Influência da smear layer e sua espessura na adesão e citotoxicidade de um cimento de ionômero de vidro modificado por resina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise histopatológica comparativa de polpas humanas após confecção de cavidades de classe I com turbina de alta velocidade ou laser de Er:YAG

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Citotoxicidade direta e transdentinária da clorexidina sobre células odontoblastóides MDPC-23 e análise de sua atividade antimicrobiana in vitro

Pós-graduação em Ciências Odontológicas - FOAR

Difusão transdentinária e citotoxicidade de sistemas adesivos autocondicionantes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do laser de baixa intensidade sobre células odontoblastóides in vitro e complexo dentino-pulpar in vivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Citotoxidade de cimentos resinosos sobre célular obtidas da polpa de dentes humanos (HDPCs) e odontoblastóides (MDPC-23)

Pós-graduação em Reabilitação Oral - FOAR

A Novel 785-nm Laser Diode-Based System for Standardization of Cell Culture Irradiation

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agents

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bleaching effectiveness, hydrogen peroxide diffusion, and cytotoxicity of a chemically activated bleaching gel

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Low toxic effects of a whitening strip to cultured pulp cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Concentrations of and application protocols for hydrogen peroxide bleaching gels: Effects on pulp cell viability and whitening efficacy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Novos parâmetros para o clareamento dental: avaliação da eficácia, citotoxicidade e efeitos moleculares

Pós-graduação em Reabilitação Oral - FOAR

Low toxic effects of a whitening strip to cultured pup cells

Fundacao de Amparo a Pesquisa do Estado de sao Paulo (FAPESP)

Responses of dental pulp cells to a less invasive bleaching technique applied to adhesive restored teeth

To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (a = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.

Relação entre materiais dentários e o complexo dentino-pulpar

Despite the strong valorization of the esthetics and its relationship with restorative materials, the biological principles of any clinical procedure are extremely important to maintain the vitality of the dentin-pulp complex. Dentin and pulp tissue are susceptible to different kinds of irritants such as toxins from microorganisms, traumatic procedures of cavity preparation, as well as toxic components released by restorative materials applied in non recommended clinical situations. Initially, the pulp responds to irritation by starting an inflammatory reaction which involves outward movement of dentinal fluid and intratubular deposition of immunoglobulins, upregulation of odontoblast activities, presence of immune cells and their cytokines as well as local expression of neuropeptides and chemokines. After these initial events, the inflammation process can be resolved associated or not to sclerotic dentin formation and reactionary dentin deposition. If high intensity offensive stimuli are applied to the dentin-pulp complex, death of odontoblasts takes place and consequently pulp ageing or even partial necrosis of this tissue may occurs. Thereby, clinicians need to be aware about the physiological and pathological features of the dentin-pulp complex as well as the possible biological consequences of different clinical procedures. In this way, the dentists should be able to carry out minimally aggressive operative techniques and to select the more appropriate restorative materials for each specific clinical situation in order to obtain excellent clinical results associated to the maintenance of pulp vitality.