84 resultados para Penicillium chrysogenum. Planejamento experimental. CMCase. Avicelase. Xilanase. FPase
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This work was developed from a literature search in order to provide teachers of physics theoretical and methodological requirements for the use of experimentation in physics teaching, with the aim of contributing to the teaching practice of these teachers and, consequently, for improving teaching this discipline. Therefore, to deal in the course of this research, the goals and types of practical activities, new information and communication technologies that fit the experimental approaches and the role of the teacher in this context, specifying the variables that influence and relate with experimental activities. Studies are presented on the methodology and planning of experimental activities to draw attention to the teacher and the important issues relevant to the preparation of these activities as, for example, the proposed objectives and types of discourse and existing approaches for the conduct of practical activities. In this sense, the trend is highlighted for walks where the approach of practical activities, within the context of practices interactionist and are pointed out strengths and weaknesses of different types of activities presented. We also highlight aspects of the learning process associated with some theoretical reference that are on that subject, as Piaget, Vigotski and Ausubel, so that the teacher can apply the concepts in their theories worked in the practical lessons of physics
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A company must have full knowledge and control of its operations so as to meet the market requirements and meet their production goals. Thus this paper uses the Taguchi method to extend the operational control of a cutting process by fusion of a synthetic fabric in the longitudinal direction. For process analysis and tracking of possible causes of the problem techniques of Production Engineering as the cause and effect diagram, also known as Ishikawa diagram, and design of experiments were used, the last one was applied to the design techniques of Taguchi. Finally the preparation method of understanding and design of experiment was due to the use of the software MINITAB v15 , which showed that the speed of rolling the fabric after cutting is crucial for controlling the entire operation
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This dissertation has as main theme the discuss about how the use of mathematical models for process optimization. The current scenario of strong competition to conquer the consumer market necessitates the development of improvements to better performance of the process as a whole, is to reduce costs, increase efficiency or effectiveness. Thus, the use of methodologies to assist in this process is becoming increasingly viable. Methodologies developed in the past are being studied and improved. An example is the Desirability, the object of the present study, which was developed in the 80's and has been improved over time. To understand and study this methodology was applied to the desirability function in three instances, where it was used Design of Experiments (DOE), taken from scientific papers, using the Solver tool (Excel ) and desirability (Minitab ). Thus, in addition to studying the methodology, it was possible to compare the performance of tools used for optimization in different situations. From the results of this study, it was possible to validate the superiority of one of the models studied compared fairly
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Atualmente, atender as necessidades dos consumidores uma das metas mais importantes, os consumidores esto em busca de produtos com qualidade e preos mais acessveis, para isso, indispensvel que as empresas se atualizem para melhorar seus produtos e servios. Com este cenrio, as superligas esto cada vez mais ganhando mercado, pois possuem timas propriedades, principalmente em relao a operar em temperaturas elevadas, podendo proporcionar maior eficincia para motores que necessitam trabalhar em altas temperaturas. Em contra partida a essa vantagem, as superligas possuem uma baixa usinabilidade, sendo importante a anlise do processo de usinagem para se tornarem mais aplicveis. Este trabalho visa otimizao do processo de torneamento cilndrico da superliga Nimonic 80A, com o intuito de melhorar a qualidade do produto, utilizando o Mtodo de Taguchi, com o arranjo ortogonal L16, sendo o comprimento de corte definido como varivel resposta e analisados seis fatores que poderiam influenciar na sua variao, tais fatores so: velocidade de corte, avano, profundidade de corte, tipo de pastilha, lubrificao e dureza do material. Os resultados obtidos demonstraram que os fatores avano, tipo de pastilha e lubrificao so significativos e exercem influencia no processo, sendo que o avano deve ser ajustado no nvel de 0,12 mm/rev, a pastilha a ser utilizada deve ser CP250 e a lubrificao deve ser feita de maneira abundante, para a otimizao do processo. Com a anlise dos resultados, tambm podemos observar a eficincia e confiabilidade do mtodo utilizado, mostrando resultados coerentes
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES)
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Ps-graduao em Engenharia Mecnica - FEG
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Ps-graduao em Engenharia Mecnica - FEG
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Na busca de novos recursos genticos capazes de produzir enzimas celulolticas a baixas/mdias temperaturas, o continente Antrtico vem demonstrando ser um ambiente bastante promissor. Neste contexto, o objetivo do presente trabalho foi avaliar a influncia de diferentes fatores na produo de celulases por fungos filamentosos isolados de amostras da Antrtica visando otimizao do processo possvel aplicao das mesmas na produo de etanol de segunda gerao. Foram utilizados os fungos L1-1 e E5B da Central de Recursos Microbianos da UNESP (CRM-UNESP) os quais foram previamente selecionados devido ao potencial celuloltico. O delineamento experimental foi utilizado para analisar a influncia de variveis independentes na produo enzimtica. A quantificao da celulase foi realizada pelo mtodo do cido dinitrosaliclico (ADNS). Antes de iniciar aplicao dos planejamentos experimentais, foram adotadas estratgias para tentar minimizar e otimizar ao mximo o potencial dos isolados, as quais resultaram no estabelecimento da melhor agitao e a temperatura para a produo de celulase em 150 rpm e 20C, para os dois isolados estudados. Inicialmente os fungos E5B e L1-1 apresentavam suas produes enzimticas em 0,233U/mL e 0,342U/mL, respectivamente (antes da aplicao dos desenhos experimentais). Durante a conduo do planejamento experimental do tipo Plackett&Burman(PB), foi verificada a preferncia dos isolados pela fonte de carbono glicose, com efeito significativo na produo de celulases para os dois isolados. Tendo em vista o seu elevado custo comercial, foram realizados estudos com a sacarose, uma fonte de carbono alternativa e mais barata, bem como indutores enzimticos. Aps trs planejamentos experimentais do tipo PB, foi selecionado o isolado L1-1 como o melhor produtor da enzima celulase. Aps a conduo de um quarto planejamento experimental do tipo Fatorial Fracionado 24-1, as...
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The aim of the present study was to verify, through the response surface methodology, the optimum conditions for the combined use of aqueous extract of white lupine (Lupinus albus L.) and tropical pitanga juice (Eugenia unifl ora L.) in the development of a drink. Therefore, the independent variables were represented by the volume of aqueous extract of lupine (mL) and by the volume of pitanga juice (mL). The dependent variables (responses) were obtained through sensory test of acceptance (appearance, aroma, taste and overall acceptability). The combined optimization of the variables pointed the second formulation (50mL of aqueous extract of lupine and 30mL of pitanga juice) of the experimental project as the most adequate. In conclusion it was possible to get a drink with attractive sensory characteristics using aqueous extract of white lupine and tropical pitanga juice. The usage of response surface methodology made possible the determination of regions of maximum acceptance for each attribute evaluated in the formulation of a drink with aqueous extract of white lupine and pitanga juice, with a minimum of tests.
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This study aimed to evaluate the use of sweet potato as a substrate for the production of spirits. In order to promote an alternative technologically feasible, an experimental design was performed to minimize the operations of preparation, defining the most adequate conditions for the fermentation process. From sweet potato flour obtained by milling and dehydration process of the roots was carried out an enzymatic process of hydrolysis-saccharification of suspension of sweet potato flour with 18% dry matter. The hydrolyzate was used in the fermentation process which followed the 23 full factorial experimental design with central and axial points, and the independent variables were the concentration of reducing sugars, concentration of viable yeast and fermentation temperature. The dependent variables were viable cells, residual sugar, ethanol, glycerol and methanol. The dependent variables were quantified by liquid chromatography. The data analysis indicate that the best fermentation conditions among the tested conditions were: concentration of yeast 5 x 107-1 x 108 in number of viable cells, total reducing sugars from 12.5 to 13.5% and temperature between 33 -34C.