Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene

An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.

Identifying problems for choosing suitable areas for installation of a new landfill through GIS technology: A case study

Selecting a suitable place to install a new landfill is a hard work. Bauru is a Brazilian municipality where the local landfill currently in use has a life span that is almost over, and the selection of a new area for a future landfill is crucial and urgent. Here we use a geographic information system (GIS) approach to indicate possible suitable areas for installing the landfill. The considered criteria were: river network and the respective buffer zone, relief, urban areas and their respective buffer zone, existence of Areas for Environmental Protection (AEPs), occurrence of wells and their respective buffer zones, existence of airports and their buffer zones, wind direction, and the road network and its respective buffer zone. Due the facts that (1) Bauru has an urban area relatively large in relation to whole municipal area, (2) Bauru has two airports, and (3) this area encompasses parts of three AEPs, the model showed that there are few areas suitable and moderately suitable in Bauru, and the greater part of the municipality is unsuitable to install a new landfill. Due to this important finding reported here, the local policymakers should consider the suitable or even moderately suitable areas for analysis in situ or look for other creative solutions for destination of the solid waste. We highly encourage the use of GIS in studies that seek suitable areas for future landfills, having found that SIG was a tool that allowed fast and precise work and generated an outcome sufficiently clear of interpretation.Implications: Solid waste (SW) management is one of the main environmental concerns nowadays. Landfilling SW is still the main practice to disposal of such material. However, for many regions, suitable places for landfilling are getting scarce. This study proved this situation for a populous place in a southeastern Brazilian region. This study also showed how the decision makers should manage the problem in order to minimize the amount of SW generated and delivered for the landfill. Massive investment in education is a critical issue to reach the proposed aim.

Rank Aggregation for Pattern Classifier Selection in Remote Sensing Images

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ferramentas para mediação de fontes de informação: avaliação sobre seus usos em bibliotecas universitárias nacionais e internacionais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Reliability of Web-Based Information in Inquiry Projects

This case study aims at identifying how a community of secondary school students selects web-based information and factors associated with the reliability of online reference sources during their collaborative inquiry (co-inquiry) projects. This study, conducted in a public secondary school in Brazil, focused on information literacy skills for collaborative open learning (colearning). The research is based on qualitative content analysis implemented on the online platform weSPOT. Although students are mindful of the importance of comparing different sources of information they seem not to be aware of reliability in online environments. Teacher's guidance is essential to support co-learners in developing competences, particularly related to critical thinking.

Extracting Information from Qubit-Environment Correlations

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Multimessenger search for sources of gravitational waves and high-energy neutrinos: Initial results for LIGO-Virgo and IceCube

We report the results of a multimessenger search for coincident signals from the LIGO and Virgo gravitational-wave observatories and the partially completed IceCube high-energy neutrino detector, including periods of joint operation between 2007-2010. These include parts of the 2005-2007 run and the 2009-2010 run for LIGO-Virgo, and IceCube's observation periods with 22, 59 and 79 strings. We find no significant coincident events, and use the search results to derive upper limits on the rate of joint sources for a range of source emission parameters. For the optimistic assumption of gravitational-wave emission energy of 10(-2) M(circle dot)c(2) at similar to 150 Hz with similar to 60 ms duration, and high-energy neutrino emission of 1051 erg comparable to the isotropic gamma-ray energy of gamma-ray bursts, we limit the source rate below 1.6 x 10(-2) Mpc(-3) yr(-1). We also examine how combining information from gravitational waves and neutrinos will aid discovery in the advanced gravitational-wave detector era.

Brewer's yeast and sugarcane yeast as protein sources for dogs

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo hodológico e neuroquímico das aferências da região noradrenérgica A5 aos neurônios da raiz coclear

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

O ensino de ciências e as tecnologias digitais: competências para a mediação pedagógica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise multicriterial aplicado na definição de áreas prioritárias ao uso agrícola da terra da bacia do Rio Capivara, Botucatu, SP

The use of geographic information systems (GIS), combined with advanced analysis technique, enables the standardization and data integration, which are usually from different sources, allowing you to conduct a joint evaluation of the same, providing more efficiency and reliability in the decision-making process to promote the adequacy of land use. This study aimed to analyze the priority areas of the basin agricultural use of the Capivara River, Botucatu, SP, through multicriterial analysis, aiming at conservation of water resources. The results showed that the Geographic Information System Idrisi Selva combined with advanced analysis technique and the weighted linear combination method proved to be an effective tool in the combination of different criteria, allowing the determination of the adequacy of agricultural land use less subjective way. Environmental criteria were shown to be suitable for the combination and multi-criteria analysis, allowing the preparation of the statement of suitability classes for agricultural use and can be useful for regional planning and decision-making by public bodies and environmental agents because the method takes into account the rational use of land and allowing the conservation of hydrics resources.