Objective: To analyze the effect of running intensity on stride length (SL), stride frequency (SF), stride time (ST) and the electromyographic signal of the rectus femoris (RF), vastus lateralis (VL), vastus medialis (VM), tibialis anterior (TA), biceps femoris (BF) and gastrocnemius lateralis (GL) muscles. Methods: Nine well-trained runners performed an incremental protocol with an initial velocity of 10km.h-1, and increments of 1km.h-1 every 3minutes until exhaustion. The electromyographic activity, SL, SF, ST, inter-stride coefficient of variation, and association between kinematic and electromyographic parameters were calculated at 60%, 80% and 100% of maximum running velocity. Results: SL, SF and electromyographic activity of the RF, VM, VL and GL increased and the ST decreased with increased running speed. Electromyographic variability of VL and VM was higher than GL, and variability was lower in TA than all other muscles. The inter-stride variability of muscle activation was associated with kinematic parameters, and their variability, differently as running speed increased. Conclusion: The incremental protocol increased electromyographic activity differently among lower limb muscles; increased SF and SL, and decreased ST, without changing the variability of these variables. Muscle activation variability was correlated with kinematic parameters, but the relationships among these measures varied with running intensity. © 2013 .

Study of the glass transition temperature of as-s glasses for the fabrication of chalcogenide optical fibers

Values of glass transition temperature (Tg) and of linear expansion coefficient (α) for Asx S100-x glasses were measured in the range of concentrations 35 × 42. Because of the importance of the glass formation region 35 × 42 for the optical fibers elaboration, special attention was made on high-pure Asx S100-x glasses. For the glass in the range of 35 × 38, we measure Tg with the interval of x equal to 1 at.% of arsenic. We also measured the Tg values with the interval of x equal to 0.5 at.% of As. We obtained nonlinear behavior of Tg, reflecting the change in molecular composition of As-S glass in the glass composition range studied. The control of such parameters is important to produce optical fibers with specific numerical aperture. © 2013 The American Ceramic Society and Wiley Periodicals, Inc.

Calling behavior and parasite intensity in Treefrogs, Hypsiboas prasinus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Propriedades fotoluminescentes de complexos tetrakis (β-dicetonatos) de európio para aplicação como dispositivos conversores de luz

Pós-graduação em Química - IBILCE

Study of K beta X-ray emission spectroscopy applied to Mn((2-x))V((1+x))O4 (x=0 and 1/3) oxyspinel and comparison with XANES

Oxidation state and coordination of transition metal cations seems to be hard to assess when considering multiple cations, each one with different possible oxidation states. In fact, this is the case of the spineltype double oxides family. High resolution K beta X-ray fluorescence spectra were measured in Mn(2-x)V(1+4)O4 (x=0 and 1/3) spinels-type double oxides in order to determine the oxidation state and coordination of V and Mn cations. The relative intensity of radiative Auger effect KM2,3M4,5 to the total intensity and the integral absolute difference value were used as reference parameters for the characterization of Mn oxidation states. The coordination of Mn ions was inferred by the intensity of the K beta(5) line. In the case of V compounds, it was used as the intensity of the line K beta' relative to the total area of K beta region. The obtained results were further compared with X-ray absorption spectra analysis, showing good agreements regarding the oxidation state characterization. However, there were found some discrepancies in coordination, due to customary oversimplifications in the K beta(5) line origin. The obtained results might represent valuable and useful data for chemical scopes of characterizing spineltype oxides family. (C) 2013 Elsevier Ltd. All rights reserved.

Reliability of Cardiorespiratory Parameters During Cycling Exercise Performed at the Severe Domain in Active Individuals

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Scaling properties and universality in a ratchet system

Critical exponents that describe a transition from unlimited to limited diffusion for a ratchet system are obtained analytically and numerically. The system is described by a two dimensional nonlinear mapping with three relevant control parameters. Two of them control the non-linearity while the third one controls the intensity of the dissipation. Chaotic attractors appear in the phase space due to the dissipation and considering large non-linearity are characterised by the use of Lyapunov exponents. The critical exponents are used to overlap different curves of average momentum (dynamical variable) onto a single plot confirming a scale invariance. The formalism used is general and the procedure can be extended to different systems.

Effects of rumen-protected choline supplementation on metabolic and performance responses of transition dairy cows

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effect of prior exercise intensity on oxygen uptake kinetics during high-intensity running exercise in trained subjects

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)