Forskolin effect on the cryosurvival of in vitro-produced bovine embryos in the presence or absence of fetal calf serum

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Differential Expression of IGF Family Members in Heat-Stressed Embryos Produced In Vitro from OPU-Derived Oocytes of Nelore (Bos indicus) and Holstein (Bos taurus) Cows

Contents The IGF system is related to embryo quality. We aim to determine the effect of the heat stress on the mRNA expression of IGF1 and IGF2, IGFR1 and IGFR2, IGFBP2 and IGFBP4, and PAPPA in in vitro production (IVP) blastocysts from Nelore and Holstein after ovum pick up (OPU) to better understand the differences between these breeds.Oocytes from four Nelore and seven Holstein were collected in six OPU sessions. Following in vitro maturation and fertilization using six Nelore or Holstein sires, embryos were divided into control (cultured at 39 degrees C) and heat stress (HS; exposed to 41 degrees C for 9h). Blastocysts were submitted to RNA extraction. The IGF1 expression was higher in blastocysts under HS in both breeds, and the expression of IGFBP2 and IGFBP4 was higher in Holstein blastocysts under HS. The high PAPPA expression and the low expression of IGFBP2 and IGFBP4 are associated with a more efficient degradation of IGFBPs, which results in greater IGF bioavailability in Nelore blastocysts and may contribute to the superior HS tolerance in Nelore, when compared to Holstein.

Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uso de sêmen liofilizado de gatos domésticos (Felis silvestris catus) para produção in vitro de embriões através da técnica de injeção intracitoplasmática de espermatozóide (ICSI)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência da adição de fator de crescimento fibroblástico 10 (FGF10), durante a maturação oocitária, na produção in vitro de embriões bovinos

The Brazilian livestock stands out for having the world largest commercial herd of cattle and leads meat exportation and production of bovine embryos. The in vitro production (IVP) of embryos is considered an effective option to overcome problems such as infertility in cows with high economic value and also for genetic improvement of cattle. The in vitro oocyte maturation is an essential step to the success of IVP, but is still considered poor when compared to in vivo maturation. Recent studies have suggeested an important role of Fibroblast Growth Factor 10 (FGF10) on the in vitro maturation of oocytes, which favored the expression of genes related to oocyte maturation and cumulus cell expansion. Aware that maturity stage influences the final production of blastocysts, we aimed study to verify if the addition of FGF10 into the maturation medium is able to affect positively the IVP of bovine embryos. Hence, FGF10 was added to maturation in five different concentrations: 0.5 ng/mL (group 0.5), 2.5 ng/mL (group 2.5), 5 ng/mL (group 5), 10 ng/mL (group 10) and 50 ng/mL (group 50). Additionally, two other maturation groups were used, group BSA (Bovine Serum Albumin, 4 mg/mL) and group FCS (Fetal Calf Serum, 10%). The rates of cleavage, morula and blastocyst were analyzed by Analysis of Variance (ANOVA), differences of P<0.05 were considered significant. Cleavage rates did not differ between the seven groups. On the other hand, morula rate on FCS group was higher than groups BSA, 0.5, 10 and 50 (P<0.05), but did not differ among groups treated with intermediate doses of FGF10 (2.5 and 5). FCS group presented higher blastocyst rate compared to all other groups that were well below the FCS group (P<0.0001). Therefore, the use of FGF10 during oocyte maturation did not affect positively embryo development on the IVP of bovine embryos

Anormalidades fetais e placentárias decorrentes da produção in vitro de embriões bovinos

In vitro production of bovine embryo (IVP) has become a remarkable assisted reproduction biotechnology in Brazil, once it is considered an important tool for the genetic improvement of the herd. However, many abnormalities are associated with IVP technologies, such as higher incidences of embryo loss, abortions, hydrallantois and dystocia, prolonged gestation, increased birth weight and high perinatal mortality. Collectively, these abnormalities are known as “Large Offspring Syndrome”, which has limited the large-scale use of IVP technologies in cattle industry

Criopreservação de embriões bovino produzidos in vitro: estado da arte

In the last years, the embryo in vitro production for every domestic species and mainly for bovine has attained a notorius status. This reproductive biotechnical procedure associate with ultrasound-guided ovum pick up (OPU) has been more and more incorporated and spread in our cattle herds, ranking up Brazil already at the top of the list in number of in vitro embryo produced. Some significant advantages provided, such as the possibility of using the premature or pregnant animals oocytes, without necessarily requiring the use of hormonal treatment, to make it possible to generate pregnancy at a shorter period of time, the rationalization in the use of semen and optimization in the use of sexed semen were determinant factors for OPU/IVP to reach this outstanding position. Nevertheless, right now the possibility of IVP embryo cryopreservation, just now is the biggest impediment for maximizing the use of this biotechnology, due to both lack of efficient methods and low laboratory produced embryo cryotolerance. Nowadays, the most used methods of IVP embryo cryopreservation are: slow freezing and vitrification. Traditionally, slow freezing is still the most used methods for in vivo and in vitro produced embryo cryopreservation. However, more recently vitrification - although still not commercially used in large scale - has been presenting satisfactory results in IVP embryo cryopreservation, according to searches

Avaliação da taxa de concepção de novilhas e vacas (Bos taurus x Bos indicus) com o uso de sêmen sexado na inseminação artificial ou embriões produzidos in vivo e in vitro

Pós-graduação em Medicina Veterinária - FCAV

Controle e estimulação de crescimento folicular em doadoras da raça holandesa para a produção in vitro de embriões

Pós-graduação em Medicina Veterinária - FCAV

Oxygen tension affects histone remodeling of in vitro-produced embryos in a bovine model

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Membrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reduction in cytoplasmic lipid content in bovine embryos cultured in vitro with linoleic acid in semi-defined medium is correlated with increases in cryotolerance

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Potencial do cultivo in vitro e do xenotransplante na sobrevida e desenvolvimento de folículos ovarianos pré-antrais em matrizes de ruminantes domésticos em risco de morte: revisão de literatura

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)